Oral Cancer Cells Differ from Normal Oral Epithelial Cells in Tissuelike Organization and in Response to Lycopene Treatment: An Organotypic Cell Culture Study

Nutrition and Cancer

Volumn 47, Issue 2, 2003, Pages 195-209

  Livny, Orly ^a   Kaplan, Ilana ^b   Reifen, Ram ^a   Polak Charcon, Sylvie ^c   Madar, Zecharia ^a   Schwartz, Betty ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b TEL AVIV UNIVERSITY   (Israel)

^c SHEBA MEDICAL CENTER   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

3 METHYLCHOLANTHRENE; CONNEXIN 43; CYCLINE; CYTOKERATIN; LYCOPENE; VIMENTIN;

ARTICLE; CANCER CELL CULTURE; CANCER INHIBITION; CELL PROLIFERATION; CONTROLLED STUDY; DOSE RESPONSE; ELECTRON MICROSCOPY; GAP JUNCTION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MONOLAYER CULTURE; MOUTH CANCER; MOUTH MUCOSA; WESTERN BLOTTING;

EID: 2342649505     PISSN: 01635581     EISSN: None     Source Type: Journal    
DOI: 10.1207/s15327914nc4702_13     Document Type: Article

References (51)

1. Schroeder K, Koschmieder S, Ottman OG, Hoelzer D, Hauser H, et al.: Coordination of cell growth in cocultures by a genetic proliferation control system. Biotechnol Bioeng 78, 346-352, 2002.
2. 2 oxidative damage in cultured oral epithelial cells: the effect of short-term vitamin C exposure. Anticancer Res 21, 2719-2724, 2001.
3. Oda D, Savard CE, Eng L, Sekijima J, Haigh G, et al.: Reconstituted human oral and esophageal mucosa in culture. In Vitro Cell Dev Biol Anim 34, 46-52, 1998.
4. Aaltonen LM, Wahlstrom T, Rihkanen H, and Vaheri A: A novel method to culture laryngeal human papillomavirus-positive epithelial cells produces a papilloma-type cytology on collagen rafts. Eur J Cancer 34, 1111-1116, 1998.
5. Bickenbach JR and Chism E: Selection and extended growth of marine epidermal stem cells in culture. Exp Cell Res 244, 184-195, 1998.
6. Hietanen S, Syrjanen K, and Syrjanen S: Characterization of keratin and cell cycle protein expression in cell lines from squamous intraepithelial lesions progressing towards a malignant phenotype. Br J Cancer 77, 766-775, 1998.
7. Flores ER, Allen-Hoffmann BL, Lee D, Sattler CA, and Lambert PF: Establishment of papillomavirus type 16 (HPV 16) life cycle in an immortalized human foreskin keratinocyte cell line. Virology 262, 344-354, 1999.
8. Sandalon Z, Fusenig NE, McCutcheon J, Taichman LB, and Garlick JA: Suicide gene therapy for pre-malignant disease: a new strategy for treatment of intraepithelial neoplasia. Gene Ther 8, 232-238, 2001.
9. Oksanen J and Hormia M: An organotypic in vitro model that mimics the dento-epithelial junction. J Periodontol 73, 86-93, 2002.
10. Johnson EJ: The role of lycopene in health and disease. Nutr Clin Care 3, 35-43, 2000.
11. Livny O, Kaplan I, Reifen R, Polak-Charcon S, Madar Z, et al.: Lycopene inhibits proliferation and enhances gap-junction communication of KB-1 human oral tumor cells. J Nutr 132, 3754-3759, 2002.
12. Oda D, Bigler L, Lee P, and Blanton R: HPV immortalization of human oral epithelial cells: a model for carcinogenesis. Exp Cell Res 226, 164-169, 1996.
13. Eicher SA, Clayman GA, Liu TJ, Shillitoe EJ, Storthz KA, et al.: Evaluation of topical gene therapy for head and neck squamous cell carcinoma in an organotypic model. Clin Cancer Res 2,1659-1664,1996.
