C/EBPα activates the transcription of triacylglycerol hydrolase in 3T3-L1 adipocytes

Biochemical Journal

Volumn 388, Issue 3, 2005, Pages 959-966

  Wei, Enhui ^a   Lehner, Richard ^a,b   Vance, Dennis E ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b CANADIAN INSTITUTES OF HEALTH RESEARCH   (Canada)

Author keywords

3T3 L1 adipocyte; CCAAT enhancer binding protein (C EBP ); Lipolysis; NIH 3T3 cell; Transient transfection; Triacylglycerol hydrolase

Indexed keywords

BIOASSAY; CATALYSIS; DNA; ELECTROPHORESIS; GLYCEROL; MUTAGENESIS; RNA; TISSUE;

ADIPOCYTES; LIPOLYSIS; SUPERSHIFT ASSAYS; TRIACYLGLYCEROL HYDROLASE (TGH);

ENZYMES;

CCAAT ENHANCER BINDING PROTEIN ALPHA; COMPLEMENTARY DNA; NUCLEAR PROTEIN; TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE;

ADIPOCYTE; ADIPOSE TISSUE; ANIMAL CELL; ARTICLE; CELL MIGRATION; CELL STRAIN 3T3; CONTROLLED STUDY; GEL MOBILITY SHIFT ASSAY; GENETIC TRANSCRIPTION; LIPOLYSIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROADIPOCYTE; TRANSCRIPTION REGULATION; ANIMAL; ANTIBODY SPECIFICITY; BINDING SITE; CELL DIFFERENTIATION; CELL LINE; CERCOPITHECUS; DNA RESPONSIVE ELEMENT; ENZYME INDUCTION; GENETIC TRANSFECTION; GENETICS; METABOLISM; PROMOTER REGION; PROTEIN BINDING;

MURINAE;

3T3-L1 CELLS; ADIPOCYTES; ANIMALS; BINDING SITES; CCAAT-ENHANCER-BINDING PROTEIN-ALPHA; CELL DIFFERENTIATION; CELL LINE; CERCOPITHECUS AETHIOPS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; ENZYME INDUCTION; LIPASE; MICE; ORGAN SPECIFICITY; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; RESPONSE ELEMENTS; TRANSCRIPTION, GENETIC; TRANSFECTION; TRIGLYCERIDES;

EID: 21744443444     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20041442     Document Type: Article

References (36)

