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Volumn 127, Issue 25, 2005, Pages 9085-9099

G-matrix Fourier transform NOESY-based protocol for high-quality protein structure determination

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL BONDS; FOURIER TRANSFORMS; MATRIX ALGEBRA; NUCLEAR MAGNETIC RESONANCE; PROTEINS; PROTONS;

EID: 21244479278     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0501870     Document Type: Article
Times cited : (58)

References (62)
  • 16
    • 21244505099 scopus 로고    scopus 로고
    • note
    • For the present study, an evaluation using the program CYANA was best suited. This is because we assigned for YqfB all intraresidue, sequential, and medium-range NOEs by predicting NOESY peak lists from chemical shift data and considering information on secondary structure elements (see Materials and Methods). Hence, a top-down algorithm appeared to be the natural choice to complement our chemical shift-based assignment protocol.
  • 41
    • 21244459654 scopus 로고    scopus 로고
    • note
    • 1H) relaxation.
  • 46
    • 21244472368 scopus 로고    scopus 로고
    • note
    • For example, the projected chemical shift evolution periods can be scaled once with κ = 1.0 and once with κ = 0.9. The latter results in a compression of the chemical shift multiplets by 10% in the second data set and enables one to unambiguously identify all components belonging to the same chemical shift multiplet by comparison of the two data sets (see Figure 5 in ref 12).
  • 49
    • 21244505872 scopus 로고    scopus 로고
    • Atreya, H. S.; Shen, Y.; Yee, A.; Arrowsmith, C. H.; Szyperski, T.
    • The structural biology will be published elsewhere (Atreya, H. S.; Shen, Y.; Yee, A.; Arrowsmith, C. H.; Szyperski, T.).
  • 57
    • 21244492479 scopus 로고    scopus 로고
    • note
    • l5N-resolved part.
  • 59
    • 21244442741 scopus 로고    scopus 로고
    • note
    • 3-CH3 NOEs (total: 29).
  • 61
    • 21244482203 scopus 로고    scopus 로고
    • note
    • aliphatic, the minimal measurement time is ∼6.5 h at 600 MHz (i.e., about half of the minimal measurement time of the aliphatic congener; Table 2).
  • 62
    • 21244457682 scopus 로고    scopus 로고
    • note
    • With such software, the precision and accuracy of the initial structure obtained with data set II (60 h measurement time; Table 4; Figures S2 and 8) can be expected be obtained with data set I (30 h measurement time).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.