Mutagenesis of Ala290, which modulates substrate subsite affinity at the catalytic interface of dimeric ThMA

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1751, Issue 2, 2005, Pages 170-177

  Park, Sung Hoon ^a   Cha, Hyunju ^a   Kang, Hee Kwon ^a   Shim, Jae Hoon ^a   Woo, Eui Jeon ^b   Kim, Jung Wan ^c   Park, Kwan Hwa ^a  

^a Seoul National University   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^c SolarLight Ltd   (South Korea)

Author keywords

Kinetic parameter; Maltogenic amylase (ThMA); Maltose; Thermus sp.

Indexed keywords

ALANINE; BACTERIAL ENZYME; DIMER; GLUCOSE; HYDROLASE; ISOLEUCINE; MALTOGENIC AMYLASE; MALTOSE; OLIGOSACCHARIDE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CATALYSIS; CONTROLLED STUDY; DIMERIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN HYDROLYSIS; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; THERMUS; THIN LAYER CHROMATOGRAPHY; WILD TYPE;

ALANINE; AMINO ACID SEQUENCE; BINDING SITES; CATALYTIC DOMAIN; GLUCOSE; GLYCOSIDE HYDROLASES; HYDROLYSIS; ISOLEUCINE; KINETICS; MALTOSE; METHYLGLUCOSIDES; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGOSACCHARIDES; PROTEIN BINDING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; THERMUS;

THERMUS; THERMUS SP.;

EID: 23444445889     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2005.05.004     Document Type: Article

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