1. Londos, C., Brasaemle, D. L., Schultz, C. J., Adler-Wailes, D. C., Levin, D. M., Kimmel, A. R. and Rondinone, C. M. (1999) On the control of lipolysis in adipocytes. Ann. N.Y. Acad. Sci. 892, 155-168
2. Krey, G., Braissant, O., L'Horset, F., Kalkhoven, E., Perroud, M., Parker, M. G. and Wahli, W. (1997) Fatty acids, eicosanoids, and hypolipidemic agents identified as ligands of peroxisome proliferator-activated receptors by coactivator-dependent receptor ligand assay. Mol. Endocrinol. 11, 779-791
3. Holm, C., Kirchgessner, T. G., Svenson, K. L., Fredrikson, G., Nilsson, S., Miller, C. G., Shively, J. E., Heinzmann, C., Sparkes, R. S., Mohandas, T. et al. (1988) Hormone-sensitive lipase: sequence, expression, and chromosomal localization to 19 cent-q13.3. Science 241, 1503-1506
4. Carey, G. B. (1998) Mechanisms regulating adipocyte lipolysis. Adv. Exp. Med. Biol. 441, 157-170
5. Okazaki, H., Osuga, J., Tamura, Y., Yahagi, N., Tomita, S., Shionoiri, F., Iizuka, Y., Ohashi, K., Harada, K., Kimura, S. et al. (2002) Lipolysis in the absence of hormone-sensitive lipase: evidence for a common mechanism regulating distinct lipases. Diabetes 51, 3368-3375
6. Osuga, J., Ishibashi, S., Oka, T., Yagyu, H., Tozawa, R., Fujimoto, A., Shionoiri, F., Yahagi, N., Kraemer, F. B., Tsutsumi, O. and Yamada, N. (2000) Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy, but not in obesity. Proc. Natl. Acad. Sci. U.S.A. 97, 787-792
7. Haemmerle, G., Zimmermann, R., Strauss, J. G., Kratky, D., Riederer, M., Knipping, G. and Zechner, R. (2002) Hormone-sensitive lipase deficiency in mice changes the plasma lipid profile by affecting the tissue-specific expression pattern of lipoprotein lipase in adipose tissue and muscle. J. Biol. Chem. 277, 12946-12952
8. Dolinsky, V. W., Sipione, S., Lehner, R. and Vance, D. E. (2001) The cloning and expression of a murine triacylglycerol hydrolase cDNA and the structure of its corresponding gene. Biochim. Biophys. Acta 1532, 162-172
9. Lehner, R. and Vance, D. E. (1999) Cloning and expression of a cDNA encoding a hepatic microsomal lipase that mobilizes stored triacylglycerol. Biochem. J. 343, 1-10
10. Gilham, D., Ho, S., Rasouli, M., Martres, P., Vance, D. E. and Lehner, R. (2003) Inhibitors of hepatic microsomal triacylglycerol hydrolase decrease very low density lipoprotein secretion. FASEB J. 17, 1685-1687
11. Soni, K. G., Lehner, R., Metalnikov, P., O'Donnell, P., Semache, M., Gao, W., Ashman, K., Pshezhetsky, A. V. and Mitchell, G. A. (2004) Carboxylesterase 3 (EC 3.1.1.1) is a major adipocyte lipase. J. Biol. Chem. 279, 40683-40689
12. Dolinsky, V. W., Gilham, D., Hatch, G. M., Agellon, L. B., Lehner, R. and Vance, D. E. (2003) Regulation of triacylglycerol hydrolase expression by dietary fatty acids and peroxisomal proliferator-activated receptors. Biochim. Biophys. Acta 1635, 20-28
13. Douglas, D. N., Dolinsky, V. W., Lehner, R. and Vance, D. E. (2001) A role for Sp1 in the transcriptional regulation of hepatic triacylglycerol hydrolase in the mouse. J. Biol. Chem. 276, 25621-25630
14. Lin, F. T. and Lane, M. D. (1994) CCAAT/enhancer binding protein alpha is sufficient to initiate the 3T3-L1 adipocyte differentiation program. Proc. Natl. Acad. Sci. U.S.A. 91, 8757-8761
15. Deryckere, F. and Gannon, F. (1994) A one-hour minipreparation technique for extraction of DNA-binding proteins from animal tissues. Biotechniques 16, 405
16. Ntambi, J. M. and Young-Cheul, K. (2000) Adipocyte differentiation and gene expression. J. Nutr. 130, 3122S-3126S
17. Camp, H. S., Ren, D. and Leff, T. (2002) Adipogenesis and fat-cell function in obesity and diabetes. Trends Mol. Med. 8, 442-447
18. Christy, R. J., Yang, V. W., Ntambi, J. M., Geiman, D. E., Landschulz, W. H., Friedman, A. D., Nakabeppu Y., Kelly, T. J. and Lane, M. D. (1989) Differentiation-induced gene expression in 3T3-L1 preadipocytes: CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes. Genes Dev. 3, 1323-1335
