Use of Clostridium septicum alpha toxins for isolation of various glycosylphosphatidylinositol-deficient cells

Journal of Microbiology

Volumn 43, Issue 3, 2005, Pages 266-271

  Shin, Dong Jun ^a   Choy, Hyon E ^a   Hong, Yeongjin ^a  

^a Chonnam National University Medical School   (South Korea)

Author keywords

Aerolysin; Aeromonas hydrophila; Alpha toxins; Clostridium septicum; Glycosylphosphatidylinositol

Indexed keywords

ACYLTRANSFERASE; AEROLYSIN; BACTERIAL TOXIN; GLYCOSYLPHOSPHATIDYLINOSITOL; HEMOLYTIC TOXIN, CLOSTRIDIUM SEPTICUM; INOSITOL; MANNOSYLTRANSFERASE; PORE FORMING CYTOTOXIC PROTEIN;

ANIMAL; ARTICLE; CHO CELL; CRICETULUS; DRUG EFFECT; FLOW CYTOMETRY; GENETIC TRANSFECTION; GENETICS; HAMSTER; METABOLISM; MUTATION; PLASMID;

ACYLTRANSFERASES; ANIMALS; BACTERIAL TOXINS; CHO CELLS; CRICETINAE; CRICETULUS; FLOW CYTOMETRY; GLYCOSYLPHOSPHATIDYLINOSITOLS; INOSITOL; MANNOSYLTRANSFERASES; MUTATION; PLASMIDS; PORE FORMING CYTOTOXIC PROTEINS; TRANSFECTION;

AEROMONAS HYDROPHILA; CLOSTRIDIUM SEPTICUM; CRICETULUS GRISEUS; EUKARYOTA; NEGIBACTERIA; POSIBACTERIA; SUS SCROFA;

