Purification and characterization of the hyper-glycosylated extracellular α-glucosidase from Schizosaccharomyces pombe

Enzyme and Microbial Technology

Volumn 37, Issue 5, 2005, Pages 472-480

  Okuyama, Masayuki ^a   Tanimoto, Yoshihiro ^a   Ito, Tatsuya ^a   Anzai, Akiko ^a   Mori, Haruhide ^a   Kimura, Atsuo ^a   Matsui, Hirokazu ^a   Chiba, Seiya ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Glucosidase; Glycoside hydrolase family 31; Hyper glycosylated enzyme; Schizosaccharomyces pombe

Indexed keywords

ENZYMES; MAGNETIC SUSCEPTIBILITY; MOLECULAR BIOLOGY; PROTEINS; PURIFICATION; SUBSTRATES; SUGAR (SUCROSE);

Α -GLUCOSIDASE; GLYCOSIDE HYDROLASE FAMILY 31; HYPER-GLYCOSYLATED ENZYMES; SCHIZOSACCHAROMYCES POMBE;

CELL CULTURE;

ALPHA GLUCOSIDASE; CARBOHYDRATE DERIVATIVE; FUNGAL ENZYME; MALTOSE; MATOTRIOSE; SUGAR; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOHYDRATE ANALYSIS; DEGLYCOSYLATION; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME DEGRADATION; ENZYME GLYCOSYLATION; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SUBSTRATE; FUNGAL CELL; FUNGAL CELL CULTURE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; RESIDUE ANALYSIS; SCHIZOSACCHAROMYCES POMBE; SUPERNATANT; THERMOSTABILITY;

SCHIZOSACCHAROMYCES POMBE;

EID: 23744447380     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2004.06.018     Document Type: Article

References (46)

