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Volumn 45, Issue 21, 2004, Pages 4163-4166

Synthesis of a novel dioxan sialic acid analog

Author keywords

Sialic acid; Sialidase; Sialyltransferase

Indexed keywords

DIOXANE DERIVATIVE; MANNOSE; SIALIC ACID DERIVATIVE;

EID: 2342588824     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tetlet.2004.03.135     Document Type: Article
Times cited : (2)

References (47)
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    • 13C NMR, and/or mass spectral data, in full agreement with their assigned structures and stereochemistries
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    • note
    • 2O)
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    • b Inhibitors were added to a reaction mixture containing 2 mg/mL asialofetuin as acceptor, 0.5 mU/mL purified rat liver α-2,6-sialyltransferase (Boehringer M. 981 583) and 1 μM CMP-9-fluoresceinyl-Neu5Ac. After 1 h incubation at 37°C, fluorescence of the reaction product was read at 530 nm.
    • b Inhibitors were added to a reaction mixture containing 2 mg/mL asialofetuin as acceptor, 0.5 mU/mL purified rat liver α-2, 6-sialyltransferase (Boehringer M. 981 583) and 1 μM CMP-9-fluoresceinyl-Neu5Ac. After 1 h incubation at 37°C, fluorescence of the reaction product was read at 530 nm. Laroy W., Maras M., Fiers W., Contreras R. Anal. Biochem. 249:1997;108-111
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    • Laroy, W.1    Maras, M.2    Fiers, W.3    Contreras, R.4
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    • note
    • The ratio of compounds 5 and 6 was determined on the amount of each compound isolated by flash chromatography (silica gel)
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    • note
    • 13CNMR signal areas, a 1/9 ratio of compounds 5/6 in favor of the axial conformation
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    • note
    • A solution of the amine (1 equiv) ethyl glycoxylate (1.2 equiv) and paradisulfonic acid (0.01 equiv) was refluxed in toluene until complexion of the reaction


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