Translational up-regulation of antifolate drug targets in the human malaria parasite Plasmodium falciparum upon challenge with inhibitors

Molecular and Biochemical Parasitology

Volumn 136, Issue 1, 2004, Pages 63-70

  Nirmalan, Niroshini ^a   Sims, Paul F G ^a   Hyde, John E ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

5 fluoro 2 deoxyuridine 5 monophosphate; 5 fluoroorotate; 5 fluorouracil; bovine serum albumin; BSA; CG; cycloguanil; DHF; DHFR (dhfr); dihydrofolate; dihydrofolate reductase (gene); dimethylsulfoxide; DMSO; FdUMP; FO; FU; MTHF

Indexed keywords

4,6 DIAMINO 1,2 DIHYDRO 2,2 DIMETHYL 1 [3 (2,4,5 TRICHLOROPHENOXY)PROPOXY] 1,3,5 TRIAZINE; ANTIMALARIAL AGENT; CYCLOHEXIMIDE; DIHYDROFOLATE REDUCTASE; DIHYDROFOLATE REDUCTASE INHIBITOR; FLOXURIDINE PHOSPHATE; FLUOROOROTIC ACID; FLUOROURACIL; FOLIC ACID; FOLIC ACID ANTAGONIST; MESSENGER RNA; PYRIMETHAMINE; TETRACYCLINE; THYMIDYLATE SYNTHASE; THYMIDYLATE SYNTHASE INHIBITOR;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; DRUG EFFECT; ENZYME ACTIVITY; ENZYME INHIBITION; GENE EXPRESSION; HUMAN; IC 50; MALARIA FALCIPARUM; NONHUMAN; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; TRANSLATION REGULATION; UPREGULATION;

ANIMALS; ENZYME INHIBITORS; FLUOROURACIL; FOLIC ACID ANTAGONISTS; PLASMODIUM FALCIPARUM; PROTEIN BIOSYNTHESIS; PYRIMETHAMINE; TETRAHYDROFOLATE DEHYDROGENASE; THYMIDYLATE SYNTHASE; TRIAZINES; UP-REGULATION;

BOVINAE; MAMMALIA; PLASMODIUM FALCIPARUM;

EID: 2342650008     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2004.02.013     Document Type: Article

References (32)

