Use of elevated reverse transcription reaction temperatures in RT-PCR

BioTechniques

Volumn 20, Issue 5, 1996, Pages 782-783

  Freeman, Willard M ^a   Vrana, Sheila L ^a   Vrana, Kent E ^a  

^a WAKE FOREST SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL REACTION KINETICS; ENZYME KINETICS; HUMAN; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SHORT SURVEY; TEMPERATURE; TRANSCRIPTION REGULATION;

EID: 0029945224     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96205bm09     Document Type: Short Survey

References (5)

1. Mallet, F., G. Oriol, C. Mary, B. Verrier and B. Mandrand. 1995. Continuous RT-PCR using AMV-RT and Taq DNA polymerase: characterization and comparison to uncoupled procedures. BioTechniques 18:678-687.
2. Sambrook, J., E. Fritsch and T. Maniatis (Eds.) 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
3. Shimomaye, E. and M. Salvat. 1989. Use of avian myeloblastosis virus reverse transcriptase at high temperature for sequence analysis of highly structured RNA. Gene Anal. Tech. 6:25-28.
4. Vrana, S.L., B.W. Kluttz and K.E. Vrana. 1995. Application of quantitative RT-PCR to the analysis of dopamine receptor mRNA levels in rat striatum. Mol. Brain Res. 34:121-134.
5. Wang, R.F., W.W. Cao and W.G. Johnson. 1992. A simplified single buffer system for RNA-PCR. BioTechniques 12:702-704.