Low molecular weight protamine as an efficient and nontoxic gene carrier: In vitro study

Journal of Gene Medicine

Volumn 5, Issue 8, 2003, Pages 700-711

  Park, Yoon Jeong ^a   Liang, Jun Feng ^a   Ko, Kyung Soo ^b   Kim, Sung Wan ^c   Yang, Victor C ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

^b Inje University   (South Korea)

^c UNIVERSITY OF UTAH   (United States)

Author keywords

Gene therapy; Gene transfer; Low molecular weight protamine; TAT peptide; Transcellular localization

Indexed keywords

DEOXYRIBONUCLEASE I; NAKED DNA; PLASMID DNA; POLYETHYLENEIMINE; PROTAMINE; TRANSACTIVATOR PROTEIN; DNA; PEPTIDE;

ARTICLE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOPLASM; CYTOTOXICITY; DNA TRANSFER; ENZYME ASSAY; FLOW CYTOMETRY; GENE TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR SIZE; MOLECULAR WEIGHT; NONVIRAL GENE DELIVERY SYSTEM; NONVIRAL GENE THERAPY; PRIORITY JOURNAL; PROTECTION; ZETA POTENTIAL; AMINO ACID SEQUENCE; ANIMAL; CELL LINE; CELL SURVIVAL; CHEMISTRY; GENE THERAPY; GENETICS; MACROMOLECULE; METABOLISM; PLASMID;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CELL SURVIVAL; DNA; GENE PRODUCTS, TAT; GENE THERAPY; GENE TRANSFER TECHNIQUES; HUMANS; MACROMOLECULAR SUBSTANCES; MOLECULAR WEIGHT; PEPTIDES; PLASMIDS; PROTAMINES; TRANSFECTION;

EID: 2342476860     PISSN: 1099498X     EISSN: None     Source Type: Journal    
DOI: 10.1002/jgm.402     Document Type: Article

References (32)

