Development of a PCR-based method for the detection of enteric myxozoans causing the emaciation disease of cultured tiger puffer

Fish Pathology

Volumn 40, Issue 1, 2005, Pages 22-28

  Yanagida, Tetsuya ^a   Freeman, Mark A ^a   Nomura, Yoshinori ^b   Takami, Ikuo ^c   Sugihara, Yukitaka ^c   Yokoyama, Hiroshi ^a   Ogawa, Kazuo ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b Kumamoto Prefectural Fisheries Research Center   (Japan)

^c NAGASAKI PREFECTURAL INSTITUTE OF FISHERIES   (Japan)

Author keywords

Diagnosis; Enteromyxum; Leptotheca; Myxosporean emaciation disease; PCR; SSU rDNA; Takifugu rubripes

Indexed keywords

ENTEROMYXUM; ENTEROMYXUM LEEI; LEPTOTHECA; LEPTOTHECA FUGU; MYXOSPOREA; MYXOZOA; TAKIFUGU; TAKIFUGU RUBRIPES; TETRAODONTIDAE;

EID: 22544440276     PISSN: 0388788X     EISSN: None     Source Type: Journal    
DOI: 10.3147/jsfp.40.23     Document Type: Article

References (19)

1. Diamant, A. (1997): Fish-to-fish transmission of a marine myxosporean. Dis. Aquat. Org., 30, 99-105.
2. Fox, M. D., O. Palenzuela and J. L. Bartholomew (2000): Strategies for diagnosis of Ceratomyxa shasta using PCR: comparison of lethal and non-lethal sampling with microscopic examination. J. Aquat. Anim. Health, 12, 100-106.
3. Gonzalez, S. F., M. J. Krug, M. E. Nielsen, Y. Santos and D. R. Call (2004): Simultaneous detection of marine fish pathogens by using multiplex PCR and a DNA microarray. J. Clin. Microbiol., 42, 1414-1419.
4. Køie, M., C. M. Whipps and M. L. Kent (2004): Ellipsomyxa gobii (Myxozoa: Ceratomyxidae) in the common goby Pomatoschistus microps (Teleostei: Gobiidae) uses Nereis spp. (Annelida: Polychaeta) as invertebrate hosts. Folia Parasitol., 51, 14-18.
5. Morris, D. J., A. Adams, S. W. Feist, J. MacGeorge and R. H. Richards (2000): Immunohistochemical and PCR studies of wild fish for Tetracapsula bryosalmonae (PKX), the causative organism of proliferative kidney disease. J. Fish Dis., 23, 129-135.
6. Morris, D. C., D. J. Morris and A. Adams (2002): Development of improved PCR to prevent false negative in the detection of Tetracapsula bryosalmonae, the causative agent of proliferative kidney disease. J. Fish Dis., 25, 483-490.
7. Ogawa, K. and H. Yokoyama (2001): Emaciation disease of cultured tiger puffer Takifugu rubripes. Bull. Natl. Res. Inst. Aquacult., Suppl., 5, 65-70.
8. Russell, S., S. Fraska Jr., I. Sunila and R. A. French (2004): Application of a multiplex PCR for the detection of protozoan pathogens of the eastern oyster Crassostrea virginica in field samples. Dis. Aquat. Org., 59, 85-91.
9. Saulnier, D. and P. de Kinkelin (1997): Polymerase chain reaction primers for investigations on the causative agent of proliferative kidney disease of salmonids. J. Fish Dis., 20, 467-470.
10. Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin and D. G. Higgins (1997): The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res., 25, 4876-4882.
11. Tin Tun (2000): Myxosporean and hyperparasitic microsporean infections in the intestine of cultured tiger puffer. PhD thesis, The University of Tokyo, 90 p.
12. Tin Tun, K. Ogawa and H. Wakabayashi (2002): Pathological changes induced by three myxosporeans in the intestine of cultured tiger puffer, Takifugu rubripes (Temminck and Schlegel). J. Fish Dis., 25, 63-72.
13. Tin Tun, H. Yokoyama, K. Ogawa and H. Wakabayashi (2000): Myxosporeans and their hyperparasitic microsporeans in the intestine of emaciated tiger puffer. Fish Pathol., 35, 145-156.
14. Wakabayashi, H. (1996): Importation of aquaculture seedlings to Japan. Rev. Sci. Tech. OIE, 15, 409-422.
15. Whitaker J. W., L. M. Pote, L. Khoo, R. Shivaji and L. A. Hanson (2001): The use of polymerase chain reaction assay to diagnose proliferative gill disease in channel catfish (Ictalurus punctatus). J. Vet. Diagn. Invest., 13, 394-398.
16. Yanagida, T., Y. Nomura, T. Kimura, Y. Fukuda, H. Yokoyama and K. Ogawa (2004): Molecular and morphological redescriptions of enteric myxozoans, Enteromyxum leei (formerly Myxidium sp. TP) and Enteromyxum fugu comb. n. (syn. Myxidium fugu) from cultured tiger puffer. Fish Pathol., 39, 137-143.
17. Yasuda, H., T. Ooyama, K. Iwata, Tin Tun, H. Yokoyama and K. Ogawa (2002): Fish-to-fish transmission of Myxidium spp. (Myxozoa) in cultured tiger puffer suffering from emaciation disease. Fish Pathol., 37, 29-33.
18. Yokoyama, H. (2003): A review: gaps in our knowledge of myxozoans of fishes. Fish Pathol., 38, 125-136.
19. Yokoyama, H., D. Inoue, A. Sugiyama and H. Wakabayashi (2000): Polymerase chain reaction and indirect fluorescent antibody technique for the detection of Kudoa amamiensis (Multivalvulida: Myxozoa) in yellowtail Seriola quinqueradiata. Fish Pathol., 35, 157-162.