Emulsan quantitation by Nile red quenching fluorescence assay

Applied Microbiology and Biotechnology

Volumn 67, Issue 6, 2005, Pages 767-770

  Castro, Guillermo R ^a,b   Larson, Bridget K ^a   Panilaitis, Bruce ^a   Kaplan, David L ^a  

^a Tufts University   (United States)

^b PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLÓGICOS PROIMI CONICET   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

ADSORPTION; BACTERIA; FLUORESCENCE; QUENCHING;

EMULSAN; EMULSAN QUANTITATION; MOLAR EXTINCTION COEFFICIENT; NILE RED QUENCHING FLUORESCENCE ASSAY;

BIOASSAY;

9 DIETHYLAMINO 5H BENZO[A]PHENOXAZIM 5 ONE; DIMETHYL SULFOXIDE; EMULSAN; EMULSIFYING AGENT; FLUORESCENT DYE; UNCLASSIFIED DRUG;

FLUORESCENCE;

ACINETOBACTER; ADSORPTION; ARTICLE; ASSAY; BACTERIUM CULTURE; CONTROLLED STUDY; DISSOLUTION; FLUORESCENCE ANALYSIS; NONHUMAN; PHOTOCHEMICAL QUENCHING; QUANTITATIVE ANALYSIS;

ACINETOBACTER; EVALUATION STUDIES; FLUORESCENCE; OXAZINES; POLYSACCHARIDES, BACTERIAL; SPECTROMETRY, FLUORESCENCE;

ACINETOBACTER VENETIANUS;

EID: 21344438429     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-004-1849-2     Document Type: Article

References (14)

1. Carmichael AJ, Seddon KR (2000) Polarity study of some 1-alkyl-3-methylimidazolium ambient-temperature ionic liquids with the solvatochromic dye, Nile red. J Phys Org Chem 13:591-595
2. Choi MD, Kim JK, Kang TJ, Jeoung JC, Kim D (1997) Fluorescence anisotropy of Nile red and oxazine 725 in an isotropic liquid crystal. J Phys Chem B 101:8092-8097
3. Daban JR, Sansó M, Batolomé S (1991) Use of Nile red as a fluorescent probe for the study of the hydrophobia properties of protein-sodium dodecyl sulfate complexes in solution. Anal Biochem 199:162-168
4. Deye JF, Berger TA, Anderson AG (1990) Nile red as a solvatochromic dye for measuring solvent strength in normal liquids and mixtures of normal liquids with supercritical and near critical fluids. Anal Chem 62:615-622
5. Dutta AK, Kamada K, Ohta K (1996) Spectroscopic studies of nile red in organic solvents and polymers. J Photochem Photobiol A Chem 93:57-64
6. Greenspan P, Fowler SD (1985) Spectrofluorometric studies of the lipid probe, Nile red. J Lipid Res 26:781-789
7. Gorenflo V, Steinbüchel A, Marose S, Rieseberg M, Scheper T (1999) Quantification of bacterial polyhydroxyalkanoic acids by Nile red staining. Appl Microbiol Biotechnol 51:765-772
8. Johri AK, Blank W, Kaplan DL. (2002) Bioengineered emulsans from Acinetobacter calcoaceticus RAG-1 transposon mutants. Appl Microbiol Biotechnol 59:217-223
9. Panilaitis B, Johri A, Blank W, Kaplan D, Fuhrman J (2002) Adjuvant activity of emulsan, a secreted lipopolysaccharide from Acinetobacter calcoaceticus. Clin Diagn Lab Immuno 9:1240-1247
10. Rosenberg E, Ron EZ (2002) Bioemulsans: surface-active polysaccharide-containing complexes. In: Vandamme E, De Baets S, Steinbüchel A (eds) Biopolymer - polysaccharides. I. Polysaccharides from prokaryotes, vol 5. Wiley, VCH, Berlin, pp 91-112
11. Sackett DL, Wolf J (1987) Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces. Anal Biochem 167:228-234
12. Spiekermann P, Rehm PHA, Kalscheuer R, Baumeister D, Steinbüchel A (1999) A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol 171:73-80
13. Vaneechoute M, Tjernberg I, Baldi F, Pepi M, Fani R, Sullivan ER, van der Toorn J, Dijkshoorn L (1999) Oil-degrading Acinetobacter strain RAG-1 and strains described as Acinetobacter venetianus sp. nov. belong to the same genomic species. Res Microbiol 150:69-73
14. Zhang J, Lee SH, Gross R, Kaplan D (1999) Surface properties of emulsan-analogs. J Chem Technol Biotechnol 74:759-765