Phosphatase-1 and -2A inhibition modulates apoptosis in human osteoarthritis chondrocytes independently of nitric oxide production

Annals of the Rheumatic Diseases

Volumn 64, Issue 7, 2005, Pages 1079-1082

  Lopez Armada M J ^a   Carames B ^a   Cillero Pastor, B ^a   Lires Dean M ^a   Maneiro, E ^a   Fuentes, I ^b   Ruiz C ^a   Galdo, F ^a,b   Blanco, F J ^a  

^a HOSPITAL JUAN CANALEJO   (Spain)

^b UNIVERSITY OF A CORUÑA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BENZYLOXYCARBONYLASPARTYLGLUTAMYLVALYLASPARTYL FLUOROMETHYL KETONE; CASPASE INHIBITOR; GENOMIC DNA; INDUCIBLE NITRIC OXIDE SYNTHASE; NITRIC OXIDE; OKADAIC ACID; PHOSPHOPROTEIN PHOSPHATASE 1; PHOSPHOPROTEIN PHOSPHATASE 2A; PHOSPHOPROTEIN PHOSPHATASE INHIBITOR;

APOPTOSIS; ARTHRITIS; ARTICLE; CARTILAGE CELL; CELL CULTURE; CELL VIABILITY; CONTROLLED STUDY; DNA DAMAGE; DOSE TIME EFFECT RELATION; DRUG TARGETING; ENZYME INHIBITION; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PHOSPHORYLATION; FEMUR HEAD; HUMAN; HUMAN CELL; OSTEOARTHRITIS; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

AMINO ACID CHLOROMETHYL KETONES; APOPTOSIS; CARTILAGE, ARTICULAR; CASPASES; CELL CULTURE TECHNIQUES; CHONDROCYTES; DNA FRAGMENTATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IN SITU NICK-END LABELING; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; NITRIC OXIDE SYNTHASE TYPE II; OKADAIC ACID; OLIGOPEPTIDES; OSTEOARTHRITIS, HIP; PHOSPHOPROTEIN PHOSPHATASE;

EID: 21344467473     PISSN: 00034967     EISSN: None     Source Type: Journal    
DOI: 10.1136/ard.2004.034090     Document Type: Article

References (15)

1. Honkanen RE, Goleen T. Regulators of serine/threonine protein phosphatases at the dawn of a clinical era? Curr Med Chem 2002;9:2055-75.
2. Klumpp S, Krieglstein J. Serine/threonine protein phosphatases in apoptosis. Curr Opin Pharmacol 2002;2:458-62.
3. Zakany R, Szucs K, Bako E, Felszeghy S, Czifra G, Biro T, et al. Protein phosphatase 2A is involved in the regulation of protein kinase A signaling pathway during in vitro chondrogenesis. Exp Cell Res 2002;275:1-8.
4. Miller C, Zhang M, He Y, Zhao J, Pelletier JP, Martel-Pelletier J, et al. Transcriptional induction of cyclooxygenase-2 gene by okadaic acid inhibition of phosphatase activity in human chondrocytes: co-stimulation AP-1 and CRE nuclear binding proteins. J Cell Biochem 1998;69:392-413.
5. Blanco FJ, Guitian R, Vázquez-Martul E, J de Toro FJ, Galdo F. Osteoarthritis chondrocytes die by apoptosis. Arthritis Rheum 1998;41:284-9.
6. Maneiro E, Martín MA, De Andrés MC, López-Armada MJ, Fernandez-Sueiro JL, Del Hoyo P, et al. Mitochondrial respiratory activity is altered in OA human articular chondrocytes. Arthritis Rheum 2003;48:700-8.
7. Blanco FJ, Ochs RL, Schwarz H, Lotz M. Chondrocyte apoptosis induced by nitric oxide. Am J Pathol 1995;146:1-11.
8. Blanco FJ, Lotz M. IL-1 induced nitric oxide inhibits chondrocyte proliferation via PGE2. Exp Cell Res 1995;218:319-25.
9. Kolb TM, Chang SH, Davis MA. Biochemical and morphological events during okadaic acid-induced apoptosis of Tsc2-null ERC-18 cell line. Toxicol Pathol 2002;30:235-46.
10. Romano E, Cannata S, Di Bartalameo S, Spinedi A. Caspase inhibition shifts neuroepithelioma cell response to akadaic acid from apoptosis to an apoptotic-like form of death. Biochem Biophys Res Commun 2003;303:469-74.
11. Huynh-Delerme C, Fessard V, Kiefer-Biasizzo H, Puiseux-Dao S. Characteristics of okadaic acid-induced cytotoxic effects in CHO K1 cells. Environ Toxicol 2003;18:383-94.
12. Liu W, Akhand AA, Takeda K, Kawamoto Y, Itoigawa T, Kato M, et al. Protein phosphatase 2A-linked and -unlinked caspase-dependent pathways for downregulation of Akt kinase triggered by 4-hydroxynanenal. Cell Death Differ 2003;10:772-81.
13. Miskolci V, Castro-Alcaraz S, Nguyen P, Vancura A, Davidson D, Vancurova I. Okadaic acid induces sustained activation of NfkappaB and degradation of the nuclear IkappaBalpha in human neutrophils. Arch Biochem Biophys 2003;417:44-52.
14. Pahan K, Sheikh FG, Namboodiri AM, Singh I. Inhibitors of protein phosphatase 1 and 2A differentially regulate the expression of inducible nitric-oxide synthase in rat astrocytes and macrophages. J Biol Chem 1998;273:12219-26.
15. Sandal T, Aumo L, Hedin L, Gjertsen BT, Doskeland SO. Irod/Ian5: an inhibitor of γ-radiation- and okadaic acid-induced apoptosis. Mol Biol Cell 2003;14:3292-304.