Binding of Pseudomonas aeruginosa AlgZ to sites upstream of the algZ promoter leads to repression of transcription

Journal of Bacteriology

Volumn 187, Issue 13, 2005, Pages 4430-4443

  Ramsey, Deborah M ^a   Baynham, Patricia J ^a,b   Wozniak, Daniel J ^a  

^a WAKE FOREST SCHOOL OF MEDICINE   (United States)

^b ST EDWARD'S UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALGINIC ACID; BACTERIAL PROTEIN; BETA GALACTOSIDASE; DNA BINDING PROTEIN; HELIX LOOP HELIX PROTEIN; PROTEIN ALGZ; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; ARTICLE; BACTERIOPHAGE P22; BINDING AFFINITY; BINDING SITE; BIOSYNTHESIS; DNA BINDING; GEL MOBILITY SHIFT ASSAY; GENE CLUSTER; GENE CONTROL; GENE REPRESSION; GENETIC TRANSCRIPTION; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN FOOTPRINTING; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PSEUDOMONAS AERUGINOSA;

BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; CONSENSUS SEQUENCE; DNA-BINDING PROTEINS; MOLECULAR SEQUENCE DATA; OPERON; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PSEUDOMONAS AERUGINOSA; REPRESSOR PROTEINS; SPECIES SPECIFICITY; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION, GENETIC;

PSEUDOMONAS AERUGINOSA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM;

EID: 21144455097     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.187.13.4430-4443.2005     Document Type: Article

References (47)

