Antibiotic susceptibilities of Legionella pneumophila strain Paris in THP-1 cells as determined by real-time PCR assay

Journal of Antimicrobial Chemotherapy

Volumn 55, Issue 6, 2005, Pages 866-871

  Roch, N ^a   Maurin, M ^a  

^a UNIV GRENOBLE ALPES   (France)

Author keywords

Antibacterials; Intracellular; Macrophages; Quantitative PCR

Indexed keywords

AMOXICILLIN; ANTIBIOTIC AGENT; CIPROFLOXACIN; DOXYCYCLINE; ERYTHROMYCIN; LEVOFLOXACIN; MOXIFLOXACIN; QUINOLINE DERIVED ANTIINFECTIVE AGENT; RIFAMPICIN;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM ISOLATE; COLONY FORMING UNIT; HUMAN; HUMAN CELL; IN VITRO STUDY; LEGIONELLA PNEUMOPHILA; MACROPHAGE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION;

ANTI-BACTERIAL AGENTS; CELL LINE; CULTURE MEDIA; HUMANS; LEGIONELLA PNEUMOPHILA; MACROPHAGES; MICROBIAL SENSITIVITY TESTS; POLYMERASE CHAIN REACTION;

EID: 21244477542     PISSN: 03057453     EISSN: None     Source Type: Journal    
DOI: 10.1093/jac/dki141     Document Type: Article

References (27)

1. Fields BS, Benson RF, Besser RE. Legionella and legionnaires' disease: 25 years of investigation. Clin Microbiol Rev 2002; 15: 506-26.
2. Doleans A, Aurell H, Reyrolle M et al. Clinical and environmental distributions of Legionella strains in France are different. J Clin Microbiol 2004; 42: 458-60.
3. Helbig JH, Bernander S, Castellani Pastoris MC et al. Pan-European study on culture-proven Legionnaires' disease: distribution of Legionella pneumophila serogroups and monoclonal subgroups. Eur J Clin Microbiol Infect Dis 2002; 21: 710-6.
4. Yu VL, Plouffe JF, Castellani Pastoris M et al. Distribution of Legionella species and serogroups isolated by culture in patients with sporadic community-acquired legionellosis: an international collaborative survey. J Infect Dis 2002; 186: 127-8.
5. Aurell H, Etienne J, Forey F. Legionella pneumophila serogroup 1 strain Paris: endemic distribution throughout France. J Clin Microbiol 2003; 41: 3320-2.
6. Lawrence C, Reyrolle M, Dubrou S et al. Single clonal origin of a high proportion of Legionella pneumophila serogroup 1 isolates from patients and the environment in the area of Paris, France, over a 10-year period. J Clin Microbiol 1999; 37: 2652-5.
7. Edelstein PH. Antimicrobial chemotherapy for Legionnaires' disease: time for a change. Ann Intern Med 1998; 129: 328-30.
8. Wellinghausen N, Frost C, Marre R. Detection of legionellae in hospital water samples by quantitative real-time Light Cycler PCR. Appl Environ Microbiol 2001; 67: 3985-93.
9. Fraser DW, Tsai TR, Orenstein W et al. Legionnaires' disease: description of an epidemic of pneumonia. N Engl J Med 1977; 297: 1189-97.
10. Howden BP, Stuart RL, Tallis G et al. Treatment and outcome of 104 hospitalized patients with legionnaires' disease. J Intern Med 2003; 33: 484-8.
11. Roig R, Rello J. Legionnaires' disease: a rational approach to therapy. J Antimicrob Chemother 2003; 51: 1119-29.
12. Yu VL, Greenberg RN, Zadeikis N et al. Levofloxacin efficacy in the treatment of community-acquired legionellosis. Chest 2004; 125: 2135-9.
13. Barker J, Scaife H, Brown MRW. Intraphagocytic growth induces an antibiotic-resistant phenotype of Legionella pneumophila. Antimicrob Agents Chemother 1995; 39: 2684-8.
14. Cirillo JD, Falkow S, Tompkins LS. Growth of Legionella pneumophila in Acanthamoeba castellani enhances invasion. Infect Immun 1994; 62: 3254-61.
15. Kunishima H, Takemura H, Yamamoto H et al. Evaluation of the activity of antimicrobial agents against Legionella pneumophila in human monocytic cell line, THP-1, and an alveolar epithelial cell line, A549. J Infect Chemother 2000; 6: 206-10.
16. Takemura H, Yamamoto H, Kunishima H et al. Evaluation of a human monocytic cell line THP-1 model for assay of the intracellular activities of antimicrobial agents against Legionella pneumophila. J Antimicrob Chemother 2000; 46: 589-94.
17. Baltch AL, Smith RP, Ritz W. Inhibitory and bactericidal activities of levofloxacin, ofloxacin, erythromycin, and rifampicin used singly and in combination against Legionella pneumophila. Antimicrob Agents Chemother 1995; 39: 1661-6.
18. Garcia MT, Pelaz C, Gimenez MJ et al. In vitro activities of gemifloxacin versus five quinolones and two macrolides against 271 Spanish isolates of Legionella pneumophila: influence of charcoal on susceptibility test results. Antimicrob Agents Chemother 2000; 44: 2176-8.
19. Jonas D, Engels I, Friedhoff C et al. Efficacy of moxifloxacin, trovafloxacin, clinafloxacin and levofloxacin against intracellular Legionella pneumophila. J Antimicrob Chemother 2001; 47: 147-52.
20. Schülin T, Wennerstein CB, Ferraro MJ et al. Susceptibilities of Legionella spp. to newer antimicrobials in vitro. Antimicrob Agents Chemother 1998; 42: 1520-3.
21. Higa F, Kusano N, Tateyama M et al. Simplified quantitative assay system for measuring activities of drugs against intracellular Legionella pneumophila. J Clin Microbiol 1998; 36: 1392-8.
22. Vilde JL, Dournon E, Rajagopalan P. Inhibition of Legionella pneumophila multiplication within human macrophages by antimicrobial agents. Antimicrob Agents Chemother 1986; 30: 743-8.
23. Nielsen K, Hindersson P, Hoiby N et al. Sequencing of the rpoB gene in Legionella pneumophila and characterization of mutations associated with rifampicin resistance in the Legionellaceae. Antimicrob Agents Chemother 2000; 44: 2679-83.
24. Jonas D, Engels I, Hartung D et al. Development and mechanism of fluoroquinolone resistance in Legionella pneumophila. J Antimicrob Chemother 2003; 51: 275-80.
25. Onody C, Matsiota-Bernard P, Nauciel C. Lack of resistance to erythromycin, rifampicin, and ciprofloxacin in 98 clinical isolates of Legionella pneumophila. J Antimicrob Chemother 1997; 39: 815-6.
26. Rolain JM, Stuhl L, Maurin M et al. Evaluation of antibiotic susceptibilities of three rickettsial species including Rickettsia felis by a quantitative PCR DNA assay. Antimicrob Agents Chemother 2002; 46: 2747-51.
27. Brennan RE, Samuel J. Evaluation of Coxiella burnetii antibiotic susceptibilities by real-time PCR assay. J Clin Microbiol 2003; 41: 1869-74.