1. S. Chiba α-Glucosidase. Handbook of Amylases and Related Enzymes 1988 The Amylase Research Society of Japan/Pergamon Press Oxford pp. 104-16
2. S. Chiba Molecular mechanism in alpha-glucosidase and glucoamylase Biosci Biotechnol Biochem 61 1997 1233 1239
3. T.P. Frandsen, and B. Svensson Plant α-glucosidases of the glycoside hydrolase family 31. Molecular properties, substrate specificity, reaction mechanism, and comparison with family members of different origin Plant Mol Biol 37 1998 1 13
4. B. Henrissat A classification of glycosyl hydrolases based on amino acid sequence similarities Biochem J 280 1991 309 316
5. B. Henrissat, and A. Bairoch New families in the classification of glycosyl hydrolases based on amino acid sequence similarities Biochem J 293 1993 781 788
6. B. Henrissat, and G. Davies Structural and sequence-based classification of glycoside hydrolases Curr Opin Struct Biol 7 1997 637 644
7. M. Okuyama, A. Okuno, N. Shimizu, H. Mori, A. Kimura, and S. Chiba Carboxyl group of residue Asp647 as possible proton donor in catalytic reaction of α-glucosidase from Schizosaccharomyces pombe Eur J Biochem 268 2001 2270 2280
8. A. Kita, H. Matsui, A. Somoto, A. Kimura, M. Takata, and S. Chiba Substrate specificity and subsite affinities of crystalline α-glucosidase from Aspergillus niger Agric Biol Chem 55 1991 2327 2335
9. S. Chiba, S. Inomata, H. Matsui, and T. Shimomura Purification and properties of an α-glucosidase (glucoamylase) in sugar beet seed Agric Biol Chem 42 1978 241 245
10. T.P. Frandsen, F. Lok, E. Mirgorodskaya, P. Roepstorff, and B. Svensson Purification, enzymatic characterization, and nucleotide sequence of a high-isoelectric-point α-glucosidase from barley malt Plant Physiol 123 2000 275 286
11. H. Im, and C.A. Henson Characterization of high pI α-glucosidase from germinated barley seeds: substrate specificity, subsite affinities and active-site residues Carbohydr Res 277 1995 145 159
12. M. Rolfsmeier, and P. Blum Purification and characterization of a maltase from the extremely thermophilic crenarchaeote Sulfolobus solfataricus J Bacteriol 177 1995 482 485
13. Y. Banno, and Y. Nozawa Purification and characterization of lysosomal α-glucosidase secreted by eukaryote Tetrahymena J Biochem (Tokyo) 97 1985 409 418
14. R. Scheibe, K.W. Wenzel, and E. Hofmann Purification and some properties of lysosomal α-glucosidase of rat liver Biomed Biochim Acta 44 1985 1279 1286
15. F. Usuki, S. Ishiura, and H. Sugita Isolation and characterization of three α-glucosidases from the Japanese quail J Biochem (Tokyo) 99 1986 985 988
16. P.L. Jeffrey, D.H. Brown, and B.I. Brown Studies of lysosomal α-glucosidase. I. Purification and properties of the rat liver enzyme Biochemistry 9 1970 1403 1415
17. N. Kato, S. Suyama, M. Shirokane, M. Kato, T. Kobayashi, and N. Tsukagoshi Novel α-glucosidase from Aspergillus nidulans with strong transglycosylation activity Appl Environ Microbiol 68 2002 1250 1256
18. H. Heymann, and S. Gunther Calculation of subsite affinities of human small intestinal glucoamylase-maltase Biol Chem Hoppe Seyler 375 1994 451 455
19. K.A. Conklin, K.M. Yamashiro, and G.M. Gray Human intestinal sucrase-isomaltase. Identification of free sucrase and isomaltase and cleavage of the hybrid into active distinct subunits J Biol Chem 250 1975 5735 5741
20. J. Kolinska, and J. Kraml Separation and characterization of sucrose-isomaltase and of glucoamylase of rat intestine Biochim Biophys Acta 284 1972 235 247
21. r 540,000 homohexameric α-glucosidase with both exo-α-l,4-glucosidase and oligo-l, 6-glucosidase activities Eur J Biochem 245 1997 129 136
22. P. Le Chevalier, and A. Van Wormhoudt α-Glucosidase from the hepatopancreas of the shrimp. Penaeus vannamei (Crustacea-Decapoda) J Exp Zool 280 1998 384 394
23. H. Heymann, D. Breitmeier, and S. Gunther Human small intestinal sucrase-isomaltase: different binding patterns for malto- and isomaltooligosaccharides Biol Chem Hoppe Seyler 376 1995 249 253
24. M. Sugimoto, S. Furui, and Y. Suzuki Multiple molecular forms of α-glucosidase from spinach seeds, Spnacia oleracea L Biosci Biotech Biochem 59 1995 673 677
25. M. Sugimoto, and Y. Suzuki Subsite affinities of α-glucosidase from Mucor javanicus Biosci Biotech Biochem 57 1993 1378 1379
26. H. Matsui, S. Chiba, and T. Shimomura Substrate specificity of an α-glucosidase in sugar beet seed Agric Biol Chem 42 1978 1855 1860
27. C. Fanutti, M.J. Gidley, and J.S. Grant-Reid A xyloglucan- oligosaccharide-specific α-d-xylosidase or exo-oligoxyloglucan-α- xylohydrolase from germinated nasturtium (Tropeaolum majus L.) seeds Planta 184 1991 137 147
28. J.M. Mitchison Physiological and Cytological Methods for Schizosaccharomyces pombe. Methods in Cell Physiology vol. 7 1970 Academic Press pp. 131-65
29. M. Dubois, R. Gilles, J.K. Hamilton, P.A. Robers, and F. Smith Colorimetric method for determination of sugars and related substances Anal Chem 28 1956 350 356
30. S. Chiba, A. Kimura, and H. Matsui Quantitative study of anomeric forms of glucose produced by α-glucosidase and glucoamylase Agric Biol Chem 47 1983 1741 1746
31. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
32. A. Gattiker, W.V. Bienvenut, A. Bairoch, and E. Gasteiger FindPept, a tool to identify unmatched masses in peptide mass fingerprinting protein identification Proteomics 2 2002 1435 1444
33. M.R. Wilkins, E. Gasteiger, A.A. Gooley, B.R. Herbert, M.P. Molloy, and P.A. Binz High-throughput mass spectrometric discovery of protein post-translational modifications J Mol Biol 289 1999 645 657
34. C.A. Cooper, E. Gasteiger, and N. Packer GlycoMod - a software tool for determining glycosylation compositions from mass spectrometric data Proteomics 1 2001 340 349
35. K. Hiromi, Y. Nitta, C. Numata, and S. Ono Subsite affinities of glucoamylase: examination of the validity of the subsite theory Biochim Biophys Acta 302 1973 362 375
36. K. Hiromi Interpretation of dependency of rate parameters on the degree of polymerization of substrate in enzyme-catalyzed reactions. Evaluation of subsite affinities of exo-enzyme Biochem Biophys Res Commun 40 1970 1 6
37. O. Olsen, and K.K. Thomsen Improvement of bacterial β-glucanase thermostability by glycosylation J Gen Microbiol 137 1991 579 585
38. S. Barbaric, V. Mrsa, B. Ries, and P. Mildner Role of the carbohydrate part of yeast acid phosphatase Arch Biochem Biophys 234 1984 567 575
39. C. Wang, M. Eufemi, C. Turano, and A. Giartosio Influence of the carbohydrate moiety on the stability of glycoproteins Biochemistry 35 1996 7299 7307
40. P.M. Rudd, H.C. Joao, E. Coghill, P. Fiten, M.R. Saunders, and G. Opdenakker Glycoforms modify the dynamic stability and functional activity of an enzyme Biochemistry 33 1994 17 22
41. B.A. Bernard, S.A. Newton, and K. Olden Effect of size and location of the oligosaccharide chain on protease degradation of bovine pancreatic ribonuclease J Biol Chem 258 1983 12198 12202
42. S. Moreno, T. Ruiz, Y. Sanchez, J.R. Villanueva, and L. Rodriguez Subcellular localization and glycoprotein nature of the invertase from the fission yeast Schizosaccharomyces pombe Arch Microbiol 142 1985 370 374
43. B.Y. Reichelt, and G.H. Fleet Isolation, properties, function, and regulation of endo-(l → 3)-β-glucanases in Schizosaccharomyces pombe J Bacteriol 147 1981 1085 1094
44. T. Saeki, M. Okuyama, H. Mori, A. Kimura, and S. Chiba Localization of α-glucosidase in yeast cells Oyo Toshitsu Kagaku (J Appl Glycosci) 45 1988 281 283
45. S. Kashiwabara, S. Azuma, M. Tsuduki, and Y. Suzuki The primary structure of the subunit in Bacillus thermoamyloliquefaciens KP1071 molecular weight 540,000 homohexameric α-glucosidase II belonging to the glycosyl hydrolase family 31 Biosci Biotechnol Biochem 64 2000 1379 1393
46. M. Sugimoto, T. Ohta, and F. Kawai Change in maltose- and soluble starch-hydrolyzing activities of chimeric α-glucosidases of Mucor javanicus and Aspergillus oryzae Biochim Biophys Acta 1645 2003 1 5