EID: 22244484054     PISSN: 12258873     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Abrami, L., M. Fivaz, and F.G. van der Goot. 2000. Surface dynamics of aerolysin on the plasma membrane of living cells. Int. J. Med. Microbiol. 290, 363-367.
2. Ballard, J., A. Bryant, D. Stevens, and R.K. Tweten. 1992. Purification and characterization of the lethal toxin (alpha-toxin) of Clostridium septicum. Infect. Immun. 60, 784-790.
3. Ballard, J., J. Crabtree, B.A. Roe, and R.K. Tweten. 1995. The primary structure of Clostridium septicum alpha-toxin exhibits similarity with that of Aeromonas hydrophila aerolysin. Infect. Immun. 63, 340-344.
4. Diep, D.B., K.L. Nelson, S.M. Raja, E.N. Pleshak, and J.T. Buckley. 1998. Glycosylphosphatidylinositol anchors of membrane glycoproteins are binding determinants for the channel-forming toxin aerolysin. J. Biol. Chem. 273, 2355-2360.
5. Eisenhaber, B., S. Maurer-Stroh, M. Novatchkova, G. Schneider, and F. Eisenhaber. 2003. Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins. Bioessays 25, 367-385.
6. Ferguson, M.A. 1999. The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research. J. Cell. Sci. 112 (Pt 17), 2799-2809.
7. Fivaz, M., L. Abrami, Y. Tsitrin, and F.G. van der Goot. 2001. Aerolysin from Aeromonas hydrophila and related toxins. Curr. Top. Microbiol. Immunol. 257, 35-52.
8. Gordon, V.M., K.L. Nelson, J.T. Buckley, V.L. Stevens, R.K. Tweten, P.C. Elwood, and S.H. Leppla. 1999. Clostridium septicum alpha toxins uses glycosylphosphatidylinositol-anchored protein receptors. J. Biol. Chem. 274, 27274-27280.
9. Gowda, D.C. and E.A. Davidson. 1999. Protein glycosylation in the malaria parasite. Parasitol. Today 15, 147-152.
10. Hong, Y., K. Ohishi, J.Y. Kang, S. Tanaka, N. Inoue, J. Nishimura, Y. Maeda, and T. Kinoshita. 2003. Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins. Mol. Biol. Cell. 14, 1780-1789.
11. Hong, Y., K. Ohishi, N. Inoue, J.Y. Kang, H. Shime, Y. Horiguchi, F.G. van der Goot, N. Sugimoto, and T. Kinoshita. 2002. Requirement of N-glycan on GPI-anchored proteins for efficient binding of aerolysin but not Clostridium septicum alpha-toxin. EMBO J. 21, 5047-5056.
12. Ikezawa, H. 2002. Glycosylphosphatidylinositol (GPI)-anchored proteins. Biol. Pharm. Bull. 25, 409-417.
13. Kang, J.Y., Y. Hong, H. Ashida, N. Shishioh, Y. Murakami, Y.S. Morita, Y. Maeda, and T. Kinoshita. 2005. PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol. J. Biol. Chem. 280, 9489-9497.
14. Kinoshita, T. and N. Inoue. 2000. Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis. Curr. Opin. Chem. Biol. 4, 632-638.
15. Maeda, Y., S. Tomita, R. Watanabe, K. Ohishi, and T. Kinoshita. 1998. DPM2 regulates biosynthesis of dolichol phosphate-mannose in mammalian cells: correct subcellular localization and stabilization of DPM1, and binding of dolichol phosphate. EMBO J. 17, 4920-4929.
16. Maeda, Y., S. Tanaka, J. Hino, K. Kangawa, and T. Kinoshita. 2000. Human dolichol-phosphate-mannose synthase consists of three subunits, DPMI, DPM2 and DPM3. EMBO J. 19, 2475-2482.
17. Murakami, Y., U. Siripanyapinyo, Y. Hong, J.Y. Kang, S. Ishihara, H. Nakakuma, Y. Maeda, and T. Kinoshita. 2003. PIG-W is critical for inositol acylation but not for flipping of glycosylphosphatidylinositol-anchor. Mol. Biol. Cell. 14, 4285-4295.
18. Nagamune, K., T. Nozaki, Y. Maeda, K. Ohishi, T. Fukuma, T. Hara, R.T. Schwarz, C. Sutterlin, R. Brun, H. Riezman, and T. Kinoshita. 2000. Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei. Proc. Natl. Acad. Sci. USA 97, 10336-10341.
19. Nakamura, N., N. Inoue, R. Watanabe, M. Takahashi, J. Takeda, V.L. Stevens, and T. Kinoshita. 1997. Expression cloning of PIG-L, a candidate N-acetylglucosaminyl-phosphatidylinositol deacetylase. J. Biol. Chem. 272, 15834-15840.
20. Parker, M.W., J.T. Buckley, J.P. Postma, A.D. Tucker, K. Leonard, F. Pattus, and D. Tsernoglou. 1994. Structure of the Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states. Nature 367, 292-295.
21. Quinn, C.P., Y. Singh, K.R. Klimpel, and S.H. Leppla. 1991. Functional mapping of anthrax toxin lethal factor by in-frame insertion mutagenesis. J. Biol. Chem. 266, 20124-20130.
22. Sellman, B.R. and R.K. Tweten. 1997. The propeptide of Clostridium septicum alpha toxins functions as an intramolecular chaperone and is a potent inhibitor of alpha toxins-dependent cytolysis. Mol. Microbiol. 25, 429-440.
23. Shin, D.-J., J.J. Lee, H.E. Choy, and Y. Hong. 2004. Generation and characterization of Clostridium septicum alpha toxins mutants and their use in diagnosing paroxysmal nocturnal hemoglobinuria. Biochem. Biophys. Res. Commun. 324, 753-760.
24. Sousa, M.V., M. Richardson, W. Fontes, and L. Morhy. 1994. Homology between the seed cytolysin enterolobin and bacterial aerolysins. J. Protein Chem. 13, 659-667.
25. Takeda, J., T. Miyata, K. Kawagoe, Y. Iida, Y. Endo, T. Fujita, M. Takahashi, T. Kitani, and T. Kinoshita. 1993. Deficiency of the GPI-anchor caused by a somatic mutation of the PIG-A gene in paroxysmal nocturnal hemoglobinuria. Cell 73, 703-711.
26. Tarutani, M., S. Itami, M. Okabe, M. Ikawa, T. Tezuka, K. Yoshikawa, T. Kinoshita, and J. Takeda. 1997. Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development. Proc. Natl. Acad. Sci. USA 94, 7400-7405.
27. van der Goot, F.G., K.R. Hardie, M.W. Parker, and J.T. Buckley. 1994. The C-terminal peptide produced upon proteolytic activation of the cytolytic toxin aerolysin is not involved in channel formation. J. Biol. Chem. 269, 30496-30501.
28. Ware, F.E. and M.A. Lehrman. 1998. Expression cloning of a novel suppressor of the Lec15 and Lec35 glycosylation mutations of Chinese hamster ovary cells. J. Biol. Chem. 273, 13366.