Probing the role of tightly bound phosphoenolpyruvate in Escherichia coli 3-deoxy-D-manno-octulosonate 8-phosphate synthase catalysis using quantitative time-resolved electrospray ionization mass spectrometry in the millisecond time range

Analytical Biochemistry

Volumn 343, Issue 1, 2005, Pages 35-47

  Li, Zhili ^a   Sau, Apurba K ^a   Furdui, Cristina M ^a   Anderson, Karen S ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

E. coli 3 deoxy D manno octulosonate 8 phosphate synthase; Electrospray ionization mass spectrometry; Phosphoenolpyruvate; Rapid chemical quench; Transient kinetics

Indexed keywords

ASSOCIATION REACTIONS; CATALYSIS; CATALYST ACTIVITY; COMPUTER SOFTWARE; ELECTROSPRAY IONIZATION; ENZYME KINETICS; ESCHERICHIA COLI; INDUCTIVELY COUPLED PLASMA; KINETICS; MASS SPECTROMETRY; PURIFICATION; REACTION INTERMEDIATES; SPECTROPHOTOMETRY; SUBSTRATES;

ELECTROSPRAY IONIZATION MASS SPECTROMETRY; INTERMEDIATE FORMATION; P-NITROPHENYL ACETATE; PHOSPHOENOLPYRUVATES; SPECTROMETRY TECHNIQUE; STOPPED-FLOW SPECTROPHOTOMETRY; SYNTHASES; TRANSIENT KINETICS;

RATE CONSTANTS;

ARABINOSE DERIVATIVE; CARBON 14; PHOSPHATE; PHOSPHOENOLPYRUVATE; SYNTHETASE;

ARTICLE; CATALYSIS; COMPARATIVE STUDY; COMPUTER PROGRAM; CONCENTRATION (PARAMETERS); ELECTROSPRAY MASS SPECTROMETRY; ENZYME ISOLATION; ESCHERICHIA COLI; FLOW KINETICS; HYDROLYSIS; ISOTOPE LABELING; KINETICS; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PURIFICATION; QUANTITATIVE ANALYSIS; SIMULATION; SPECTROPHOTOMETRY; STEADY STATE; TIME OF FLIGHT MASS SPECTROMETRY; VALIDATION PROCESS;

ESCHERICHIA COLI;

EID: 22144444949     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.04.021     Document Type: Article

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