Increased homocysteine and S-adenosylhomocysteine concentrations and DNA hypomethylation in vascular disease

Clinical Chemistry

Volumn 49, Issue 8, 2003, Pages 1292-1296

  Castro, Rita ^a   Rivera, Isabel ^a   Struys, Eduard A ^b   Jansen, Erwin E W ^b   Ravasco, Paula ^c   Camilo, Maria Ermelinda ^c   Blom, Henk J ^d   Jakobs, Cornelis ^b   De Almeida, Isabel Tavares ^a  

^a RESEARCH INSTITUTE FOR MEDICINES IMED ULISBOA   (Portugal)

^b VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^c INSTITUTO DE MEDICINA MOLECULAR   (Portugal)

^d RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; HOMOCYSTEINE; METHYLTRANSFERASE; S ADENOSYLHOMOCYSTEINE;

ADULT; AMINO ACID BLOOD LEVEL; AMINO ACID METABOLISM; ARTICLE; CARDIOVASCULAR RISK; CLINICAL ARTICLE; CONTROLLED STUDY; DNA METHYLATION; GENOMICS; HUMAN; HUMAN CELL; LEUKOCYTE; MALE; PATHOGENESIS; VASCULAR DISEASE;

CARDIOVASCULAR DISEASES; DNA METHYLATION; HOMOCYSTEINE; HUMANS; MALE; MIDDLE AGED; S-ADENOSYLHOMOCYSTEINE;

EID: 0041866794     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/49.8.1292     Document Type: Article

References (20)

1. Friso S, Choi SW, Girelli D, Mason JB, Dolnikowski GG, Bagley PJ, et al. A common mutation in the 5,10-methylenetetrahydrofolate reductase gene affects genomic DNA methylation through an interaction with folate status. Proc Natl Acad Sci U S A 2002;99:5606-11.
2. Domagala TB, Undas A, Libura M, Szczeklik A. Pathogenesis of vascular disease in hyperhomocysteinaemia [Review]. J Cardiovasc Risk 1998;5:239-47.
3. Dong C, Yoon W, Goldschmidt-Clermont PJ. DNA methylation and atherosclerosis [Review]. J Nutr 2002;132:2406S-9S.
4. Bestor TH. The DNA methyltransferases of mammals [Review]. Hum Mol Genet 2000;9:2395-402.
5. Vilkaitis G, Merkiene E, Serva S, Weinhold E, Klimasauskas S. The mechanism of DNA cytosine-5 methylation. Kinetic and mutational dissection of Hhal methyltransferase. J Biol Chem 2001;276:20924-34.
6. Clark SJ, Harrison J, Frommer M. CpNpG methylation in mammalian cells. Nat Genet 1995;10:20-7.
7. Okano M, Bell DW, Haber DA, Li E. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 1999;99:247-57.
8. Cantoni GL. The role of S-adenosylhomocysteine in the biological utilization of S-adenosylmethionine. Prog Clin Biol Res 1985;198:47-65.
9. Chiang PK, Cantoni GL. Perturbation of biochemical transmethylations by 3-deazaadenosine in vivo. Biochem Pharmacol 1979;28:1897-902.
10. Hoffman DR, Marion DW, Cornatzer WE, Duerre JA. S-Adenosylme-thionine and S-adenosylhomocystein metabolism in isolated rat liver. Effects of L-methionine, L-homocystein, and adenosine. J Biol Chem 1980;255:10822-7.
11. Hoffman DR, Cornatzer WE, Duerre JA. Relationship between tissue levels of S-adenosylmethionine, S-adenylhomocysteine, and transmethylation reactions. Can J Biochem 1979;57:56-65.
12. Melnyk S, Pogribna M, Pogribny IP, Yi P, James SJ. Measurement of plasma and intracellular S-adenosylmethionine and S-adenosylhomocysteine utilizing coulometric electrochemical detection: alterations with plasma homocysteine and pyridoxal 5′-phosphate concentrations. Clin Chem 2000;46:265-72.
13. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988;16:1215.
14. Leadon SA, Cerutti PA. A rapid and mild procedure for the isolation of DNA from mammalian cells. Anal Biochem 1982;120:282-8.
15. Struys EA, Jansen EE, de Meer K, Jakobs C. Determination of S-adenosylmethionine and S-adenosylhomocysteine in plasma and cerebrospinal fluid by stable-isotope dilution tandem mass spectrometry. Clin Chem 2000;46:1650-6.
16. Pogribny I, Yi P, James SJ. A sensitive new method for rapid detection of abnormal methylation patterns in global DNA and within CpG islands. Biochem Biophys Res Commun 1999;262:624-8.
17. James SJ, Melnyk S, Pogribna M, Pogribny IP, Caudill MA. Elevation in S-adenosylhomocysteine and DNA hypomethylation: potential epigenetic mechanism for homocysteine-related pathology. J Nutr 2002;132:2361S-6S.
18. Caudill MA, Wang JC, Melnyk S, Pogribny IP, Jernigan S, Collins MD, et al. Intracellular S-adenosylhomocysteine concentrations predict global DNA hypomethylation in tissues of methyl-deficient cystathionine β-synthase heterozygous mice. J Nutr 2001;131:2811-8.
19. van der Molen EF, Hiipakka MJ, van Lith-Zanders H, Boers GH, van den Heuvel LP, Monnens LA, et al. Homocysteine metabolism in endothelial cells of a patient homozygous for cystathionine β-synthase (CS) deficiency. Thromb Haemost 1997;78:827-33.
20. Yi P, Melnyk S, Pogribna M, Pogribny IP, Hine RJ, James SJ. Increase in plasma homocysteine associated with parallel increases in plasma S-adenosylhomocysteine and lymphocyte DNA hypomethylation. J Biol Chem 2000;275:29318-23.