1. Sibley C.H., Hyde J.E., Sims P.F.G., et al. Pyrimethamine-sulfadoxine resistance in Plasmodium falciparum: what next? Trends Parasitol. 17:2001;582-588
2. Rathod P.K. Antimalarial agents directed at thymidylate synthase. J. Pharm. Pharmacol. 49:1997;65-69
3. Hyde J.E. The dihydrofolate reductase-thymidylate synthetase gene in the drug resistance of malaria parasites. Pharmacol. Ther. 48:1990;45-59
4. Nirmalan N., Wang P., Sims P.F.G., Hyde J.E. Transcriptional analysis of genes encoding enzymes of the folate pathway in the human malaria parasite Plasmodium falciparum. Mol. Microbiol. 46:2002;179-190
5. Chu E., Takimoto C.H., Voeller D., Grem J.L., Allegra C.J. Specific binding of human dihydrofolate reductase protein to dihydrofolate reductase messenger-RNA in vitro. Biochemistry. 32:1993;4756-4760
6. Chu E., Voeller D., Koeller D.M., et al. Identification of an RNA binding site for human thymidylate synthase. Proc. Natl. Acad. Sci. U.S.A. 90:1993;517-521
7. Ercikan-Abali E.A., Banerjee D., Waltham M.C., Skacel N., Scotto K.W., Bertino J.R. Dihydrofolate reductase protein inhibits its own translation by binding to dihydrofolate reductase mRNA sequences within the coding region. Biochemistry. 36:1997;12317-12322
8. Kitchens M.E., Forsthoefel A.M., Rafique Z., Spencer H.T., Berger F.G. Ligand-mediated induction of thymidylate synthase occurs by enzyme stabilization: implications for autoregulation of translation. J. Biol. Chem. 274:1999;12544-12547
9. Tanabe K., Mackay M., Goman M., Scaife J.G. Allelic dimorphism in a surface antigen gene of the malaria parasite Plasmodium falciparum. J. Mol. Biol. 195:1987;273-287
10. Brooks D.R., Wang P., Read M., Watkins W.M., Sims P.F.G., Hyde J.E. Sequence variation of the hydroxymethyldihydropterin pyrophosphokinase- dihydropteroate synthase gene in lines of the human malaria parasite, Plasmodium falciparum, with differing resistance to sulfadoxine. Eur. J. Biochem. 224:1994;397-405
11. Wang P., Read M., Sims P.F.G., Hyde J.E. Sulfadoxine resistance in the human malaria parasite Plasmodium falciparum is determined by mutations in dihydropteroate synthetase and an additional factor associated with folate utilization. Mol. Microbiol. 23:1997;979-986
12. Read M, Hyde JE. Simple in vitro cultivation of the malaria parasite Plasmodium falciparum (erythrocytic stages) suitable for large-scale preparations. In: Hyde JE, editor. Protocols in molecular parasitology. Totowa, New Jersey: Humana Press; 1993. p. 43-55.
13. Wang P., Sims P.F.G., Hyde J.E. A modified in vitro sulfadoxine susceptibility assay for Plasmodium falciparum suitable for investigating Fansidar resistance. Parasitology. 115:1997;223-230
14. Houghton J.A., Weiss K.D., Williams L.G., Torrance P.M., Houghton P.J. Relationship between 5-fluoro-2′-deoxyuridylate, 2′-deoxyuridylate, and thymidylate synthase activity subsequent to 5-fluorouracil administration, in xenografts of human colon adenocarcinomas. Biochem. Pharmacol. 35:1986;1351-1358
15. Rathod P.K., Leffers N.P., Young R.D. Molecular targets of 5-fluoroorotate in the human malaria parasite, Plasmodium falciparum. Antimicrob. Agents Chemother. 36:1992;704-711
16. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254
17. Childs G.E., Lambros C. Analogs of N-benzyloxydihydrotriazines: in vitro antimalarial activity against Plasmodium falciparum. Ann. Trop. Med. Parasitol. 80:1986;177-181
18. Spears C.P., Shahinian A.H., Moran R.G., Heidelberger C., Corbett T.H. In vivo kinetics of thymidylate synthetase inhibition in 5-fluorouracil-sensitive and 5-fluorouracil-resistant murine colon adenocarcinomas. Cancer Res. 42:1982;450-456
19. McFadden G.I., Roos D.S. Apicomplexan plastids as drug targets. Trends Microbiol. 7:1999;328-333
20. Budimulja A., Syafruddin, Tapchaisri P., Wilairat P., Marzuki S. The sensitivity of Plasmodium protein synthesis to prokaryotic ribosomal inhibitors. Mol. Biochem. Parasitol. 84:1997;137-141
21. Chulay J.D., Haynes J.D., Diggs C.L. Plasmodium falciparum: assessment of in vitro growth by [H-3]-labeled hypoxanthine incorporation. Exp. Parasitol. 55:1983;138-146
22. Peterson D.S., Walliker D., Wellems T.E. Evidence that a point mutation in dihydrofolate reductase-thymidylate synthase confers resistance to pyrimethamine in falciparum malaria. Proc. Natl. Acad. Sci. U.S.A. 85:1988;9114-9118
23. Carreras C.W., Santi D.V. The catalytic mechanism and structure of thymidylate synthase. Ann. Rev. Biochem. 64:1995;721-762
24. Liu J., Schmitz J.C., Lin X.K., et al. Thymidylate synthase as a translational regulator of cellular gene expression. Biochim. Biophys. Acta-Molecular Basis of Disease. 1587:2002;174-182
25. Peters G.J., Backus H.H.J., Freemantle S., et al. Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism. Biochim. Biophys. Acta-Molecular Basis of Disease. 1587:2002;194-205
26. Chu E., Koeller D.M., Johnston P.G., Zinn S., Allegra C.J. Regulation of thymidylate synthase in human colon cancer-cells treated with 5-fluorouracil and interferon-gamma. Mol. Pharmacol. 43:1993;527-533
27. Keyomarsi K., Samet J., Molnar G., Pardee A.B. The thymidylate synthase inhibitor, ICI-D1694, overcomes translational detainment of the enzyme. J. Biol. Chem. 268:1993;15142-15149
28. Liu X.W., Reig B., Nasrallah I.M., Stover P.J. Human cytoplasmic serine hydroxymethyltransferase is an mRNA binding protein. Biochemistry. 39:2000;11523-11531
29. Mosner J., Mummenbrauer T., Bauer C., Sczakiel G., Grosse F., Deppert W. Negative feedback-regulation of wild-type p53 biosynthesis. EMBO J. 14:1995;4442-4449
30. Fu L.N., Minden M.D., Benchimol S. Translational regulation of human p53 gene expression. EMBO J. 15:1996;4392-4401
31. Zhang K., Rathod P.K. Divergent regulation of dihydrofolate reductase between malaria parasite and human host. Science. 296:2002;545-547
32. Ercikan E., Banerjee D., Waltham M., Schnieders B., Scotto K.W., Bertino J.R. Translational regulation of the synthesis of dihydrofolate reductase. Adv. Exp. Med. Biol. 338:1993;537-540