Cloning and characterization of the pyrH gene encoding UMP-kinase from Lactobacillus reuteri ATCC 55739

Journal of Microbiology and Biotechnology

Volumn 15, Issue 3, 2005, Pages 525-531

  Park, Jae Yong ^a   Nam, Su Jin ^a   Kim, Jong Hwan ^a   Jeong, Seon Ju ^a   Kim, Jung Kon ^a   Ha, Yeong Lae ^a   Kim, Jeong Hwan ^a  

^a Gyeongsang National University   (South Korea)

Author keywords

Lactobacillus reuteri; Operon; pyrH; Ribosomal recycling factor; UMP kinase

Indexed keywords

ELONGATION FACTOR TS; PHOSPHOTRANSFERASE; URIDINE PHOSPHATE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; CONTROLLED STUDY; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE IDENTIFICATION; GENE LIBRARY; GENE MAPPING; GENE OVEREXPRESSION; GENE SEQUENCE; GENETIC CODE; GENETIC SCREENING; LACTOBACILLUS REUTERI; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SOUTHERN BLOTTING;

LACTOBACILLUS REUTERI;

EID: 22544486570     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Blank, J., S. Nock, R. Kreutzer, and M. Sprinzl. 1996. Elongation factor Ts from Thermus thermophilus overproduction in Escherichia coli, quaternary structure and interaction with elongation factor Tu. Eur. J. Biochem. 236: 222-227.
2. Bucurenci, N., L. Serina, C. Zaharia, S. Landais, A. Danchin, and O. Bârzu. 1998. Mutational analysis of UMP kinase from Escherichia coli. J. Bacteriol. 180: 473-477.
3. Dower, W. J., J. F. Miller, and C. W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16: 6127-6145.
4. Ingraham, J. L. and J. Neuhard. 1972. Cold-sensitive mutants of Salmonella typhimurium defective in uridine monophosphate kinase. J. Biol. Chem. 247: 6259-6265.
5. Jeong, S. J., D. J. You, H. J. Kwon, S. Kanaya, N. Kunihiro, K. H. Kim, Y. H. Kim, and B. W. Kim. 2002. Cloning and characterization of cycloinulooligosaccharide fructanotransferase (CFTase) from Bacillus polymyxa MGL21. J. Microbiol. Biotechnol. 12: 921-928.
6. Kim, T. Y., J. M. Lee, H. C. Chang, D. K. Chung, J. H. Lee, J. H. Kim, and H. J. Lee. 1999. Effect of temperature and a carbon source on the expression of β-galactosidase gene of Lactococcus lactis ssp. lactis ATCC 7962. J. Microbiol. Biotechnol. 9: 201-205.
7. Laemmli, U. K. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
8. Lee, K. H., G. S. Moon, J. Y. An, H. J. Lee, H. C. Cahng, D. K. Chung, J. H. Lee, and J. H. Kim. 2002. Isolation of a nisin-producing Lactococcus lactis strain from kimchi and characteriaztion of its nisZ gene. J. Microbiol. Biotechnol. 12: 389-397.
9. Ohnishi, M., L. Janosi, M. Shuda, H. Matsumoto, T. Hayashi, Y. Terawaki, and A. Kaji. 1999. Molecular cloning, sequencing, purification, and characterization of Pseudomonas aeruginosa ribosome recycling factor. J. Bacteriol. 181: 1281-1291.
10. Park, J. Y., S. J. Park, S. J. Nam, Y. L. Ha, and J. H. Kim. 2002. Cloning and characterization of L-lactate dehydrogenase gene (ldhL) from Lactobacillus reuteri ATCC 55739. J. Microbiol. Biotechnol. 12: 716-721.
11. Park, R.-J., K.-H. Lee, S.-J. Kim, J.-Y. Park, S.-J. Nam, H.-D. Yun, H.-J. Lee, H. C. Chang, D. K. Chung, J.-H. Lee, Y H. Park, and J. H. Kim. 2002. Isolation of Lactococcus lactis strain with β-galactosidase activity from kimchi and cloning of lacZ gene from the isolated strain. J. Microbiol. Biotechnol. 12: 157-161.
12. Saavedra, J. 2000. Probiotics and infectious diarrhea. Am. J. Gastroenterol. 95: S16-S18.
13. Sambrook, J. E. F. Fritsch, and J. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., U.S.A.
14. Serina, L., C. Blondin, E. Krin, O. Sismeiro, A. Danchin, H. Sakamoto, A.-M. Gilles, and O. Barzu. 1995. Escherichia coli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine nucleotides and UTP. Biochemistry 34: 5066-5074.
15. Smallshaw, J. and R. A. Kelln. 1992. Cloning, nucleotide sequence and expression of the Escherichia coli K-12 pyrH gene encoding UMP kinase. Genetics (Life Sci. Adv.) 11: 59-65.
16. Talarico, T. L., I. A. Casas, T. C. Chung, and W. J. Dobrogosz. 1988. Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri. Antimicrob. Agents Chemother. 32: 1854-1858.
17. Wadskov-Hansen, S. L., J. Martinussen, and K. Hammer. 2000. The pyrH gene of Lactococcus lactis subsp. cremoris encoding UMP kinase is transcribed as part of an operon including the frr1 gene encoding ribosomal recycling factor 1. Gene 241: 157-166.