Novel method for the production of pure glycinin from soybeans

Journal of Agricultural and Food Chemistry

Volumn 53, Issue 13, 2005, Pages 5265-5269

  Golubovic, Marijana ^a   Van Hateren, Stef H ^a   Ottens, Marcel ^a   Witkamp, Geert Jan ^a   Van Der Wielen, Luuk A M ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

Carbon dioxide; Glycinin; Isoelectric precipitation; Purification; Soybean proteins

Indexed keywords

CARBON DIOXIDE; GLOBULIN; GLYCININ; SOYBEAN PROTEIN;

ARTICLE; CHEMISTRY; ISOLATION AND PURIFICATION; PH; PRECIPITATION; PRESSURE; SCANNING ELECTRON MICROSCOPY; SOLUBILITY; SOYBEAN; ULTRASTRUCTURE;

CARBON DIOXIDE; GLOBULINS; HYDROGEN-ION CONCENTRATION; MICROSCOPY, ELECTRON, SCANNING; PRECIPITATION; PRESSURE; SOLUBILITY; SOYBEAN PROTEINS; SOYBEANS;

GLYCINE MAX;

EID: 22244442710     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf0478206     Document Type: Article

References (16)

1. Liu, K. Soybeans: Chemistry, Technology and Utilization; Chapman and Hall: New York, 1997.
2. Lakemond, C. M. M.; de Jongh, H. H. J.; Paques, M.; van Vliet, T.; Gruppen, H.; Voragen, A. G. J. Gelation of soy glycinin; influence of pH and ionic strength on network structure in relation to protein conformation. Food Hydrocolloids 2003, 17, 365-377.
3. Lakemond, C. M. M.; de Jongh, H. H. J.; Gruppen, H.; Voragen, A. G. J. Differences in denaturation of genetic variants of soy glycinin. J. Agric. Food Chem. 2002, 50, 4275-4281.
4. Zhang, G. Y.; Tsunokawa, S.; Hayashi, Y.; Matsumoto, S.; Matsumura, Y.; Mori, T. Reconstitution of single molecular species from isolated subunits of glycinin. J. Am. Oil Chem. Soc. 2003, 80, 497-501.
5. Staswick, P. E.; Hermodson, M. A.; Nielsen, N. C. Identification of the cystines which link the acidic and basic components of the glycinin subunits. J. Biol. Chem. 1984, 259, 3431-3435.
6. Lalles, J. P.; Tukur, H. M.; Salgado, P.; Mills, E. N. C.; Morgan, M. R. A.; Quillien, L.; Levieux, D.; Toullec, R. Immunochemical studies on gastric and intestinal digestion of soybean glycinin and β-conglycinin in vivo. J. Agric. Food Chem. 1999, 47, 2797-2806.
7. Riblett, A. L.; Herald, T. J.; Schmidt, K. A.; Tilley, K. A. Characterization of β-conglycinin and glycinin soy protein fractions from four selected soybean genotypes. J. Agric. Food Chem. 2001, 49, 4983-4989.
8. Chan, M. Y. Y.; Bell, D. J.; Dunnill, P. The relationship between the zetapotential and the size of soya protein acid precipitate particles. Biotechnol. Bioeng. 1982, 24, 1897-1900.
9. Thanh, V. P.; Okubo, K.; Shibasaki, K. Isolation and characterization of the multiple 7S globulins of soybean proteins. Plant Physiol. 1975, 56, 19-22.
10. Thiering, R.; Hofland, G.; Foster, N.; Witkamp, G. J.; van de Wielen, L. Fractionation of soybean proteins with pressurized carbon dioxide as a volatile electrolyte. Biotechnol. Bioeng. 2001, 73, 1-11.
11. Thanh, V. P.; Shibasaki, K. Major proteins of soybean seeds. A straightforward fractionation and their characterization. J. Agric. Food Chem. 1976, 24, 1117-1121.
12. Fisher, R. R.; Glatz, C. E.; Murphy, P. A. Effects of mixing during acid addition on fractionally precipitated protein. Biotechnol. Bioeng. 1986, 28, 1056-1063.
13. Wu, S. W.; Murphy, P. A.; Johnson, L. A.; Fratzke, A. R.; Reuber, M. A. Pilot-plant fractionation of soybean glycinin and β-conglycinin. J. Am. Oil Chem. Soc. 1999, 76, 285-293.
14. Hofland, G. W.; de Rijke, A.; Thiering, R.; van der Wielen, L. A.; Witkamp, G. J. Isoelectric precipitation of soybean protein using carbon dioxide as a volatile acid. J. Chromatogr. B: Biomed. Sci. Appl. 2000, 743, 357-368.
15. Hofland, G. W.; Berkhoff, M.; Witkamp, G. J.; Van der Wielen, L. A. M. Dynamics of precipitation of casein with carbon dioxide. Int. Dairy J. 2003, 13, 685-697.
16. van Koningsveld, G. A.; Gruppen, H.; de Jongh, H. H. J.; Wijngaards, G.; van Boekel, M. A. J. S.; Walstra, P.; Voragen, A. G. J. Effects of ethanol on structure and solubility of potato proteins and the effects of its presence during the preparation of a protein isolate. J. Agric. Food Chem. 2002, 50, 2947-2956.