Quantification of Serum and Urinary S-Adenosylmethionine and S-Adenosylhomocysteine by Stable-Isotope-Dilution Liquid Chromatography-Mass Spectrometry

Clinical Chemistry

Volumn 50, Issue 2, 2004, Pages 365-372

  Stabler, Sally P ^a,b   Allen, Robert H ^a  

^a UNIVERSITY OF COLORADO   (United States)

^b Box B170 ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON 13; S ADENOSYLHOMOCYSTEINE; S ADENOSYLMETHIONINE;

ACCURACY; ARTICLE; CONTROLLED STUDY; DILUTION; ERYTHROCYTE; HUMAN; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NORMAL HUMAN; QUANTITATIVE ANALYSIS; ROOM TEMPERATURE; STANDARDIZATION; URINALYSIS;

ADULT; ANIMALS; CHROMATOGRAPHY, LIQUID; ERYTHROCYTES; FEMALE; HUMANS; INDICATOR DILUTION TECHNIQUES; LIVER; LUNG; MALE; MASS SPECTROMETRY; MIDDLE AGED; RATS; REFERENCE VALUES; S-ADENOSYLHOMOCYSTEINE; S-ADENOSYLMETHIONINE;

EID: 0742269630     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2003.026252     Document Type: Article

References (27)

