Two stable, conducting conformations of the selectivity filter in Shaker K+ channels

Journal of General Physiology

Volumn 125, Issue 6, 2005, Pages 619-629

  Thompson, Jill ^a   Begenisich, Ted ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

Ion occupancy; K channels; Selectivity filter; Shaker; TEA

Indexed keywords

MEMBRANE PROTEIN; POTASSIUM CHANNEL; POTASSIUM CHANNEL BLOCKING AGENT;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL MEMBRANE CONDUCTANCE; CELL MEMBRANE DEPOLARIZATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CRYSTALLOGRAPHY; ELECTRIC POTENTIAL; ELECTROPHYSIOLOGY; FEMALE; MEMBRANE FILTER; NONHUMAN; PROTEIN CONFORMATION; PROTEIN STABILITY;

ALGORITHMS; ANIMALS; ELECTROPHYSIOLOGY; MEMBRANE POTENTIALS; OOCYTES; PATCH-CLAMP TECHNIQUES; POTASSIUM CHANNEL BLOCKERS; POTASSIUM CHANNELS; PROTEIN CONFORMATION; RNA; SHAKER SUPERFAMILY OF POTASSIUM CHANNELS; TETRAETHYLAMMONIUM; XENOPUS LAEVIS;

EID: 22244482453     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.200509251     Document Type: Article

References (27)

1. Armstrong, C.M., and F. Bezanilla. 1977. Inactivation of the sodium channel. II. Gating current experiments. J. Gen. Physiol. 70:567-590.
2. + channel. Science. 271:653-656.
3. Begenisich, T.B., and M.D. Cahalan. 1980. Sodium channel permeation in squid axons. I: Reversal potential experiments. J. Physiol. 307:217-242.
4. Berneche, S., and B. Roux. 2001. Energetics of ion conduction through the K channel. Nature. 414:73-77.
5. + channels. Proc. Natl. Acad. Sci. USA. 88:5092-5095.
6. + channels by TEA: results from molecular dynamics simulations of the KcsA channel. J. Gen. Physiol. 118:207-218.
7. + conduction and selectivity. Science. 280:69-77.
8. Goldin, A.L. 1992. Maintenance of Xenopus laevis and oocyte injection. Methods Enzymol. 207:266-279.
9. + channels in human T lymphocytes. Biophys. J. 55:203-206.
10. Hoshi, T., W.N. Zagotta, and R.W. Aldrich. 1990. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science. 250:533-538.
11. + channels: effects of alterations in the carboxy-terminal region. Neuron. 7:547-556.
12. +-dependent modulation of tetraethylammonium efficacy in Kv2.1 potassium channels. J. Gen. Physiol. 115:509-518.
13. Immke, D., M. Wood, L. Kiss, and S.J. Korn. 1999. Potassium-dependent changes in the conformation of the Kv2.1 potassium channel pore. J. Gen. Physiol. 113:819-836.
14. Korn, S.J., and S.R. Ikeda. 1995. Permeation selectivity by competition in a delayed rectifier potassium channel. Science. 269:410-412.
15. Kutluay, E., B. Roux, and L. Heginbotham. 2005. Rapid intracellular TEA block of the KcsA potassium channel. Biophys. J. 88:1018-1029.
16. Levy, D.I., and C. Deutsch. 1996. Recovery from C-type inactivation is modulated by extracellular potassium. Biophys. J. 70:798-805.
17. Lopez-Barneo, J., T. Hoshi, S.H. Heinemann, and R.W. Aldrich. 1993. Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels. Receptors Channels. 1:61-71.
18. + selectivity filter. Nature. 414:37-42.
19. Thompson, J., and T. Begenisich. 2000. Interaction between quaternary ammonium ions in the pore of potassium channels. Evidence against an electrostatic repulsion mechanism. J. Gen. Physiol. 115:769-782.
20. + ion binding site in shaker K channels. J. Gen. Physiol. 117:373-384.
21. + ions within the selectivity filter. J. Gen. Physiol. 122:239-246.
22. + channels. J. Physiol. 549:107-120.
23. + channel. Science. 251:939-942.
24. + channel with altered conduction properties and selectivity filter ion distribution. J. Mol. Biol. 338:839-846.
25. + selectivity filter: charge balance and coupling of ion binding to a protein conformational change underlie high conduction rates. J. Mol. Biol. 333:965-975.
26. Zhou, Y., and R. MacKinnon. 2004. Ion binding affinity in the cavity of the KcsA potassium channel. Biochemistry. 43:4978-4982.
27. + channel-Fab complex at 2.0 Å resolution. Nature. 414:43-48.