Elongation factor ts from thermus thermophilus - Overproduction in Escherichia coli, quaternary structure and interaction with elongation factor Tu

European Journal of Biochemistry

Volumn 236, Issue 1, 1996, Pages 222-227

  Blank, J ^a   Nock, S ^a   Kreutzer, R ^a   Sprinzl, M ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

Disulfide bridge; EF Ts; EF Tu; Nucleotide exchange; Protein biosynthesis

Indexed keywords

DISULFIDE; ELONGATION FACTOR TS; ELONGATION FACTOR TU;

AMINO ACID SEQUENCE; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN QUATERNARY STRUCTURE; THERMUS THERMOPHILUS;

EID: 0029670027     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.00222.x     Document Type: Article

References (33)

1. An, G., Bendiak, D. S., Mamelak, L. A. & Friesen, J. D. (1981) Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts, Nucleic Acids Res. 9, 4163–4172.
2. Arai, K.‐I., Arai, N., Nakamura, S., Oshima, T. & Kaziro, Y. (1978a) Studies on polypeptide‐chain‐elongation factors from an extreme thermophile, Thermus thermophilus HB8, Eur. J. Biochem. 92, 521–531.
3. Arai, K.‐I., Kawakita, M. & Kaziro, Y. (1972) Studies on polypeptide elongation factors from Escherichia coli, J. Biol. Chem. 247, 7029–7037.
4. Arai, K.‐I., Ota, Y., Arai, N., Nakamura, S., Hennecke, C., Oshima, T. & Kaziro, Y. (1978b) Studies on polypeptide‐chain‐elongation factors from an extreme thermophile, Thermus thermophilus HB8, Eur. J. Biochem. 92, 509–519.
5. Arcari, P., Raimo, G., Ianniciello, G., Gallo, M. & Bocchini, V. (1995) The first nucleotide sequence of an archaeal elongation factor 1β gene, Biochim. Biophys. Acta 1263, 86–88.
6. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Smith, J. A., Seidman, J. G. & Struhl, K. (1987) Current Protocols Mol. Biol, Greene Publishing Associates, New York.
7. Berchtold, H., Reshetnikova, L., Reiser, C. O. A., Schirmer, N. K., Sprinzl, M. & Hilgenfeld, R. (1993) Crystal structure of active elongation factor Tu reveals major domain rearrangements, Nature 365, 126–132.
8. Blank, J., Grillenbeck, N. W., Kreutzer, R. & Sprinzl, M. (1995) Overexpression and purification of Thermus thermophilus elongation factors G, Tu and Ts from E. coli, Protein Express. & Purif. 6, 637–645.
9. Bøgestrand, S., Wiborg, O., Thirup, S. & Nyborg, J. (1995) Analysis and crystallization of a 25 kDa C‐terminal fragment of cloned elongation factor Ts from Escherichia coli, FEBS Lett. 368, 49–54.
10. Bonfini, L., Karlovich, C. A., Dasgupta, C. & Banerjee, U. (1992) The Son of sevenless gene product: a putative activator of Ras, Science 255, 603–606.
11. Bubunenko, M. G., Kireeva, M. L. & Gudkov, A. T. (1992) Novel data on interactions of elongation factor Ts, Biochimie (Paris) 74, 419–425.
12. Chevalier, C., Saillard, C. & Bové, J. M. (1990) Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein, J. Bacteriol. 172, 2693–2703.
13. Eccleston, J. F. (1984) A kinetic analysis of the interaction of elongation factor Tu with guanosine nucleotides and elongation factor Ts, J. Biol. Chem. 259, 12997–13003.
14. Eccleston, J. F., Gratton, E. & Jameson, D. M. (1987) Interaction of a fluorescent analogue of GDP with elongation factor Tu: steady‐state and time‐resolved fluorescence studies, Biochem. 26, 3902–3907.
