Monomeric B27 Lys destripeptide insulin: Semisynthesis, characterization and biological activity

Acta Biochimica et Biophysica Sinica

Volumn 35, Issue 3, 2003, Pages 215-218

  Ding, Jin Guo ^a   Cui, Da Fu ^a   Zhang, You Shang ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

B27 Lys destripeptide insulin; Biological activity; Monomeric insulin

Indexed keywords

INSULIN; INSULIN DERIVATIVE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; DIABETES MELLITUS; DRUG ACTIVITY; DRUG HYDROLYSIS; DRUG RECEPTOR BINDING; DRUG SYNTHESIS; GEL FILTRATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VIVO STUDY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECURSOR; SACCHAROMYCES CEREVISIAE; THIN LAYER CHROMATOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; BINDING, COMPETITIVE; BLOOD GLUCOSE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, THIN LAYER; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYPOGLYCEMIC AGENTS; INSULIN; MICE; PEPTIDE FRAGMENTS; RECEPTOR, INSULIN; SWINE;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0038559578     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Insulin research group. Structural studies on des-pentapeptide (B26 - 30)-insulin III state of aggregation, tyrosyl environment, and peptide backbone conformation in solution. Sci Sin, 1976, 19: 475-485
2. Cao QP, Cui DF, Zhang YS. Enzymatic synthesis of deshexapeptide insulin. Nature, 1981, 292: 774-775
3. Shvachkin IuP, Shmeleva GA, Krivtsov VF, Fedotov VP, Ivanova AI. Preparation and properties of des-(pentapeptide-B26-30)-insulin. Biokhimiia, 1972, 37: 966-973
4. Brems DN, Alter LA, Beckage MJ, Chance RE, DiMarchi RD, Green LK, Long HB et al. Altering the association properties of insulin by amino acid replacement. Protein Eng, 1992, 5: 527-533
5. Cui DF, Li MY, Zhang YS, Feng YM. Monomeric destetrapeptide human insulin from a precursor expressed in Saccharomyces cerevisiae. J Pept Res, 2001, 57: 188-192
6. Gabriel O. Analytical disc gel electrophoresis. In: Jakoby WB ed. Mehtods in Enzymology, New York: Academic Press, 1971, 22: 656
7. Feng YM, Zhu JH, Zhang XT, Zhang YS. Studies on the mechanism of insulin action. Acta Biochim Biophys Sin, 1982, 14: 137-143
8. Zhu SQ, Cui DF, Fan L, Huang YD, Zhang YS. Despentapeptide (B26 - 30) insulin amide: An insulin analogue with higher receptor binding capacity than native insulin. Acta Biochim Biophys Sin, 1988, 20: 292-297
9. Cui DF, Cao QP, Zhu SQ, Zhang XT, Zhang YS. Enzymatic synthesis of deshexapeptide insulin and its analogues. Sci Sin [B], 1983, 26: 248-254
10. Vajo Z, Duckworth WC. Genetically engineered insulin analogs: Diabetes in the new millenium. Pharmacol Rev, 2000, 52: 1-9
11. Brange J, Volund A. Insulin analogs with improved pharmacokinetic profiles. Adv Drug Deliv Rev, 1999, 35: 307-335