Ubiquinone is not required for proton conductance by uncoupling protein 1 in yeast mitochondria

Biochemical Journal

Volumn 379, Issue 2, 2004, Pages 309-315

  Esteves, Telma C ^a   Echtay, Karim S ^a,c   Jonassen, Tanya ^b   Clarke, Catherine F ^b   Brand, Martin D ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF BALAMAND   (Lebanon)

Author keywords

Mitochondria; Proton conductance; Retinoic acid; Ubiquinone (coenzyme Q); UCP1 (uncoupling protein 1); Yeast

Indexed keywords

CELLS; FATTY ACIDS; MUTAGENESIS; PROTEINS; SENSITIVITY ANALYSIS; YEAST;

MUTANT STRAINS; PROTON CONDUCTANCE; PROTON-TRANSPORT; UNCOUPLING PROTEIN;

PROTONS;

GUANOSINE DIPHOSPHATE; UBIQUINONE; UNCOUPLING PROTEIN 1;

ARTICLE; CELL ISOLATION; CONTROLLED STUDY; CROSS COUPLING REACTION; ENZYME SYNTHESIS; GENE; GENE ACTIVITY; MITOCHONDRIAL RESPIRATION; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTON TRANSPORT; SACCHAROMYCES CEREVISIAE; WILD TYPE; YEAST;

CARRIER PROTEINS; ION CHANNELS; ION TRANSPORT; MEMBRANE PROTEINS; MITOCHONDRIA; MITOCHONDRIAL PROTEINS; MUTATION; PROTONS; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC; UBIQUINONE;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 2342597745     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20031682     Document Type: Article

References (34)

