PIASy mediates SUMO-2 conjugation of Topoisomerase-II on mitotic chromosomes

EMBO Journal

Volumn 24, Issue 12, 2005, Pages 2172-2182

  Azuma, Yoshiaki ^a   Arnaoutov, Alexei ^a   Anan, Tadashi ^a,b   Dasso, Mary ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b KUMAMOTO UNIV SCHOOL OF MEDICINE   (Japan)

Author keywords

Chromatin; Mitosis; PIASy; SUMO; Topoisomerase II

Indexed keywords

CELL PROTEIN; DNA TOPOISOMERASE (ATP HYDROLYSING); ENHANCED GREEN FLUORESCENT PROTEIN; PROTEIN INHIBITOR OF ACTIVATED STAT; PROTEIN UBC9; SUMO 2 PROTEIN; SUMO PROTEIN; UNCLASSIFIED DRUG;

ANAPHASE; ANIMAL CELL; ARTICLE; CENTROMERE; CHROMATIN; CHROMOSOME; CHROMOSOME SEGREGATION; COMPLEX FORMATION; CONJUGATION; CONTROLLED STUDY; ENZYME MODIFICATION; IN VITRO STUDY; MITOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEPLETION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; SISTER CHROMATID; WESTERN BLOTTING; XENOPUS;

ANIMALS; CHROMATIN; CHROMOSOME SEGREGATION; CHROMOSOMES; DNA TOPOISOMERASES, TYPE II; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MITOSIS; MITOTIC SPINDLE APPARATUS; PROTEIN BINDING; PROTEIN INHIBITORS OF ACTIVATED STAT; PROTEIN STRUCTURE, TERTIARY; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; UBIQUITIN-CONJUGATING ENZYMES; XENOPUS;

EID: 21844437072     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.emboj.7600700     Document Type: Article

References (25)

1. Aravind L, Koonin EV (2000) SAP-a putative DNA-binding motif involved in chromosomal organization. Trends Biochem Sci 25: 112-114
2. Arnaoutov A, Dasso M (2003) The Ran GTPase regulates kinetochore function. Dev Cell 5: 99-111
3. Ayaydin F, Dasso M (2004) Distinct in vivo dynamics of vertebrate SUMO paralogues. Mol Biol Cell 15: 5208-5218
4. Azuma Y, Arnaoutov A, Dasso M (2003) SUMO-2/3 regulates topoisomerase II in mitosis. J Cell Biol 163: 477-487
5. Azuma Y, Tan SH, Cavenagh MM, Ainsztein AM, Saitoh H, Dasso M (2001) Expression and regulation of the mammalian SUMO-1 E1 enzyme. FASEB J 15: 1825-1827
6. Bachant J, Alcasabas A, Blat Y, Kleckner N, Elledge SJ (2002) The SUMO-1 isopeptidase Smt4 is linked to centromeric cohesion through SUMO-1 modification of DNA topoisomerase II. Mol Cells 9: 1169-1182
7. Bernier-Villamor V, Sampson DA, Matunis MJ, Lima CD (2002) Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1. Cell 108: 345-356
8. Chun TH, Itoh H, Subramanian L, Iniguez-Lluhi JA, Nakao K (2003) Modification of GATA-2 transcriptional activity in endothelial cells by the SUMO E3 ligase PIASy. Circ Res 92: 1201-1208
9. Cleveland DW, Mao Y, Sullivan KF (2003) Centromeres and kinotochores: from epigenetics to mitotic checkpoint signaling. Cell 112: 407-421
10. Desai A, Murray A, Mitchison TJ, Walczak CE (1999) The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro. Methods Cell Biol 61: 385-412
11. Hari KL, Cook KR, Karpen GH (2001) The Drosophila Su(var)2-10 locus regulates chromosome structure and function and encodes a member of the PIAS protein family. Genes Dev 15: 1334-1348
12. Hauf S, Waizenegger IC, Peters JM (2001) Cohesin cleavage by separase required for anaphase and cytokinesis in human cells. Science 293: 1320-1323
13. Hochstrasser M (2001) SP-RING for SUMO: new functions bloom for a ubiquitin-like protein. Cell 107: 5-8
14. Johnson ES (2004) Protein modification by SUMO. Annu Rev Biochem 73: 355-382
15. Kornbluth S, Yang J, Powers M (2001) Analysis of the cell cycle using Xenopus egg extracts. In Current Protocols in Cell Biology, Bonifacino JS, Dasso M, Lippincott-Schwartz J, Harford JB, Yamada KM (eds), pp 11.11.11-11.11.13. New York, NY: John Wiley & Sons, Inc.
16. Luke M, Bogenhagen DF (1989) Quantitation of type II topoisomerase in oocytes and eggs of Xenopus laevis. Dev Biol 136: 459-468
17. McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT (2003) The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev 17: 101-114
18. Melchior F, Schergaut M, Pichler A (2003) SUMO: ligases, isopeptidases and nuclear pores. Trends Biochem Sci 28: 612-618
19. Minty A, Dumont X, Kaghad M, Caput D (2000) Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1 interaction motif. J Biol Chem 275: 36316-36323
20. Okubo S, Hara F, Tsuchida Y, Shimotakahara S, Suzuki S, Hatanaka H, Yokoyama S, Tanaka H, Yasuda H, Shindo H (2004) NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and A/T-rich DNA oligomers. J Biol Chem 279: 31455-31461
21. Sachdev S, Bruhn L, Sieber H, Pichler A, Melchior F, Grosschedl R (2001) PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies. Genes Dev 15: 3088-3103
22. Wong KA, Kim R, Christofk H, Gao J, Lawson G, Wu H (2004) Protein inhibitor of activated STAT Y (PIASy) and a splice variant lacking exon 6 enhance sumoylation but are not essential for embryogenesis and adult life. Mol Cell Biol 24: 5577-5586
23. Wustmann G, Szidonya J, Taubert H, Reuter G (1989) The genetics of position-effect variegation modifying loci in Drosophila melanogaster. Mol Gen Genet 217: 520-527
24. Xhemalce B, Seeler JS, Thon G, Dejean A, Arcangioli B (2004) Role of the fission yeast SUMO E3 ligase Pli1p in centromere and telomere maintenance. EMBO J 23: 3844-3853
25. Zhao X, Blobel G (2005) A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization. Proc Natl Acad Sci USA 102: 4777-4782