High-level expression of recombinant phospholipase C from Bacillus cereus in Pichia pastoris and its characterization

Biotechnology Letters

Volumn 26, Issue 19, 2004, Pages 1475-1479

  Seo, Kook Hwa ^a   Rhee, Jong Il ^a  

^a Chonnam National University   (South Korea)

Author keywords

Bacillus cereus; Characterization; Expression; Phospholipase C; Pichia pastoris

Indexed keywords

ACETONE; BACTERIA; COBALT COMPOUNDS; ENZYME KINETICS; METHANOL; ORGANIC SOLVENTS; PH EFFECTS; PHOSPHATES; PROTEINS; SURFACE ACTIVE AGENTS;

BACILLUS CEREUS; HIGH-LEVEL EXPRESSION; PHOSPHOLIPASE C; PICHIA PASTORIS;

GENES;

BACILLUS CEREUS; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

PHOSPHOLIPASE C; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS CEREUS; BIOSYNTHESIS; CHEMISTRY; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMOLOGY; GENETIC TRANSFORMATION; GENETICS; METHODOLOGY; MOLECULAR WEIGHT; PH; PICHIA; PROTEIN ENGINEERING; TEMPERATURE;

BACILLUS CEREUS; ENZYME ACTIVATION; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; PHOSPHOLIPASE C; PICHIA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TEMPERATURE; TRANSFORMATION, GENETIC;

EID: 21644464966     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1023/B:BILE.0000044447.15205.90     Document Type: Article

References (10)

1. Anthonsen T, D'Arrigo P, Pedrocchi-Fantoni G, Secundo F, Servi S, Sundby E (1999) Phospholipids hydrolysis in organic solvents catalysed by immobilized phospholipase C. J. Mol. Cat. B; Enzym. 6: 125-132.
2. Bornscheuer U, Reif O-W, Lausch R, Freitag R, Scheper T, Kolisis EN, Menge U (1994) Lipase of Pseudomonas cepacia for biotechnological purposes: purification, crystallization and characterization. Biochim. Biophys. Acta 1201: 55-60.
3. Hergenrother PJ, Martin SF (2000) Phosphatidylcholine-preferring phospholipase C from B. cereus: function, structure, and mechanism. Topics Curr. Chem. 211: 131-167.
4. Kurioka S, Matsuda M (1976) Phospholipase C assay using p-nitrophenyl-phosphorylcholine together with sorbital and its application to studying the metal and detergent requirement of the enzyme. Anal. Biochem. 75: 281-289.
5. Levine L, Xiao D, Little C (1998) Increased arachidonic acid metabolites from cells in culture after treatment with the phosphatidylcholine-hydrolyzing phospholipase C from Bacillus cereus. Prostaglandins 34: 633-642.
6. Martin SF, Follows BC, Hergenrother PJ, Trotter BK (2000) The choline binding site of phospholipase C (Bacillus cereus): insights into substrate specificity. Biochemistry 39: 3410-3415.
7. Minning S, Schmidt-Dannert C, Schmid RD (1998) Functional expression of Rhizopus oryzae lipase in Pichia pastoris: high-level production and some properties. J. Biotechnol. 66: 147-156.
8. Quyen DT, Claudia SD, Schmid RD (2003) High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichia pastoris and some properties of the recombinant lipase. Protein Expr. Purif. 28: 102-110.
9. Sambrook J, Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. New York: Cold Spring Harbor Laboratory Press.
10. Tan CA, Hehir MJ, Roberts MF (1997) Cloning, overexpression, refolding, and purification of the nonspecific phospholipase C from Bacillus cereus. Protein Expr. Purif. 10: 365-372.