1. Nicholls, D. G. and Rial, E. (1999) A history of the first uncoupling protein, UCP1. J. Bioenerg. Biomembr. 31, 399-406
2. Arechaga, I., Ledesma, A. and Rial, E. (2001) The mitochondrial uncoupling protein UCP1: a gated pore. IUBMB Life 52, 165-173
3. Klingenberg, M. and Echtay, K. S. (2001) Uncoupling proteins: the issues from a biochemist's point of view. Biochim. Biophys. Acta 1504, 128-143
4. Lin, C. S. and Klingenberg, M. (1980) Isolation of the uncoupling protein from brown adipose tissue mitochondria. FEBS Lett. 113, 299-303
5. Jaburek, M., Varecha, M., Gimeno, R. E., Dembski, M., Jezek, P., Zhang, M., Burn, P., Tartaglia, L. A. and Garlid, K. D. (1999) Transport function and regulation of mitochondrial uncoupling proteins 2 and 3. J. Biol. Chem. 274, 26003-26007
6. + transporters and highly nucleotide sensitive when activated by coenzyme Q (ubiquinone). Proc. Natl. Acad. Sci. U.S.A. 98, 1416-1421
7. Jekabsons, M. B., Echtay, K. S. and Brand, M. D. (2002) Nucleotide binding to human uncoupling protein-2 refolded from bacterial inclusion bodies. Biochem. J. 366, 565-571
8. Echtay, K. S., Winkler, E. and Klingenberg, M. (2000) Coenzyme Q is an obligatory cofactor for uncoupling protein function. Nature (London) 408, 609-613
9. Tomás, P., Ledesma, A. and Rial, E. (2002) Photoaffinity labeling of the uncoupling protein UCP1 with retinoic acid: ubiquinone favors binding. FEBS Lett. 526, 63-65
10. Rial, E., Gonzalez-Barroso, M., Fleury, C., Iturrizaga, S., Sanchis, D., Jimenez-Jimenez, J., Ricquier, D., Goubern, M. and Bouillaud, F. (1999) Retinoids activate proton transport by the uncoupling proteins UCP1 and UCP2. EMBO J. 18, 5827-5833
11. Klingenberg, M. (2001) Uncoupling proteins - how do they work and how are they regulated. IUBMB Life 52, 175-179
12. Tzagoloff, A. and Dieckmann, C. L. (1990) PET genes of Saccharomyces cerevisiae. Microbiol. Rev. 54, 211-225
13. Jonassen, T. and Clarke, C. F. (2000) Genetic analysis of coenzyme Q biosynthesis. In Coenzyme Q: Molecular mechanisms in health and disease (Kagan, V. E. and Quinn, P. J., eds.), pp. 185-208, CRC Press, Boca Raton, FL
14. Hsu, A. Y., Do, T. Q., Lee, P. T. and Clarke, C. F. (2000) Genetic evidence for a multi-subunit complex in the O-methyltransferase steps of coenzyme Q biosynthesis. Biochim. Biophys. Acta 1484, 287-297
15. Poon, W. W., Barkovich, R. J., Hsu, A. Y., Frankel, A., Lee, P. T., Shepherd, J. N., Myles, D. C. and Clarke, C. F. (1999) Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis. J. Biol. Chem. 274, 21665-21672
16. Poon, W. W., Marbois, B. N., Faull, K. F. and Clarke, C. F. (1995) 3-Hexaprenyl-4-hydroxybenzoic acid forms a predominant intermediate pool in ubiquinone biosynthesis in Saccharomyces cerevisiae. Arch. Biochem. Biophys. 320, 305-314
17. Poon, W. W., Do, T. Q., Marbois, B. N. and Clarke, C. F. (1997) Sensitivity to treatment with polyunsaturated fatty acids is a general characteristic of the ubiquinone-deficient yeast coq mutants. Mol. Aspects Med. 18 (Suppl. 1), S121-S127
18. Ashby, M. N. and Edwards, P. A. (1990) Elucidation of the deficiency in two yeast coenzyme Q mutants. Characterization of the structural gene encoding hexaprenyl pyrophosphate synthetase. J. Biol. Chem. 265, 13157-13164
19. Proft, M., Kotter, P., Hedges, D., Bojunga, N. and Entian, K. D. (1995) CAT5, a new gene necessary for derepression of gluconeogenic enzymes in Saccharomyces cerevisiae. EMBO J. 14, 6116-6126
20. Harper, J. A. (2002) Expression and characterisation of the uncoupling proteins in yeast. Ph.D. Thesis, University of Cambridge, Cambridge, U.K. pp. 75-76
21. Stuart, J. A., Harper, J. A., Brindle, K. M., Jekabsons, M. B. and Brand, M. D. (2001) A mitochondrial uncoupling artifact can be caused by expression of uncoupling protein 1 in yeast. Biochem. J. 356, 779-789
22. 2 solubility in aqueous media determined by a kinetic method. Anal. Biochem. 145, 406-418
23. 8 to mitochondria. J. Biol. Chem. 277, 45020-45027
24. Brand, M. D. (1995) Measurement of mitochondrial protonmotive force. In Bioenergetics - A Practical Approach (Brown, G. C. and Cooper, C. E., eds.), pp. 39-62, IRL Press, Oxford
25. 1 complex stability in yeast coq mutants. J. Biol. Chem. 277, 10973-10981
26. Bathgate, B., Freebairn, E. M., Greenland, A. J. and Reid, G. A. (1992) Functional expression of the rat brown adipose tissue uncoupling protein in Saccharomyces cerevisiae. Mol. Microbiol. 6, 363-370
27. Arechaga, I., Raimbault, S., Prieto, S., Levi-Meyrueis, C., Zaragoza, P., Miroux, B., Ricquier, D., Bouillaud, F. and Rial, E. (1993) Cysteine residues are not essential for uncoupling protein function. Biochem. J. 296, 693-700
28. Echtay, K. S. and Brand, M. D. (2001) Coenzyme Q induces GDP-sensitive proton conductance in kidney mitochondria. Biochem. Soc. Trans. 29, 763-768
29. Echtay, K. S., Roussel, D., St-Pierre, J., Jekabsons, M. B., Cadenas, S., Stuart, J. A., Roebuck, S. J., Morrison, A., Pickering, S., Clapham, J. C. and Brand, M. D. (2002) Superoxide activates mitochondrial uncoupling proteins. Nature (London) 415, 96-99
30. Echtay, K. S., Esteves, T. C., Pakay, J. L., Jekabsons, M. B., Lambert, A. J., Portero-Otin, M., Pamplona, R., Vidal-Puig, A. J., Wang, S., Roebuck, S. J. et al. (2003) A signalling role for 4-hydroxy-2-nonenal in regulation of mitochondrial uncoupling. EMBO J. 22, 4103-4110
31. Murphy, M. P., Echtay, K. S., Blaikie, F. H., Asin-Cayuela, J., Cochemé, H. M., Green, K., Buckingham, J. A., Taylor, E. R., Hurrell, F., Hughes, G. et al. (2003) Superoxide activates uncoupling proteins by generating carbon-centered radicals and initiating lipid peroxidation: studies using a mitochondria-targeted spin trap derived from α-phenyl-N-tert-butylnitrone. J. Biol. Chem. 278, 48534-48545
32. Brand, M. D., Buckingham, J. A., Esteves, T. C., Green, K., Lambert, A. J., Miwa, S., Murphy, M. P., Pakay, J. L., Talbot, D. A. and Echtay, K. S. (2004) Mitochondrial superoxide and ageing. Biochem. Soc. Symp. 71, in the press
33. Chomiki, N., Voss, J. C. and Warden, C. H. (2001) Structure-function relationships in UCP1, UCP2 and chimeras: EPR analysis and retinoic acid activation of UCP2. Eur. J. Biochem. 268, 903-913
34. 10. J. Biol. Chem. 278, 25825-25831