Refolding of lysozyme at high concentration in batch and fed-batch operation

Korean Journal of Chemical Engineering

Volumn 19, Issue 5, 2002, Pages 871-875

  Gao, Yong Gui ^a   Guan, Yi Xin ^a   Yao, Shan Jing ^a   Cho, Man Gi ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b Dongseo University   (South Korea)

Author keywords

Dilution; Disulfide bond; Fed batch operation; Lysozyme; Protein refolding

Indexed keywords

EID: 23044533315     PISSN: 02561115     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02706982     Document Type: Article

References (14)

1. Batas, B. and Chaudhuri, J. B., "Protein Refolding at High Concentration Using Size-exclusion Chromatography," Biotech, and Bioeng., 50,16(1996).
2. Brandford, M. M, "A Rapid and Sensitive Method for the Quantitation of Protein Utilizing the Principles of Protein-dye Binding," Anal. Biochem., 72,248 (1976).
3. Cardamone, M., Puri, N. K. and Brandon, M. R., "Comparing the Refolding and Reoxidation of Recombinant Porcine Growth Hormone from a Urea Denatured State and from Escherichia coli In- clusion Bodies," Biochemistry, 34,5773 (1995).
4. Eliana, D. B. C, "Protein Refolding for Industrial Processes," Current Opinion in Biotechnology, 12,202 (2001).
5. Fahey, E. M. and Chaudhuri, J. B., "Refolding of Low Molecular Weight Urokinase Plasminogen Activator by Dilution and Size Exclusion Chromatography- a Comparative Study," Separation Science and Technology, 35, 1743 (2000).
6. Katoh, S., Sezai, Y, Yamaguchi, T., Katoh Y, Yagi, H. and Nohara, D., "Refolding of Enzyme in a Fed-batch Operation," Process Biochem.,35,297 (1997).
7. Kim, Y. C. and Myerson, A. S., "Diffiisivity of Protein in Aqueous Solutions," Korean J. Chem. Eng., 13,288 (1996).
8. Kuboi, R., Morita, S., Ota, H. and Umakoshi, H., "Protein Refolding Using Stimuli-responsive Polymer-modified Aqueous Two-phase Systems," Journal of Chromatography B., 743,215 (2000).
9. Maeda, Y, Yamada, H., Ueda, T. and Imoto, T, "Effect of Additives on the Renaturation of Reduced Lysozyme in the Presence of 4 M Urea," Protein Engineering, 9,461 (1996).
10. Martin, W., Raimond, B. G. R., Gitay, K., Sean, M. S., Maria, L. R., MichaL H., Piet, G., Israel, S., Jan, K. and Joel, L. S., "Specific Chemical and Structural Damage to Proteins Produced by Synchrotron Radiation," Pmc. Natl. Acad. Sci. USA, 97(2), 623 (2000).
11. Müller, C. and Rinas, U., "Renaturation of Heterodimeric Platelet- derived Growth Factor from Inclusion Bodies of Recombinant Escherichia coli Using Size-exclusion Chromatography," J. Chro- matograplxy A., 855,203 (1999).
12. Rozema, D. and Gellman, S. H., "Artificial Chaperone-assisted Refolding of Denatured-reduced Lysozyme: Modulation of the Competition Between Renaturation and Aggregation," Biochem., 35,15760 (1996).
13. Stellmach, B. and Qian, J. Y, "Enzyme Measurement," China Light Industry Press (1992).
14. Terashima, T., Suzuki, K. and Katoh, S., "Effective Refolding of Fully Reduced Lysozyme with a Flow-type Reactof," Process Biochem., 31,341 (1996).