Probing PrPSc structure using chemical cross-linking and mass spectrometry: Evidence of the proximity of Gly90 amino termini in the PrP 27-30 aggregate

Biochemistry

Volumn 44, Issue 30, 2005, Pages 10100-10109

  Onisko, Bruce ^a   Fernández, Esteban Guitián ^b   Freire, María Louro ^c   Schwarz, Anja ^d   Baier, Michael ^d   Camiña, Félix ^b   García, Javier Rodríguez ^b   Villamarín, Santiago Rodríguez Segade ^b   Requena, Jesús R ^c  

^a WESTERN REGIONAL RESEARCH CENTER   (United States)

^b UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^c UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^d ROBERT KOCH INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CROSSLINKING; MASS SPECTROMETRY; OLIGOMERS; REFUSE DIGESTION;

CHEMICAL CROSS LINKING; ELECTRON CRYSTALLOGRAPHIC DATA; ELUCIDATION; PROTEOLYTIC DIGESTION;

MOLECULAR STRUCTURE;

BIS(SULFOSUCCINIMIDYL)SUBERATE; DIMER; GLYCINE; OLIGOMER; PRION PROTEIN; REAGENT; SUCCINIMIDE DERIVATIVE; UNCLASSIFIED DRUG; CROSS LINKING REAGENT; LYSINE; LYSYL ENDOPEPTIDASE; PEPTIDE FRAGMENT; PROTEINASE K; SERINE PROTEINASE; TRYPSIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CROSS LINKING; CRYSTALLOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SCRAPIE; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; DIMERIZATION; ELECTROSPRAY MASS SPECTROMETRY; HAMSTER; HYDROLYSIS; METABOLISM; NANOTECHNOLOGY; SYRIAN HAMSTER;

MESOCRICETUS AURATUS;

AMINO ACID SEQUENCE; ANIMALS; CRICETINAE; CROSS-LINKING REAGENTS; DIMERIZATION; ENDOPEPTIDASE K; GLYCINE; HYDROLYSIS; LYSINE; MESOCRICETUS; NANOTECHNOLOGY; PEPTIDE FRAGMENTS; PRP 27-30 PROTEIN; SERINE ENDOPEPTIDASES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUCCINIMIDES; TRYPSIN;

EID: 23044458520     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0501582     Document Type: Article

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