A flow cytometry-based assay for the measurement of protein regulation of E-cadherin-mediated adhesion

Journal of Immunological Methods

Volumn 302, Issue 1-2, 2005, Pages 116-124

  Leong, Cheng Mee ^a   Hibma, Merilyn H ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Adhesion; E cadherin; Flow cytometry

Indexed keywords

CELL SURFACE PROTEIN; GREEN FLUORESCENT PROTEIN; UVOMORULIN;

ACCURACY; ARTICLE; CELL ADHESION; CELL SURFACE; CONTROLLED STUDY; FLOW CYTOMETRY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INCUBATION TIME; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; REGULATORY MECHANISM; REPRODUCIBILITY;

CADHERINS; CELL ADHESION; CELL AGGREGATION; CELL LINE, TRANSFORMED; FLOW CYTOMETRY; GENES, REPORTER; HUMANS; KERATINOCYTES; ONCOGENE PROTEINS, VIRAL; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; TRANSFECTION;

HUMAN PAPILLOMAVIRUS TYPE 16;

EID: 23444433255     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2005.05.001     Document Type: Article

References (26)

1. H. Aberle, H. Schwartz, and R. Kemler Cadherin-catenin complex: protein interactions and their implications for cadherin function J. Cell. Biochem. 61 1996 514
2. I.R. Beavon The E-cadherin-catenin complex in tumour metastasis: structure, function and regulation Eur. J. Cancer 36 2000 1607
3. W. Birchmeier, J. Behrens, K.M. Weidner, J. Hulsken, and C. Birchmeier Epithelial differentiation and the control of metastasis in carcinomas Curr. Top. Microbiol. Immunol. 213 Pt 2 1996 117
4. P. Boukamp, R.T. Petrussevska, D. Breitkreutz, J. Hornung, A. Markham, and N.E. Fusenig Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line J. Cell Biol. 106 1988 761
5. S. Chappuis-Flament, E. Wong, L.D. Hicks, C.M. Kay, and B.M. Gumbiner Multiple cadherin extracellular repeats mediate homophilic binding and adhesion J. Cell Biol. 154 2001 231
6. C.H. Damsky, J. Richa, D. Solter, K. Knudsen, and C.A. Buck Identification and purification of a cell surface glycoprotein mediating intercellular adhesion in embryonic and adult tissue Cell 34 1983 455
7. S.M. Frisch, and H. Francis Disruption of epithelial cell-matrix interactions induces apoptosis J. Cell Biol. 124 1994 619
8. L. Hinck, I.S. Nathke, J. Papkoff, and W.J. Nelson Dynamics of cadherin/catenin complex formation: novel protein interactions and pathways of complex assembly J. Cell Biol. 125 1994 1327
9. N. Keen, R. Elston, and L. Crawford Interaction of the E6 protein of human papillomavirus with cellular proteins Oncogene 9 1994 1493
10. K. Matthews, C.M. Leong, L. Baxter, E. Inglis, K. Yun, B.T. Backstrom, J. Doorbar, and M. Hibma Depletion of Langerhans cells in human papillomavirus type 16-infected skin is associated with E6-mediated down regulation of E-cadherin J. Virol. 77 2003 8378
11. A. Nagafuchi, and M. Takeichi Cell binding function of E-cadherin is regulated by the cytoplasmic domain Embo J. 7 1988 3679
12. B. Nagar, M. Overduin, M. Ikura, and J.M. Rini Structural basis of calcium-induced E-cadherin rigidification and dimerization Nature 380 1996 360
13. P. Navarro, M. Gomez, A. Pizarro, C. Gamallo, M. Quintanilla, and A. Cano A role for the E-cadherin cell-cell adhesion molecule during tumor progression of mouse epidermal carcinogenesis J. Cell Biol. 115 1991 517
14. M. Ozawa, H. Baribault, and R. Kemler The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species Embo J. 8 1989 1711
15. S. Pokutta, K. Herrenknecht, R. Kemler, and J. Engel Conformational changes of the recombinant extracellular domain of E-cadherin upon calcium binding Eur. J. Biochem. 223 1994 1019
16. L. Shapiro, A.M. Fannon, P.D. Kwong, A. Thompson, M.S. Lehmann, G. Grubel, J.F. Legrand, J. Als-Nielsen, D.R. Colman, and W.A. Hendrickson Structural basis of cell-cell adhesion by cadherins Nature 374 1995 327
17. H. Takeda, Y. Shimoyama, A. Nagafuchi, and S. Hirohashi E-cadherin functions as a cis-dimer at the cell-cell adhesive interface in vivo Nat. Struct. Biol. 6 1999 310
18. M. Takeichi Functional correlation between cell adhesive properties and some cell surface proteins J. Cell Biol. 75 1977 464
19. M. Takeichi Cadherins: a molecular family important in selective cell-cell adhesion Annu. Rev. Biochem. 59 1990 237
20. M. Takeichi Cadherins in cancer: implications for invasion and metastasis Curr. Opin. Cell. Biol. 5 1993 806
21. M. Takeichi Morphogenetic roles of classic cadherins Curr. Opin. Cell Biol. 7 1995 619
22. M. Takeichi, and S. Nakagawa Cadherin-dependent cell-cell adhesion J. Bonicacino M. Dasso J. Lippincott-Schwartz J. Harford K. Yamada Current Protocols in Cell Biology 1998 John Wiley & Sons New York 9.3.1 9.3.15
23. T. Tegoshi, M. Nishida, K. Ishiwata, T. Kobayashi, F. Uchiyama, K. Nabeshima, Y. Nawa, and N. Arizono E-cadherin and cadherin-associated cytoplasmic proteins are expressed in murine mast cells Lab. Invest. 80 2000 1571
24. M.J. Wheelock, C.A. Buck, K.B. Bechtol, and C.H. Damsky Soluble 80-kd fragment of cell-CAM 120/80 disrupts cell-cell adhesion J. Cell. Biochem. 34 1987 187
25. A.S. Wong, and B.M. Gumbiner Adhesion-independent mechanism for suppression of tumor cell invasion by E-cadherin J. Cell Biol. 161 2003 1191
26. T. Yoshioka, T. Masuko, H. Kotanagi, O. Aizawa, Y. Saito, H. Nakazato, K. Koyama, and Y. Hashimoto Homotypic adhesion through carcinoembryonic antigen plays a role in hepatic metastasis development Jpn. J. Cancer Res. 89 1998 177