1. Armstrong, D. S., K. Grimwood, J. B. Carlin, R. Carzino, J. P. Gutierrez, J. Hull, A. Olinsky, E. M. Phelan, C. F. Robertson, and P. D. Phelan. 1997. Lower airway inflammation in infants and young children with cystic fibrosis. Am. J. Respir. Crit. Care Med. 156:1197-1204.
2. Baynham, P. J., and D. J. Wozniak. 1996. Identification and characterization of AlgZ, an AlgT-dependent DNA-binding protein required for Pseudomonas aeruginosa algD transcription. Mol. Microbiol. 22:97-308.
3. Baynham, P. J., A. L. Brown, L. L. Hall, and D. J. Wozniak. 1999. Pseudomonas aeniginosa AlgZ, a ribbon-helix-heiix DNA-binding protein, is essential for alginate synthesis and algD transcriptional activation. Mol. Microbiol. 33:1069-1080.
4. Boucher, J. C., H. Yu, M. H. Mudd, and V. Deretic. 1997. Mucoid Pseudomonas aeruginosa in cystic fibrosis: characterization of muc mutations in clinical isolates and analysis of clearance in a mouse model of respiratory infection. Infect. Immun. 65:3838-3846.
5. Brown, N. L., J. V. Stoyanov, S. P. Kidd, and J. L. Hobman. 2003. The MerR family of transcriptional regulators. FEMS Microbiol. Rev. 27:145-163.
6. Burns, J. L., R. L. Gibson, S. McNamara, D. Yim, J. Emerson, M. Rosenfeld, P. Hiatt, K. McCoy, R. Castile, A. L. Smith, and B. W. Ramsey. 2001. Longitudinal assessment of Pseudomonas aeruginosa in young children with cystic fibrosis. J. Infect. Dis. 183:444-452.
7. Cabral, D. A., B. A. Loh, and D. P. Speert. 1987. Mucoid Pseudomonas aeruginosa resists nonopsonic phagocytosis by human neutrophils and macrophages. Pediatr. Res. 22:429-431.
8. Cystic Fibrosis Foundation. 2003. Cystic Fibrosis Foundation patient registry 2002 annual data report. Cystic Fibrosis Foundation, Bethesda, Md.
9. Darbre, A. 1986. Analytical methods, p. 294-295. In A. Darbre (ed.), Practical protein chemistry: a handbook. Wiley, New York, N.Y.
10. Drenkard, E., and F. M. Ausubel. 2002. Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation. Nature 416:740-743.
11. Evans, L. R., and A. Linker. 1973. Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa. J. Bacteriol. 116:915-924.
12. Fyfe, J. A. M., and J. R. W. Govan. 1980. Alginate synthesis in mucoid Pseudomonas aeruginosa: a chromosomal locus involved in control. J. Gen. Microbiol. 119:443-450.
13. Gu, D., Y. Zhou, V. Kallhoff, B. Baban, J. J. Tanner, and D. F. Becker. 2004. Identification and characterization of the DNA-binding domain of the multifunctional PutA flavoenzyme. J. Biol. Chem. 279:31171-31176.
14. Huffman, J. L., and R. G. Brennan. 2002. Prokaryotic transcription regulators: more than just the helix-turn-helix motif. Curr. Opin. Struct. Biol. 12: 98-106.
15. +] and pH but elevated viscosity. Proc. Natl. Acad. Sci. USA 98: 8119-8123.
16. Knight, K. L., and R. T. Sauer. 1989. Identification of functionally important residues in the DNA binding redon of the Mnt repressor. J. Biol. Chem. 264: 13706-13710.
17. Knight, K. L., and R. T. Sauer. 1992. Biochemical and genetic analysis of operator contacts made by residues within the β-sheet DNA binding motif of Mnt repressor. EMBO J. 11:215-223.
18. Lam, J., R. Chan, K. Lam, and J. W. Costerton. 1980. Production of mucoid microcolonies by Pseudomonas aeruginosa within infected lungs in cystic fibrosis. Infect. Immun. 28:546-556.
19. Linker, A. and R. S. Jones. 1966. A new polysaccharide resembling alginic acid isolated from Pseudomonads. J. Biol. Chem. 241:3845-3851.
20. Martin, D. W., M. J. Schurr, M. H. Mudd, J. R. Govan, B. W. Holloway, and V. Deretic. 1993. Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients. Proc. Natl. Acad. Sci. USA 90: 8377-8381.
21. Martin, D. W., M. J. Schurr, H. Yu, and V. Deretic. 1994. Analysis of promoters controlled by the putative sigma factor AlgU regulating conversion to mucoidy in Pseudomonas aeruginosa: relationship to stress response. J. Bacteriol. 176:6688-6696.
22. Mathee, K., C. J. McPherson, and D. E. Ohman. 1997. Posttranslational control of the algT (algU)-encoded sigma 22 for expression of the alginate regulon in Pseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN). J. Bacteriol. 179:3711-3720.
23. Matsui, H., B. R. Grubb, R. Tarran, S. H. Randell, J. T. Gatzy, C. W. Davis, and R. C. Boucher. 1998. Evidence for periciliary liquid layer depletion, not abnormal ion composition, in the pathogenesis of cystic fibrosis airways disease. Cell 95:1005-1015.
24. Mohr, C. D., D. W. Martin, W. M. Konyecsni, J. R. Govan, S. Lory, and V. Deretic. 1990. Role of the far-upstream sites of the algD promoter and the algR and rpoN genes in environmental modulation of mucoidy in Pseudomonas aeruginosa. J. Bacteriol. 172:6576-6580.
