Stable-isotope dilution liquid chromatography-electrospray injection tandem mass spectrometry method for fast, selective measurement of S-adenosylmethionine and S-adenosylhomocysteine in plasma

Clinical Chemistry

Volumn 51, Issue 8, 2005, Pages 1487-1492

  Gellekink, Henkjan ^a   Van Oppenraaij Emmerzaal, Dinny ^a   Van Rooij, Arno ^a   Struys, Eduard A ^b   Heijer, Martin Den ^a   Blom, Henk J ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^b VU UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ACETIC ACID; BENZENEBORONIC ACID; S ADENOSYLHOMOCYSTEINE; S ADENOSYLMETHIONINE;

ACIDIFICATION; AMINO ACID BLOOD LEVEL; ARTICLE; CONTROLLED STUDY; DEGRADATION KINETICS; DIAGNOSTIC PROCEDURE; DRUG POTENCY; EARLY DIAGNOSIS; ELECTROSPRAY INJECTION TANDEM MASS SPECTROMETRY; ELECTROSPRAY MASS SPECTROMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; ISOTOPE DILUTION ASSAY; LIQUID CHROMATOGRAPHY; METHYLATION; MOLECULAR STABILITY; PRODUCT RECOVERY; QUANTITATIVE DIAGNOSIS; SENSITIVITY ANALYSIS; SEPARATION TECHNIQUE; SOLID PHASE EXTRACTION; STANDARD; TRANSMETHYLATION;

CHROMATOGRAPHY, LIQUID; DEUTERIUM; HUMANS; RADIOISOTOPE DILUTION TECHNIQUE; REFERENCE VALUES; S-ADENOSYLHOMOCYSTEINE; S-ADENOSYLMETHIONINE; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 23044449221     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2004.046995     Document Type: Article

References (17)

1. Zhu BT. On the mechanism of homocysteine pathophysiology and pathogenesis: a unifying hypothesis. Histol Histopathol 2002;17:1283-91.
2. Rosenquist TH, Finnell RH. Genes, folate and homocysteine in embryonic development. Proc Nutr Soc 2001;60:53-61.
3. Loehrer FM, Tschopl M, Angst CP, Litynski P, Jager K, Fowler B, et al. Disturbed ratio of erythrocyte and plasma S-adenosylmethionine/S- adenosylhomocysteine in peripheral arterial occlusive disease. Atherosclerosis 2001;154:147-54.
4. Castro R, Rivera I, Struys EA, Jansen EE, Ravasco P, Camilo ME, et al. Increased homocysteine and S-adenosylhomocysteine concentrations and DNA hypomethylation in vascular disease. Clin Chem 2003;49:1292-6.
5. Yi P, Melnyk S, Pogribna M, Pogribny IP, Hine RJ, James SJ. Increase in plasma homocysteine associated with parallel increases in plasma S-adenosylhomocysteine and lymphocyte DNA hypomethylation. J Biol Chem 2000;275:29318-23.
6. James SJ, Melnyk S, Pogribna M, Pogribny IP, Caudill MA. Elevation in S-adenosylhomocysteine and DNA hypomethylation: potential epigenetic mechanism for homocysteine-related pathology. J Nutr 2002;132:2361S-6S.
7. Kerins DM, Koury MJ, Capdevila A, Rana S, Wagner C. Plasma S-adenosylhomocysteine is a more sensitive indicator of cardiovascular disease than plasma homocysteine. Am J Clin Nutr 2001;74:723-9.
8. te Poele-Pothoff MT, van den Berg M, Franken DG, Boers GH, Jakobs C, de Kroon IF, et al. Three different methods for the determination of total homocysteine in plasma. Ann Clin Biochem 1995;32:218-20.
9. Struys EA, Jansen EE, de Meer K, Jakobs C. Determination of S-adenosylmethionine and S-adenosylhomocysteine in plasma and cerebrospinal fluid by stable-isotope dilution tandem mass spectrometry. Clin Chem 2000;46:1650-6.
10. Delabar U, Kloor D, Luippold G, Muhlbauer B. Simultaneous determination of adenosine, S-adenosylhomocysteine and S-adenosylmethionine in biological samples using solid-phase extraction and high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl 1999;724:231-8.
11. Stabler SP, Allen RH. Quantification of serum and urinary S-adenosylmethionine and S-adenosylhomocysteine by stable-isotope-dilution liquid chromatography-mass spectrometry. Clin Chem 2004;50:365-72.
12. Willems HP, Bos GM, Gerrits WB, den Heijer M, Vloet S, Blom HJ. Acidic citrate stabilizes blood samples for assay of total homocysteine. Clin Chem 1998;44:342-5.
13. Mattson MP. Methylation and acetylation in nervous system development and neurodegenerative disorders. Ageing Res Rev 2003;2:329-42.
14. Capdevila A, Wagner C. Measurement of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent isoindoles. Anal Biochem 1998;264:180-4.
15. Loehrer FM, Haefeli WE, Angst CP, Browne G, Frick G, Fowler B. Effect of methionine loading on 5-methyltetrahydrofolate, S-adenosylmethionine and S-adenosylhomocysteine in plasma of healthy humans. Clin Sci (Lond) 1996;91:79-86.
16. Loehrer FM, Angst CP, Brunner FP, Haefeli WE, Fowler B. Evidence for disturbed S-adenosylmethionine:S-adenosylhomocysteine ratio in patients with end-stage renal failure: a cause for disturbed methylation reactions? Nephrol Dial Transplant 1998;13:656-61.
17. Melnyk S, Pogribna M, Pogribny IP, Yi P, James SJ. Measurement of plasma and intracellular S-adenosylmethionine and S-adenosylhomocysteine utilizing coulometric electrochemical detection: alterations with plasma homocysteine and pyridoxal 5′-phosphate concentrations. Clin Chem 2000;46:265-72.