A comparison of immobilized pH gradient isoelectric focusing and strong-cation-exchange chromatography as a first dimension in shotgun proteomics

Proteomics

Volumn 5, Issue 1, 2005, Pages 24-34

  Essader, Amal S ^a   Cargile, Benjamin J ^a   Bundy, Jonathan L ^a   Stephenson Jr , James L ^a  

^a RTI INTERNATIONAL   (United States)

Author keywords

Immobilized pH gradient; Isoelectric focusing; Mass spectrometry; Protein identification; Two dimensional chromatography

Indexed keywords

PEPTIDE; PROTEIN; TRYPSIN;

ALGORITHM; ANIMAL TISSUE; ARTICLE; ENZYMATIC DEGRADATION; IMMOBILIZATION; IMMOBILIZED PH GRADIENT ISOELECTRIC FOCUSING; INFORMATION PROCESSING; INTERMETHOD COMPARISON; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC FOCUSING; MALE; NONHUMAN; OFFLINE ANALYSIS; PEPTIDE ANALYSIS; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; RAT; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; TANDEM MASS SPECTROMETRY; TESTIS;

ANIMALS; CHROMATOGRAPHY, ION EXCHANGE; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; MALE; MASS SPECTROMETRY; PEPTIDES; PROTEINS; PROTEOMICS; RATS; TESTIS;

ANIMALIA; RATTUS; RATTUS NORVEGICUS;

EID: 13244271374     PISSN: 16159853     EISSN: None     Source Type: Journal    
DOI: 10.1002/pmic.200400888     Document Type: Article

References (25)

1. Peng, J., Gygi, S. P., J. Mass Spectrom. 2001, 36, 1083-1091.
2. Aebersold, R., Goodlett, D. R., Chem. Rev. 2001, 101, 269-295.
3. Ferguson, P. L., Smith, R. D., Annu. Rev. Biophys. Biomol. Struct. 2003, 32, 399-424.
4. Dreger, M., Mass Spectrom. Rev. 2003, 22, 27-56.
5. Hubbard, M. J., Proteomics 2002, 2, 1069-1078.
6. Wu, C. C., MacCoss, M. J., Curr. Opin. Mol. Ther. 2002, 4, 242-250.
7. Link, A. J., Eng, J., Schieltz, D. M., Carmack, E. et al., Nat. Biotechnol. 1999, 17, 676-682.
8. Washburn, M. P., Wolters, D., Yates III, J. R., Nat. Biotechnol. 2001, 19, 242-247.
9. Wolters, D. A., Washburn, M. P., Yates III, J. R., Anal. Chem. 2001, 73, 5683-5690.
10. Peng, J., Ellas, J. E., Thoreen, C. C., Licklider, L. J., Gygi, S. P., J. Proteome Res. 2003, 2, 43-50.
11. Cargile, B. J., Bundy, J. L., Freeman, T. W., Stephenson, J. L. Jr., J. Proteome Res. 2004, 3, 112-119.
12. Cargile, B. J., Talley, D. L., Stephenson, J. J. L., Electrophoresis 2004, 25, 936-945.
13. Chen, J. Z., Balgley, B. M., DeVoe, D. L., Lee, C. S., Anal. Chem. 2003, 75, 3145-3152.
14. Chen, J., Lee, C. S., Shen, Y., Smith, R. D., Baehrecke, E. H., Electrophoresis 2002, 23, 3143-3148.
15. Figeys, D., van Oostveen, I., Ducret, A., Aebersold, R., Anal. Chem. 1996, 68, 1822-1828.
16. Tong, W., Link, A., Eng, J. K., Yates III, J. R., Anal. Chem. 1999, 71, 2270-2278.
17. Choudary, G., Horvath, C., in: Karger, B. L., Hancock, W. S. (Eds.), Methods in Enzymology: High-resolution Separation and Analysis of Biological Macromolecules. Part A, Fundamentals, Vol. 270, Academic Press, San Diego, CA 1996, pp. 47-81.
18. Bjellqvist, B., Ek, K., Righetti, P. G., Gianazza, E. et al., J. Biochem. Biophys. Methods 1982, 6, 317-339.
19. Bjellqvist, B., Pasquali, C., Ravier, F., Sanchez, J. C., Hochstrasser, D., Electrophoresis 1993, 14, 1357-1365.
20. Görg, A., Boguth, G., Obermaier, C., Posch, A., Weiss, W., Electrophoresis 1995, 16, 1079-1086.
21. Corbett, J. M., Dunn, M. J., Posch, A., Görg, A., Electrophoresis 1994, 15, 1205-1211.
22. Eng, J. K., McCormack, A. L., Yates III, J. R., J. Am. Soc. Mass Spectrom. 1994, 5, 976-989.
23. Bjellqvist, B., Hughes, G. J., Pasquali, C., Paquet, N., Ravier, F., Sanchez, J. C., Frutiger, S., Hochstrasser, D., Electrophoresis 1993, 14, 1023-1031.
24. Rappsilber, J., Mann, M., Trends Biochem. Sci. 2002, 27, 74-78.
25. Cargile, B. J., Stephenson, J. L. Jr., Anal. Chem. 2004, 76, 267-275.