25. Oberer, M., K. Zangger, S. Prytulla, and W. Keller. 2002. The anti-toxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins. Biochem. J. 361:41-47.
26. Ohman, D. E., and A. M. Chakrabarty. 1981. Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in a Pseudomonas aeruginosa cystic fibrosis isolate. Infect. Immun. 33:142-148.
27. Pedersen, S. S., A. Kharazmi, F. Espersen, and N. Hoiby. 1990. Pseudomonas aeruginosa alginate in cystic fibrosis sputum and the inflammatory response. Infect. Immun. 58:3363-3368.
28. Pressler, T., E. T. Jensen, F. Espersen, S. S. Pedersen, and N. Hoiby. 1995. High levels of complement-activation capacity in sera from patients with cystic fibrosis correlate with high levels of IgG3 antibodies to Pseudomonas aeruginosa antigens and poor lung function. Pediatr. Pulmonol. 20:71-77.
29. Raumann, B. E., M. A. Rould, C. O. Pabo, and R. T. Sauer. 1994. DNA recognition by β-sheets in the Arc repressor-operator crystal structure. Nature 367:754-757.
30. Reddy, M. M., and P. M. Quinton. 2003. Control of dynamic CFTR selectivity by glutamate and ATP in epithelial cells. Nature 423:750-760.
31. Riordan, J. R., J. M. Rommens, B. Kerem, N. Alon, R. Rozmahel, Z. Grzelczak, J. Zielenski, S. Lok, N. Plavsic, J. L. Chou, et al. 1989. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245:1437.
32. Rosenfeld, M., R. L. Gibson, S. McNamara, J. Emerson, J. L. Burns, R. Castile, P. Hiatt, K. McCoy, C. B. Wilson, A. Inglis, A. Smith, T. R. Martin, and B. W. Ramsey. 2001. Early pulmonary infection, inflammation, and clinical outcomes in infants with cystic fibrosis. Pediatr. Pulmonol. 32:356-366.
33. Simpson, J. A., S. E. Smith, and R. T. Dean. 1988. Alginate inhibition of the uptake of Pseudomonas aeruginosa by macrophages. J. Gen. Microbiol. 134: 29-36.
34. Simpson, J. A., S. E. Smith, and R. T. Dean. 1989. Scavenging by alginate of free radicals released by macrophages. Free Radic. Biol. Med. 6:347-353.
35. Singh, P. K., A. L. Schaefer, M. R. Parsek, T. O. Moninger, M. J. Welsh, and E. P. Greenberg. 2000. Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms. Nature 407:762-764.
36. Smith, T. L., and R. T. Sauer. 1996. Dual regulation of open-complex formation and promoter clearance by Arc explains a novel repressor to activator switch. Proc. Natl. Acad. Sci. USA 93:8868-8872.
37. Snook, C. F., P. A. Tipton, and L. J. Beamer. 2003. Crystal structure of GDP-mannose dehydrogenase: a key enzyme of alginate biosynthesis in P. aeruginosa. Biochemistry 42:4658-4668.
38. Somers, W. S., and S. E. V. Phillips. 1992. Crystal structure of the Met repressor-operator complex at 2.8 Å resolution reveals DNA recognition by β-strands. Nature 359:387-393.
39. Stover, C. K., X. Q. Pham, A. L. Erwin, S. D. Mizoguchi, P. Warrener, M. J. Hickey, F. S. Brinkman, W. O. Hufnagle, D. J. Kowalik, M. Lagrou, R. L. Garber, L. Goltry, E. Tolentino, S. Westbrock-Wadman, Y. Yuan, L. L. Brody, S. N. Coulter, K. R. Folger, A. Kas, K. Larbig, R. Lim, K. Smith, D. Spencer, G. K. Wong, Z. Wu, I. T. Paulsen, J. Reizer, M. H. Saier, R. E. Hancock, S. Lory, and M. V. Olson. 2000. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406:959-964.
40. Tatnell, P. J., N. J. Russell, and P. Gacesa. 1993. A metabolic study of the activity of GDP-mannose dehydrogenase and concentrations of activated intermediates of alginate biosynthesis in Pseudomonas aeruginosa. J. Gen. Microbiol. 139:119-127.
41. Vershon, A. K., S. M. Liao, W. R. McClure, and R. T. Sauer. 1987. Bacteriophage P22 Mnt repressor. DNA binding and effects on transcription in vitro. J. Mol. Biol. 195:311-322.
42. Vershon, A. K., S. M. Liao, W. R. McClure, and R. T. Sauer. 1987. Interaction of the bacteriophage P22 Arc repressor with operator DNA. J. Mol. Biol. 195:323-331.
43. Vershon, A. K., P. Youderian, M. M. Susskind, and R. T. Sauer. 1985. The bacteriophage P22 Arc and Mnt repressors. J. Biol. Chem. 260:12124-12129.
44. Waldburger, C. D., and R. T. Sauer. 1995. Domains of Mnt repressor: roles in tetramer formation, protein stability, and operator DNA binding. Biochemistry 34:13109-13116.
45. Wozniak, D. J. 1994. Integration host factor and sequences downstream of the Pseudomonas aeruginosa algD transcription start site are required for expression. J. Bacteriol. 176:5068-5076.
46. Wozniak, D. J., and D. E. Ohman. 1994. Transcriptional analysis of the Pseudomonas aeruginosa genes algR, algB and algD reveals a hierarchy of alginate gene expression which is modulated by algT. J. Bacteriol. 176: 6007-0014.
47. Wozniak, D. J., A. B. Sprinkle, and P. J. Baynham. 2003. Control of Pseudomonas aeruginosa algZ expression by the alternative sigma factor AlgT. J. Bacteriol. 185:7297-7300.