1. Maheshwari A, Mahato RI, McGregor J, et al. Soluble biodegradable polymer-based cytokine gene delivery for cancer treatment. Mol Ther 2000; 2: 121-130.
2. Lee M, Han S, Ko KS, Kim SW. Cell type specific and glucose responsive expression of interleukin-4 by using insulin promoter and water soluble lipopolymer. J Control Release 2001; 75: 421-429.
3. Kabanov AV, Kabanov VA. DNA complexes with polycations for the delivery of genetic material into cells. Bioconjugate Chem 1995; 6: 7-20.
4. Gilboa E, Eglitis MA, Kantoff PW, Anderson FW. Transfer and expression of cloned genes using retroviral vectors. Biotechniques 1986; 4:504-512.
5. Mahato RI, Rolland A, Tomlison E. Cationic lipid-based gene delivery systems: pharmaceutical perspectives. Pharm Res 1997; 14: 853-859.
6. Blessing T, Remy JS, Behr JP. Monomolecular collapse of plasmid DNA into stable virus-like particles. Proc Natl Acad Sci USA 1998; 95: 1427-1431.
7. Rosenfeld MA, Siegfried W, Yoshimura K, et al. Adenovirus-mediated transfer of a recombinant al-antitrypsin gene to the lung epithelium in vivo. Science 1991; 252: 431-434.
8. Miller DG, Adam MA, Miller AD. Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection. Mol Cell Biol 1990; 10: 4239-4242.
9. Sakurai F, Inoue R, Nishino Y, et al. Effect of DNA/liposome mixing ratio on the physicochemical characteristics, cellular uptake and intracellular trafficking of plasmid DNA/cationic liposome complexes and subsequent gene expression. J Control Release 2000; 66: 255-269.
10. Caplen NJ, Alton EWFW, Middleton PG, et al. Liposome-mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis. Nat Med 1995; 1: 39-46.
11. Sorgi FL, Bhattacharya S, Huang L. Protamine sulfate enhances lipid-mediated gene transfer. Gene Ther 1997; 4: 961-968.
12. Wadhwa MS, Collard WT, Adami RC, McKenzie DL, Rice KG. Peptide-mediated gene delivery: influence of peptide structure on gene expression. Bioconjugate Chem 1997; 8: 81-88.
13. Zauner W, Kichler A, Schmidt W, Sinski A, Wagner E. Glycerol enhancement of ligand-polylysine/DNA transfection. Biotechniques 1996; 20: 905-913.
14. Midoux P, Monsigny M. Efficient gene transfer by hystidylated polylysine/pDNA complexes. Bioconjugate Chem 1999; 10: 406-411.
15. Abdallah B, Hassan A, Benoist C, Goula D, Behr JP, Demeneix BA. A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: polyethyleneimine. Hum Gene Ther 1996; 7: 1947-1954.
16. Wightman L, Kircheis R, Rossler V, et al. Different behavior of branched and linear polyethyleneimine for gene delivery in vitro and in vivo. J Gene Med 2001; 3: 362-372.
17. Morgan DML, Laavin VL, Pearson JD. Biochemical characterization of polycation-induced cytotoxicity to human vascular endothelial cells. J Cell Sci 1987; 94: 553-560.
18. Chang LC, Lee HF, Yang Z, Yang VC. Low molecular weight protamine (LMWP) as nontoxic heparin/low molecular weight heparin antidote. I. Preparation and characterization. AAPS PharmSci 2001;3(2): article 17.
19. Chang LC, Liang JF, Lee HF, Lee LM, Yang VC. Low molecular weight protamine (LMWP) as nontoxic heparin/low molecular weight heparin antidote. II. In vitro evaluation of efficacy and toxicity. AAPS PharmSci 2001; 3(2): article 18.
20. Chang LC, Wrobleski S, Wakefield TW, Lee LM, Yang VC. Low molecular weight protamine as nontoxic heparin/low molecular weight heparin antidote. III. Preliminary in vivo evaluation of efficacy and toxicity using a canine model. AAPS PharmSci 2001; 3(2): article 19.
21. Casellas P, Ravel S, Bourrie BJ, et al. T-lymphocyte killing by T101-ricin A-chain immunotoxin: pH-dependent potentiation with lysosomotropic amines. Blood 1988; 72: 1197-1201.
22. Vives E, Brodin P, Lebleu B. A truncated HIV-1 TAT protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. J Biol Chem 1997; 272: 16010-16017.
23. Kip1 induces cell migration. Nat Med 1998; 4: 1449-1452.
24. Schwarze SR, Dowdy SF. In vivo protein transduction: intracellular delivery of biologically active proteins, compounds and DNA. Trends Pharmacol Sci 2000; 21: 45-48.
25. Schwarze SR, Ho A, Vocero-Akbani A, Dowdy SF. In vivo protein transduction: delivery of a biologically active protein into the mouse. Science 1999; 285: 1569-1572.
26. Lampela P, Raisanen J, Mannisto PT, Yla-Herttuala S, Raasmaja A. The use of low-molecular-weight PEIs as gene carriers in the monkey fibroblastoma and rabbit smooth muscle cell cultures. J Gene Med 2002; 4: 205-214.
27. Tung CH, Mueller S, Weissleder R. Novel branching membrane translocational peptide as gene delivery vector. Bioorganic Med Chem 2002; 10(11): 3609-3614.
28. Benimetskaya L, Guzzo-Pernell N, Liu ST, Lai JCH, Miller P, Stein CA. Protamine-fragment peptides fused to an SV40 nuclear localization signal deliver oligonucleotides that produce antisense effects in prostate and bladder carcinoma cells. Bioconjugate Chem 2002; 13: 177-187.
29. Astriab-Fisher A, Sergueev D, Fisher M, Shaw BR, Juliano RL. Conjugates of antisense oligonucleotides with the Tat and Antennapedia cell-penetrating peptides: effects on cellular uptake, binding to target sequences, and biologic actions. Pharm Res 2002; 19: 744-754.
30. Wender PA, Mitchell DJ, Pattabiraman K, Pelkey ET, Steinman L, Rothbard JB. The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake: peptoid molecular transporters. Proc Natl Acad Sci USA 2000; 97: 13003-13008.
31. Futaki S, Suzuki T, Ohashi W, et al. Arginine-rich peptides: an abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery. J Biol Chem 2001; 276: 5836-5840.
32. Morris MC, Depollier J, Mery J, Heitz F, Divita G. A peptide carrier for the delivery of biologically active proteins into mammalian cells. Nat Biotechnol 2001; 19: 1173-1176.