Purification and characterization of a new endo-1,4-β-D-glucanase from Beltraniella portoricensis

Bioscience, Biotechnology and Biochemistry

Volumn 69, Issue 6, 2005, Pages 1198-1201

  Baba, Yuko ^a   Shimonaka, Atsushi ^a   Koga, Jinichiro ^a   Murashima, Koichiro ^a   Kubota, Hidetoshi ^a   Kono, Toshiaki ^a  

^a MEIJI SEIKA KAISHA LTD   (Japan)

Author keywords

Beltraniella portoricensis; Cellulase; Detergent; Endoglucanase; Family 45 glycoside hydrolase

Indexed keywords

AMINO ACIDS; CELL CULTURE; CHARACTERIZATION; ELECTROPHORESIS; MOLECULAR WEIGHT; NEGATIVE IONS; OXIDATION; PH EFFECTS; PURIFICATION; SURFACE ACTIVE AGENTS; THERMAL EFFECTS;

CARBOXYMETHYL CELLULASE (CMCASE); ENDOGLUCANASE; RHIZOPUS ORYZAE; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE);

ENZYMES;

CELLULASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; DEUTEROMYCETES; ENZYME STABILITY; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PH; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; TEMPERATURE;

AMINO ACID SEQUENCE; CELLULASE; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; MITOSPORIC FUNGI; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE;

BELTRANIELLA; HUMICOLA GRISEA; PHYCOMYCES; PHYCOMYCETES; RHIZOPUS ORYZAE;

EID: 22444432914     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.69.1198     Document Type: Article

References (18)

1. Henrissat, B., Driguez, H., Viet, C., and Schülein, M., Synergism of cellulases from Trichoderma reesei in the degradation of cellulose. Bio/Technology, 3, 722-726 (1985).
2. Cavaco-Paulo, A., Mechanism of cellulase action in textile processes. Carbohydr. Polym., 37, 273-277 (1998).
3. Azevedo, H., Bishop, D., and Cavaco-Paulo, A., Effects of agitation level on the adsorption, desorption, and activities on cotton fabrics of full length and core domains of EGV (Humicola insolens) and CenA (Cellulomonas fimi). Enzyme Microb. Technol., 27, 325-329 (2000).
4. Bhat, M. K., and Bhat, S., Cellulose degrading enzymes and their potential industrial applications. Biotechnol. Adv., 15, 583-620 (1997).
5. Bhat, M. K., Cellulases and related enzymes in biotechnology. Biotechnol. Adv., 18, 355-383 (2000).
6. Hirvonen, M., and Papageorgiou, A. C., Crystal structure of a family 45 endoglucanase from Melanocarpus albomyces: mechanistic implications based on the free and cellobiose-bound forms. J. Mol. Biol., 329, 403-410 (2003).
7. Nakamura, Y., Moriya, T., Baba, Y., Yanai, K., Sumida, N., Nishimura, T., Murashima, K., Nakane, A., Yaguchi, T., Koga, J., Murakami, T., and Kono, T., WO patent, 00/24879 (May 4, 2000).
8. Otzen, D. E., Christiansen, L., and Schülein, M., A comparative study of the unfolding of the endoglucanase Cel45 from Humicola insolens in denaturant and surfactant. Protein Sci., 8, 1878-1887 (1999).
9. Miller, G. L., Use of dinitrosalicyclic acid reagent for determination of reducing sugar. Anal. Chem., 31, 426-428 (1959).
10. Coutinho, P. M., and Henrissat, B., Carbohydrate-active enzymes server at URL: http://afmb.cnrs-mrs.fr/CAZY/GH_45.html (1999).
11. Murashima, K., Nishimura, T., Nakamura, Y., Koga, J., Moriya, T., Sumida, N., Yaguchi, T., and Kono, T., Purification and characterization of new endo-1,4-β-D-glucanases from Rhizopus oryzae. Enzyme Microb. Technol., 30, 319-326 (2002).
12. Moriya, T., Murashima, K., Nakane, A., Yanai, K., Sumida, N., Koga, J., Murakami, T., and Kono, T., Molecular cloning of endo-β-D-1,4-glucanase genes, rce1, rce2, and rce3, from Rhizopus oryzae. J. Bacteriol., 185, 1749-1756 (2003).
13. Shimonaka, A., Baba, Y., Koga, J., Nakane, A., Kubota, H., and Kono, T., Molecular cloning of a gene encoding endo-β-D-1,4-glucanase PCE1 from Phycomyces nitens. Biosci. Biotechnol. Biochem., 68, 2299-2305 (2004).
14. Takashima, S., Iikura, H., Nakamura, A., Hidaka, M., Masaki, H., and Uozumi, T., Comparison of gene structures and enzymatic properties between two endoglucanases from Humicola grisea. J. Biotechnol., 67, 85-97 (1999).
15. Murashima, K., Moriya, T., Hamaya, T., Koga, J., Sumida, N., Aoyagi, K., Murakami, T., and Kono, T., WO patent, 98/03640 (Jan. 29, 1998).
16. Murashima, K., Sumida, N., Nakane, A., Yanai, K., Nishimura, T., Koga, J., Murakami, T., and Kono, T., WO patent, 01/90375 (Nov. 29, 2001).
17. Davies, G. J., Dodson, G. G., Hubbard, R. E., Tolley, S. P., Dauter, Z., Wilson, K. S., Hjort, C., Mikkelsen, J. M., Rasmussen, G., and Schülein, M., Structure and function of endoglucanase V. Nature, 365, 362-364 (1993).
18. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).