Correlation between crystal violet dissolution assay and manual colony counting on the In Vitro effects of Hsp90-inhibitors

Journal of Experimental Therapeutics and Oncology

Volumn 5, Issue 1, 2005, Pages 9-13

  Ochel, Hans Joachim ^a  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

Author keywords

Bivariate correlation; Colony number; Crystal violet

Indexed keywords

17 ALLYLAMINO 17 DEMETHOXYGELDANAMYCIN; GELDANAMYCIN; HEAT SHOCK PROTEIN 90 INHIBITOR; PROTEIN INHIBITOR; RADICICOL; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; CARCINOMA CELL; CELL SURVIVAL; COLONY FORMATION; CRYSTAL VIOLET DISSOLUTION ASSAY; CYTOTOXICITY; HUMAN; HUMAN CELL; IN VITRO STUDY; INTERMETHOD COMPARISON; IONIZING RADIATION; MANUAL COLONY COUNTING; PRIORITY JOURNAL; PROSTATE CARCINOMA; QUANTITATIVE ANALYSIS; RADIATION DOSE; RADIATION EXPOSURE; RADIOBIOLOGY; REPRODUCIBILITY;

CELL LINE, TUMOR; COLORING AGENTS; DRUG SCREENING ASSAYS, ANTITUMOR; GENTIAN VIOLET; HSP90 HEAT-SHOCK PROTEINS; HUMANS; MALE; OBSERVER VARIATION; PROSTATIC NEOPLASMS; RADIATION DOSAGE; REPRODUCIBILITY OF RESULTS; SOLUBILITY; TUMOR STEM CELL ASSAY;

EID: 23044512519     PISSN: 13594117     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Gillies RJ, Didier N, Denton M. Determination of cell number in monolayer cultures. Anal Biochem 159:109-113, 1986.
2. Flick DA, Gifford GE. Comparison of in vitro cell cytotoxic assays for tumor necrosis factor. J Immunol Meth 68:167-175, 1984.
3. Yang YY, Olofsson M, Kopf I, Hultborn R. Growth and clonogenic assays compared for irradiated MCF-7 and Colo-205 cell lines. Anticancer Res 18:53-59, 1998
4. Streffer JR, Schuster M, Pohl U, Belka C, Dichgans J, Bamberg M, Weller M. Irradiation induced clonogenic cell death of human malignant glioma cells does not require CD95/CD95L interactions. Anticancer Res 19:5265-5269, 1999.
5. Ochel H-J, Gademann G. Heat-Shock Protein 90: Potential involvement in the pathogenesis of malignancy and pharmacological intervention. Onkologie 25:466-473, 2002.
6. Schulte TW, Neckers LM:. The benzoquinone ansamycin 17-allylamino-17- demethoxygeldanamycin binds to hsp90 and shares important biologic activities with geldanamycin. Cancer Chemother Pharmacol 42:273-279, 1998.
7. Schulte TW, Akinaga S, Soga S, Sullivan W, Stensgard D, Toft D, Neckers LM. Antibiotic radicicol binds to the N-terminal domain of Hsp90 and shares important biologic activities with geldanamycin. Cell Stress Chaperones 3:100-108, 1998.
8. Grenert JP, Sullivan WP, Fadden P, Haystead TAJ, Clark J, Mimnaugh E, Krutzsch H, Ochel HJ, Schulte TW, Sausville E, Neckers LM, Toft DO. The amino-terminal domain of heat shock protein 90 (hsp90) that binds geldanamycin is an ATP/ADP switch domain that regulates hsp90 conformation. J Biol Chem 272:23843-23850, 1997.
9. Kueng W, Silber E, Eppenberger U: Quantification of cells cultured on 96-well plates. Anal Biochem 182:16-19, 1989