1. Stabler SP, Marcell PD, Podell ER, Savage DG, Lindenbaum J, Allen RH. Quantitation of total homocysteine, total cysteine, and methionine in normal serum using capillary gas chromatography-mass spectrometry. Anal Biochem 1987;162:185-96.
2. Savage DG, Lindenbaum J, Stabler SP, Allen RH. Sensitivity of serum methylmalonic acid and total homocysteine determinations for diagnosing cobalamin and folate deficiency. Am J Med 1994; 96:239-46.
3. Soria C, Chadefaux B, Coude M Gaillard O, Kamoun P. Concentrations of total homocysteine in plasma in chronic renal failure [Technical Brief]. Clin Chem 1990;36:2137-8.
4. Bostom AG, Lathrop L. Hyperhomocysteinemia in end stage renal disease: prevalence, etiology, and potential relationship to arteriosclerotic outcomes. Kidney Int 1997;52:10-20.
5. Ueland PM, Refsum H. Review article: plasma homocysteine, a risk factor for vascular disease, plasma levels in health, disease, and drug therapy. J Lab Clin Med 1989;114:473-501.
6. Vollset SE, Refsum H, Tverdal A, Nygard O, Nordrehaug JE, Tell GS, et al. Plasma total homocysteine and cardiovascular and noncardiovascular mortality: the Hordaland Homocysteine Study. Am J Clin Nutr 2001;74:130-6.
7. Weir DG, Scott JM. The biochemical basis of the neuropathy in cobalamin deficiency. Baillieres Clin Haematol 1995;8:479-97.
8. Mudd SH, Levy HL, Svovby F. Disorders of transsulfurations. In: Scriver CR, Beaudet AL, Sly WS, Valle D, eds. The metabolic and molecular bases of inherited disease, 7th ed. New York: McGraw-Hill, 1995:1279-327.
9. Finkelstein JD, Kyle WE, Harris BJ. Methionine metabolism in mammals: regulatory effects of S-adenosylhomocysteine. Arch Biochem Biophys 1974;165:774-9.
10. De la Haba G, Cantoni GL. The enzymatic synthesis of S-adenosyl-L- homocysteine from adenosine and homocysteine. J Biol Chem 1959;234:603-8.
11. Molloy AM, Orsi B, Kennedy DG, Kennedy S, Weir DG, Scott JM. The relationship between the activity of methionine synthase and the ratio of S-adenosylmethionine to S-adenosylhomocysteine in the brain and other tissues of the pig. Biochem Pharmacol 1992;44:1349-55.
12. Perna AF, Ingrosso D, Zappia V, Galletti P, Capasso G, De Santo NG. Enzymatic methyl esterification of erythrocyte membrane proteins is impaired in chronic renal failure. Evidence for high levels of the natural inhibitor S-adenosylhomocysteine. J Clin Invest 1993;91:2497-503.
13. Fu W, Dudman NP, Perry MA, Young K, Wang XL. Interrelations between plasma homocysteine and intracellular S-adenosylhomocysteine. Biochem Biophys Res Commun 2000;271:47-53.
14. Loehrer FM, Haefeli WE, Angst CP, Browne G, Frick G, Fowler B. Effect of methionine loading on 5-adenosylmethionine and S-adenosylhomocysteine in plasma of healthy humans. Clin Sci 1996;91:79-86.
15. Capdevila A, Wagner C. Measurement of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent isoindoles. Anal Biochem 1998;264:180-4.
16. Melnyk S, Pogribna M, Pogribny IP, Yi P, James SJ. Measurement of plasma and intracellular S-adenosylmethionine and S-adenosylhomocysteine utilizing coulometric electrochemical detection: alterations with plasma homocysteine and pyridoxal 5′-phosphate concentrations [Erratum published in Clin Chem 2001;47:612]. Clin Chem 2000;46:265-72.
17. Struys EA, Jansen EE, de Meer K, Jakobs C. Determination of S-adenosylmethionine and S-adenosylhomocysteine in plasma and cerebrospinal fluid by stable isotope dilution tandem mass spectrometry. Clin Chem 2000;46:1650-6.
18. Yi P, Melnyk S, Pogribna M, Pogribny IP, Hine RJ, James SJ. Increase in plasma homocysteine associated with parallel increases in plasma S-adenosylhomocysteine and lymphocyte DNA hypomethylation. J Biol Chem 2000;275:29318-23.
19. Kerins DM, Koury MJ, Capdevila A, Rana S, Wagner C. Plasma S-adenosylhomocysteine is a more sensitive indicator of cardiovascular disease than plasma homocysteine. Am J Clin Nutr 2001;74:723-9.
20. Guerra-Shinohara EM, Morita OE, Pagliusi RA, Blaia-D'Avila VL, Allen RH, Stabler SP. Elevation of serum S-adenosylhomocysteine in severe cobalamin deficient megaloblastic anemia [Abstract]. Blood 2001;98:13a.
21. Stabler SP, Lindenbaum J, Savage DG, Allen RH. Elevation of serum cystathionine levels in patients with cobalamin and folate deficiency. Blood 1993;81:3404-13.
22. Allen, RH, Stabler SP, Savage DG, Lindenbaum J. Elevation of 2-methycitric acid I and II levels in serum, urine, and cerebrospinal fluid of patients with cobalamin deficiency. Metabolism 1993;42: 978-88.
23. Delabar U, Kloor D, Luippold G, Muhlbauer B. Simultaneous determination of adenosine, S-adenosylhomocysteine and S-adenosylmethionine in biological samples using solid-phase extraction and high performance liquid chromatography. J Chromatogr B Biomed Sci Appl 1999;724:231-8.
24. Loehrer FMT, Angst CP, Brunner FP, Haefeli WE, Fowler B. Evidence for disturbed S-adenosylmethionine: S-adenosylhomocysteine ratio in patients with end stage renal failure: a cause for disturbed methylation reactions? Nephrol Dial Transplant 1998; 13:656-61.
25. Castro R, Rivera I, Struys EA, Jansen EEW, Ravasco P, Camilo ME, et al. Increased homocysteine and S-adenosylhomocysteine concentrations and DNA hypomethylation in vascular disease. Clin Chem 2003;49:1292-6.
26. 6 concentrations. Eur J Clin Invest 2003;33:17-25.
27. Stabler SP, Allen RH, Dolce ET, Hawthorne NA, Johnson MA. Response of elevated serum S-adenosylhomocysteine and total homocysteine to high dose oral cobalamin [Abstract]. Blood 2002;100:227a.