메뉴 건너뛰기




Volumn 127, Issue 25, 2005, Pages 8912-8913

Using liquid crystals to report membrane proteins captured by affinity microcontact printing from cell lysates and membrane extracts

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR; GLYCOPROTEIN; MEMBRANE PROTEIN; MONOCLONAL ANTIBODY; PROTEIN TYROSINE KINASE;

EID: 21244502443     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja051079g     Document Type: Article
Times cited : (76)

References (19)
  • 11
    • 21244501841 scopus 로고    scopus 로고
    • note
    • The surface of the PDMS stamp was plasma oxidized (Plasma Etch PE-200, 8 sccm, 20 s, 100 W) and then immersed into an aqueous solution containing 10% 3-aminopropyltriethoxysilane at 80 °C for 1 h. A subsequent nucleophilic ring opening reaction between the amines on the surface of the stamp and 0.1 M succinic anhydride in N,N-dimethylformamide (room temperature, 10 min) produced a carboxylic acid-terminated surface. Anti-EGFR 111.6 (1 mg/mL) was then immobilized on the carboxylic acid-terminated surface using standard NHS-EDC protocols.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.