19. Ross, S. E., Erickson, R. L., Gerin, I., DeRose, P. M., Bajnok, L., Longo, K. A., Misek, D. E., Kuick, R., Hanash, S. M., Atkins, K. B. et al. (2002) Microarray analyses during adipogenesis: understanding the effects of Wnt signaling on adipogenesis and the roles of liver X receptor alpha in adipocyte metabolism. Mol. Cell. Biol. 22, 5989-5999
20. Millet, S. G., De Vos, P., Guerre-Millo, M., Wong, K., Hermann, T., Staels, B., Briggs, M. R. and Auwerx, J. (1996) The adipocyte specific transcription factor C/EBPalpha modulates human ob gene expression. Proc. Natl. Acad. Sci. U.S.A. 93, 5507-5511
21. Zhang, J. W., Klemm, D. J., Vinson, C. and Lane, M. D. (2004) Role of CREB in transcriptional regulation of CCAAT/enhancer-binding protein beta gene during adipogenesis. J. Biol. Chem. 279, 4471-4478
22. Tang, Q. Q. and Lane, M. D. (1999) Activation and centromeric localization of CCAAT/enhancer-binding proteins during the mitotic clonal expansion of adipocyte differentiation. Genes Dev. 13, 2231-2241
23. Lane, M. D., Tang, Q. Q. and Jiang, M. S. (1999) Role of the CCAAT enhancer binding proteins (C/EBPs) in adipocyte differentiation. Biochem. Biophys. Res. Commun. 266, 677-683
24. Zhu, Q., Zhu, J. and Liao, K. (2000) An SP1-like cis-element is the major DNA motif for differential expression regulation of the adipocyte amino acid transporter. Biochem. Biophys. Res. Commun. 271, 91-99
25. Barth, N., Langmann, T., Scholmerich, J., Schmitz, G. and Schaffler, A. (2002) Identification of regulatory elements in the human adipose most abundant gene transcript-1 (apM-1) promoter: role of SP1/SP3 and TNF-alpha as regulatory pathways. Diabetologia 45, 1425-1433
26. Harp, J. B., Franklin, D., Vanderpuije, A. A. and Gimble, J. M. (2001) Differential expression of signal transducers and activators of transcription during human adipogenesis. Biochem. Biophys. Res. Commun. 281, 907-912
27. Nanbu-Wakao, R., Morikawa, Y., Matsumura, I., Masuho, Y., Muramatsu, M. A., Senba, E. and Wakao, H. (2002) Stimulation of 3T3-L1 adipogenesis by signal transducer and activator of transcription 5. Mol. Endocrinol. 16, 1565-1576
28. Floyd, Z. E. and Stephens, J. M. (2003) STAT5A promotes adipogenesis in nonprecursor cells and associates with the glucocorticoid receptor during adipocyte differentiation. Diabetes 52, 308-314
29. Lehner, R. and Verger, R. (1997) Purification and characterization of a porcine liver microsomal triacylglycerol hydrolase. Biochemistry 36, 1861-1868
30. Brun, R. P. and Spiegelman, B. M. (1997) PPAR gamma and the molecular control of adipogenesis. J. Endocrinol. 155, 217-218
31. Suzawa, M., Takada, I., Yanagisawa, J., Ohtake, F., Ogawa, S., Yamauchi, T., Kadowaki, T., Takeuchi, Y., Shibuya, H., Gotoh, Y. et al. (2003) Cytokines suppress adipogenesis and PPAR-gamma function through the TAK1/TAB1/NIK cascade. Nat. Cell Biol. 5, 224-230
32. Seo, J. B., Moon, H. M., Noh, M. J., Lee, Y. S., Jeong, H. W., Yoo, E. J., Kim, W. S., Park, J., Youn, B. S., Kim, J. W. et al. (2004) Adipocyte determination- and differentiation-dependent factor 1/sterol regulatory element-binding protein 1c regulates mouse adiponectin expression. J. Biol. Chem. 279, 22108-22117
33. MacDougald, O. A., Cornelius, P., Liu, R. and Lane, M. D. (1995) Insulin regulates transcription of the CCAAT/enhancer binding protein (C/EBP) alpha, beta, and delta genes in fully-differentiated 3T3-L1 adipocytes. J. Biol. Chem. 270, 647-654
34. Hemati, N., Ross, S. E., Erickson, R. L., Grobiewski, G. E. and MacDougald, O. A. (1997) Signaling pathways through which insulin regulates CCAAT/enhancer binding protein alpha (C/EBPalpha) phosphorylation and gene expression in 3T3-L1 adipocytes. Correlation with GLUT4 gene expression. J. Biol. Chem. 272, 25913-25919
35. Diehl, A. M., Michaelson, P. and Yang, S. Q. (1994) Selective induction of CCAAT/enhancer binding protein isoforms occurs during rat liver development. Gastroenterology 106, 1625-1637
36. Lehner, R., Cui, Z. and Vance, D. E. (1999) Subcellullar localization, developmental expression and characterization of a liver triacylglycerol hydrolase. Biochem. J. 338, 761-768