14. Schwartz B, Benharroch D, Prinslo I, Cagnano E, and Lamprecht SA: Phosphotyrosine, p62 c-myc and p-21 c-Ha-ras proteins in colonic epithelium of normal and dimethylhydrazine-treated rats: an immunohistochemical analysis. Anticancer Res 15, 211-218, 1995.
15. Levy J, Bosin E, Feldman B, Giat Y, Minster A, et al.: Lycopene is a more potent inhibitor of human cancer cell proliferation than either alpha-carotene or beta-carotene. Nutr Cancer 4, 257-266, 1995.
16. Granot I and Dekel N: The ovarian gap junction protein connexin 43: regulation by gonadotropins. Trends Endocrinol Metab 13, 310, 2002.
17. Acevedo P and Bertram JS: Liarozole potentiates the cancer chemopreventive activity of and the up-regulation of gap junctional communication and connexin 43 expression by retinoic acid and beta-carotene in 10T2/2 cells. Carcinogenesis 16, 2215-2222, 1995.
18. Lowry OH, Rosebrough NJ, Farr AL, and Randall RJ: Protein measurement with the folin-phenol reagents. J Biol Chem 193, 265-275, 1951.
19. Pung A, Franke A, Zhang LX, Ippendorf H, Martin HS, et al.: A synthetic C22 carotenoid inhibits carcinogen-induced neoplastic transformation and enhances gap-junctional communication. Carcinogenesis 14, 1001-1005, 1993.
20. Rivedal E, Mikalsen SO, and Sanner T: Morphological transformation and effect on gap junction intercellular communication in Syrian hamster embryo cells as screening tests for carcinogens devoid of mutagenic activity. Toxicol In Vitro 14, 185-192, 2000.
21. DiPaolo JA, Donovan P, and Nelson R: Quantitative studies of in vitro transformation by chemical carcinogens. JNCI 42, 867-874, 1969.
22. Mills PK, Beeson WL, Phillips RL, and Fraser GE: Cohort study of diet, lifestyle, and prostate cancer in Adventist men. Cancer 64, 598-604, 1989.
23. Giovannucci E, Ascherio A, Rimm EB, Stampfer MJ, Colditz GA, et al.: Intake of carotenoids and retinol in relation to risk of prostate cancer. JNCI 87, 1767-1776, 1995.
24. Franceschi S, Bidoli E, La Vecchia C, Talamini R, D'Avanzo B, et al.: Tomatoes and risk of digestive-tract cancers. Int J Cancer 59, 181-184, 1994.
25. Bravo R, Frank R, Blundell PA, and Macdonald-Bravo H: Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta. Nature 326, 515-517, 1987.
26. Kurki P, Ogata K, and Tan EM: Monoclonal antibodies to proliferating cell nuclear antigen (PCNA)/cyclin as probes for proliferating cells by immunofluorescence microscopy and flow cytometry. J Immunol Methods 109, 49-59, 1988.
27. Nahum A, Hirsch K, Danilenko M, Watts CK, Prall OW, et al.: Lycopene inhibition of cell cycle progression in breast and endometrial cancer cells is associated with reduction in cyclin D levels and retention of p27(Kip1) in the cyclin E-cdk2 complexes. Oncogene 20, 3428-3436, 2001.
28. Krutovskikh V, Asamoto M, Takasuka N, Murakoshi M, Nishino H, et al.: Differential dose-dependent effects of alpha-, beta-carotenes and lycopene on gap-junctional intercellular communication in rat liver in vivo. Jpn J Cancer Res 88, 1121-1124, 1997.
29. Stahl W, von Laar J, Martin HD, Emmerich T, and Sies H: Stimulation of gap junctional communication: comparison of acyclo-retinoic acid and lycopene. Arch Biochem Biophys 373, 271-274, 2000.
30. Paul DL: New functions for gap junctions. Curr Opin Cell Biol 7, 665-672, 1995.