15. Ehrenberg, M., Rojas, A. M., Weiser, J. & Kurland, C. G. (1990) How many EF‐Tu molecules participate in aminoacyl‐tRNA binding and peptide bond formation in Escherichia coli translation, J. Mol. Biol. 211, 739–749.
16. Fleischmann, R. D., Adams, M. D., White, O., Clayton, R. A., Kirkness, E. F., Kerlavage, A. R., Bult, C. J., Tomb, J.‐F., Dougherty, B. A., Merrick, J. M., McKenney, K., Sutton, G., FitzHugh, W., Fields, C., Gocayne, J. D. (1995) Whole‐genome random sequencing and assembly of Haemophilus influenzae Rd, Science 269, 496–512.
17. Kjeldgaard, M. & Nyborg, J. (1992) Refined structure of elongation factor EF‐Tu from Escherichia coli, J. Mol. Biol. 223, 721–742.
18. Kjeldgaard, M., Nissen, P., Thirup, S. & Nyborg, J. (1993) The crystal structure of elongation factor EF‐Tu from Thermus aquaticus in the GTP conformation, Structure 1, 35–50.
19. Kostrzewa, M. & Zetsche, K. (1993) Organization of plastid‐encoded ATPase genes and flanking regions including homologues of infB and tsf in the thermophilic red alga Galdieria sulphuraria, Plant Mol. Biol. 23, 67–76.
20. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680–685.
21. Miller, D. L. & Weissbach, H. (1977) in Molecular mechanisms of protein biosynthesis ( Weissbach, H. & Prestka, S., eds) pp. 323–373, Academic Press, New York.
22. Robinson, L. C., Gibbs, J. B., Marshall, M. S., Sigal, I. S. & Tatchell, K. (1987) CDC25: a component of the RAS‐adenylate cyclase pathway in Saccharomyces cerevisiae, Science 235, 1218–1221.
23. Rodnina, M. V. & Wintermeyer, W. (1995) GTP consumption of elongation factor Tu during translation of heteropolymeric mRNAs, Proc. Natl Acad. Sci. USA 92, 1945–1949.
24. Romero, G., Chau, V. & Biltonen, R. L. (1985) Kinetics and thermodynamics of the interaction of elongtion factor Tu with elongation factor Ts, guanine nucleotides, and aminoacyl‐tRNA, J. Biol. Chem. 260, 6167–6174.
25. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Press, New York.
26. Sanangelantoni, A. M., Calogero, R. C., Buttarelli, F. R., Gualerzi, C. O. & Tiboni, O. (1990) Organization and nucleotide sequence of the genes for ribosomal protein S2 and elongation factor Ts in Spirulina platensis, FEMS Microbiol. Lett. 66, 141–146.
27. Schägger, H. & von Jagow, G. (1987) Tricinesodium dodecyl sulfatepolyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Anal. Biochem. 166, 368–379.
28. Schirmer, N. K., Reiser, C. O. A. & Sprinzl, M. (1991) Effect of Thermus thermophilus elongation factor Ts on the conformation of elongation factor Tu, Eur. J. Biochem. 200, 295–300.
29. Weijland, A. & Parmeggiani, A. (1993) Toward a model for the interaction between elongation factor Tu and the ribosome, Science 259, 1311–1314.
30. Whittaker, P. A., Campbell, A. J. B., Southern, E. M. & Murray, N. E. (1988) Enhanced recovery and restriction mapping of DNA fragments cloned in a new λ vector, Nucleic Acids Res. 16, 6725–6736.
31. Xin, H., Woriax, V., Burkhart, W. & Spremulli, L. L. (1995) Cloning and expression of mitochondrial translational elongation factor Ts from bovine and human liver, J. Biol. Chem. 270, 17243–17249.
32. Yanisch‐Perron, C., Vieira, J. & Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13‐mp18 and pUC19 vectors, Gene (Amst.) 33, 103–119.
33. Young, R. A. & Davis, R. W. (1983) Yeast RNA polymerase II genes: isolation with antibody probes, Science 222, 778–782.