Microbiology: Community proteomics of a natural microbial biofilm

Science

Volumn 308, Issue 5730, 2005, Pages 1915-1920

  Ram, Rachna J ^a   VerBerkmoes, Nathan C ^b   Thelen, Michael P ^a,c   Tyson, Gene W ^a   Baker, Brett J ^a   Blake II, Robert C ^d   Shah, Manesh ^b   Hettich, Robert L ^b   Banfield, Jillian F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b OAK RIDGE NATIONAL LABORATORY   (United States)

^c LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^d XAVIER UNIVERSITY OF LOUISIANA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENES; MASS SPECTROMETRY; MICROORGANISMS; OXIDATION; PROTEINS; STRESSES;

ACID MINE DRAINAGE (AMD); CYTOCHROMES; OXIDATIVE STRESSES; PROTEOMICS;

BIOFILMS;

BACTERIAL PROTEIN; CYTOCHROME; IRON; AMINO ACID; ARCHAEAL PROTEIN; PROTEOME;

MICROBIOLOGY;

ACID MINE DRAINAGE; AMINO ACID SEQUENCE; ARCHAEBACTERIUM; ARTICLE; BACTERIAL GENE; BIOFILM; BIOGEOCHEMISTRY; CELLULAR DISTRIBUTION; ENZYME ACTIVITY; FERROPLASMA ACIDARMANUS; GENE EXPRESSION; GENE FUNCTION; GENOMICS; LEPTOSPIRA; LEPTOSPIRILLUM; MASS SPECTROMETRY; MICROFLORA; NONHUMAN; OXIDATION; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEOMICS; SEQUENCE HOMOLOGY; SULFOBACILLUS; SULFUR BACTERIUM; SURVIVAL RATE; TECHNIQUE; BACTERIAL GENOME; BACTERIUM; CHEMISTRY; ECOSYSTEM; GENE; GENETICS; GENOME; GROWTH, DEVELOPMENT AND AGING; ISOELECTRIC POINT; METABOLISM; MINING; MOLECULAR GENETICS; OXIDATION REDUCTION REACTION; PH; PHYSIOLOGY; PROTEIN SYNTHESIS; THERMOPLASMALES;

ARCHAEA; FERROPLASMA ACIDARMANUS; LEPTOSPIRA; LEPTOSPIRILLUM; SULFOBACILLUS;

AMINO ACIDS; ARCHAEAL PROTEINS; BACTERIA; BACTERIAL PROTEINS; BIOFILMS; CYTOCHROMES; ECOSYSTEM; GENE EXPRESSION; GENES, ARCHAEAL; GENES, BACTERIAL; GENOME, ARCHAEAL; GENOME, BACTERIAL; GENOMICS; HYDROGEN-ION CONCENTRATION; IRON; ISOELECTRIC POINT; MASS SPECTROMETRY; MINING; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PROTEIN BIOSYNTHESIS; PROTEIN FOLDING; PROTEOME; PROTEOMICS; THERMOPLASMALES;

EID: 21344465408     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.     Document Type: Article

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32. We thank T. Arman (owner. Iron Mountain mines), R. Sugarek, D. Dodds, and R. Carver for mine access and on-site assistance. We thank D. Tabb, the Yates Laboratory, the Institute for Systems Biology for software, F. Larimer for computer access, U. Kappler for heme-staining protocols; W. Sand for strain P3A, E. Allen for help with genomic analysis, J. Flanagan for genomic DNA, and R. Whitaker and G. Fox for their comments on the manuscript. Research was supported by grants from the U.S. Department of Energy (DOE) Microbial Genome Program (J.F.B.), NSF Brocomplexity Program (J.F.B.), NASA Astrobiology Institute (J.F.B.), DOE Energy Biosciences Program (R.C.B.), and DOE Genomes to Life Program (R.H.). DDBJ/EMBL/GenBank accession numbers for the community genomic data are AADL01000000 and AADL00000000. All data, databases, and resulting identifications are available online; see (31).