31. Kumar NM and Gilula NB: The gap junction communication channel. Cell 84, 381-388, 1996.
32. Yamasaki H and Naus CCG: Role of connexin genes in growth control. Carcinogenesis 17, 1199-1213, 1996.
33. Evans WH and Martin PE: Gap junctions: structure and function (review). Mol Membr Biol 19, 121-136, 2002.
34. Chipman JK: Connexin genes as tumour suppressor genes? Hum Exp Toxicol 14, 539-540, 1995.
35. Loewenstein WR and Rose B: The cell-cell channel in the control of growth. Semin Cell Biol 3, 59-79, 1992.
36. Yamasaki H: Gap junctional intercellular communication and carcinogenesis. Carcinogenesis 11, 1051-1058, 1990.
37. Krutovskikh V and Yamasaki H: The role of gap junctional intercellular communication (GJIC) disorders in experimental and human carcinogenesis. Histol Histopathol 12, 761-768, 1997.
38. Trosko JE and Ruch RJ: Cell-cell communication in carcinogenesis. Front Biosci 3, D208-D236, 1998.
39. Trosko JE and Ruch RJ: Gap junctions as targets for cancer chemoprevention and chemotherapy. Curr Drug Targets 3, 465-482, 2002.
40. Saitoh M, Oyamada M, Oyamada Y, Kaku T, and Mori M: Changes in the expression of gap junction proteins (connexins) in hamster tongue epithelium during wound healing and carcinogenesis. Carcinogenesis 18, 1319-1328, 1997.
41. Zhang LX, Acevedo P, Guo H, and Bertram JS: Up regulation of gap junctional communication and connexin43 gene expression by carotenoids in human dermal fibroblasts but not in human keratinocytes. Mol Carcinogenesis 12, 50-58, 1995.
42. Yamaoka Y, Sawa Y, Ebata N, Ibuki N, Yoshida S, et al.: Double expressions of connexin 43 and 32 in human periodontal ligament fibroblasts. Tissue Cell 32, 328-335, 2000.
43. Valiunas V, Beyer EC, and Brink PR: Cardiac gap junction channels show quantitative differences in selectivity. Circ Res 91, 104-111, 2002.
44. Yoshimura T, Satake M, and Kobayashi T: Connexin43 is another gap junction protein in the peripheral nervous system. J Neurochem 67, 1252-1258, 1996.
45. King TJ, Fukushima LH, Yasui Y, Lampe PD, and Bertram JS: Inducible expression of the gap junction protein connexin 43 decreases the neoplastic potential of HT-1080 human fibrosarcoma cells in vitro and in vivo. Mol Carcinogenesis 35, 29-41, 2002.
46. Heppner FL, Skutella T, Hailer NP, Haas D, and Nitsch R: Activated microglial cells migrate towards sites of excitotoxic neuronal injury inside organotypic hippocampal slice cultures. Eur J Neurosci 10, 3284-3290, 1998.
47. Plenz D and Aertsen A: Neuronal dynamics in cortex-striatum co-cultures. II. Spatio-temporal characteristics of neuronal activity. Neuroscience 70, 893-924, 1996.
48. Goto Y, Noguchi Y, Nomura A, Sakamoto T, Ishii Y, et al.: In vitro reconstitution of the tracheal epithelium. Am J Respir Cell Mol Biol 20, 312-318, 1999.
49. Jacks T and Weinberg RA: Taking the study of cancer cell survival to a new dimension. Cell 111, 923-925, 2002.
50. Dam N and Bouquot A: Oral and Maxillofacial Pathology, Philadelphia, PA: WB Saunders, 1995.
51. Bhuvaneswari V, Velmurugan B, Balasenthil S, Ramachandran CR, and Nagini S: Chemopreventive efficacy of lycopene on 7,12 dimethylbenz (a) anthracene-induced hamster buccal pouch carcinogenesis. Fitoterapia 72, 865-874, 2001.