Transgenic alfalfa secretes a fungal endochitinase protein to the rhizosphere

Plant and Soil

Volumn 269, Issue 1-2, 2005, Pages 233-243

  Tesfaye, Mesfin ^a   Denton, Matthew D ^a   Samac, Deborah A ^a   Vance, Carroll P ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Alfalfa; Endochitinase; Exudation; Lucerne; Medicago sativa; Recombinant protein; Signal peptide

Indexed keywords

ALFALFA; ENZYME ACTIVITY; FUNGAL DISEASE; RHIZOSPHERE; SPORE; TRANSGENIC PLANT;

CASSAVA VEIN MOSAIC VIRUS; MANIHOT ESCULENTA; MEDICAGO SATIVA;

EID: 21144444559     PISSN: 0032079X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11104-004-0520-0     Document Type: Conference Paper

References (28)

1. S Austin ET Bingham DE Mathews MN Shahan J. Will RR Burgess 1995 Production and field performance of transgenic alfalfa (Medicago sativa L.) expressing α-amylase and manganese-dependent lignin peroxidase Euphytica. 85 381 393
2. ET. Bingham 1991 Registration of alfalfa hybrid Regen-SY germplasm for tissue culture and transformation Crop Sci. 31 1098
3. JP Bolar JL Norelli KW Wong CK Hayes GE. Harman HS Aldwincke 2000 Expression of endochitinase from Trichoderma harzianum in transgenic apple increases resistance to apple scab and reduces vigor Phytopathology. 90 72 77
4. NV Borisjuk LG Broisjuk S Logendra F Petersen Y. Gleba I Raskin 1999 Production of recombinant proteins in plant root exudates Nature Biotechnol. 17 466 469
5. A Brants ED. Earle 2001 Trangenic tobacco cell cultures expressing Trichoderma harzianum endochitinase gene release the enzyme into the medium Plant Cell Rep. 20 73 78
6. U. Conrad U. Fiedler 1998 Compartment-specific accumulation of recombinant immunoglobulins in plant cells, an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity Plant Mol. Biol. 38 101 109
7. Cruz De La A Hidalgo-Gallego JM Lora T Benitez JA. Pintor-Toro A. Llobell 1992 Isolation and characterization of three chitinases from Trichoderma harzianum Eur. J. Biochem. 206 859 867
8. H Daniell SJ. Streatfield K Wycoff 2001 Medical molecular farming, production of antibodies, biopharmaceuticals and edible vaccines in plants Trends Plant Sci. 6 219 226
9. I Farran JJ Sanchez-Serrano JF Medina J. Prieto AM Mingo-Castel 2002 Targeted expression of human serum albumin to potato tubers Transgenic Res. 11 337 346
10. R Fischer D Schumann S Zimmermann J Drossard M. Sack S. Schillberg 1999 Expression and characterization of bispecific single-chain Fv fragments produced in transgenic plants Eur. J. Biochem. 262 810 816
11. S Haran S Logendra M Seskar M. Bratanova I Raskin 2000 Characterization of Arabidopsis acid phosphatase promoter and regulation of acid phosphatase expression Plant Physiol. 124 615 626
12. CK Hayes S Klemsdal M Lorito A Di Pietro C Peterbauer JP Nakas A. Tronsmo GE. Harman 1994 Isolation and sequence of an endochitinase-encoding gene from a cDNA library of Trichoderma harzianum Gene 138 143 148
13. AR Kusnadi ZL. Nikolov JA. Howard 1997 Production of recombinant proteins in transgenic plants, practical considerations Biotechnol. Bioeng. 56 473 484
14. UK Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685
15. M Lorito SL Woo IG Fernandez G Colucci GE Harman JA Pintor-Toro E Filippone S Muccifora CB Lawrence A Zoina S. Tuzun F. Scala 1998 Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogenens Proc. Natl. Acad. Sci. USA 95 7860 7865
16. Maniatis TA, Fritsch EF., Sambrook J. (1992). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
17. Marschner H and Römheld V 2001 Root-induced changes in the availability of micronutrients in the rhizosphere. In Plant Roots: The Hidden Half. Eds. Y Waise, A Eshel and U Kafkafi. pp. 557-581. Marcel Dekker, New York.
18. SS Miller J Liu DA Allan CJ Menzhuber M. Fedorova CP. Vance 2001 Molecular control of acid phosphatase secretion into the rhizosphere of proteoid roots from phosphorus-stressed white lupin Plant Physiol. 127 594 606
19. AA. Mora ED Earle 2001 Resistance to Alternaria brassicicola in transgenic broccoli expressing a Trichoderma harzianum endochitinase gene Mol. Breeding. 8 1 9
20. MJ Pozo C Azcon-Aguilar E. Dumas-Gaudot JM Barea 1998 Chitosanase and chitinase activities in tomato roots during interactions with arbuscular mycorrhizal fungi or Phytophthora parasitica J. Exp. Botany 49 1729 1739
21. Samac D A, Dornbusch M, Tesfaye Mesfin, Purev S and Temple S J 2004 A comparison of constitutive promoters for expression of transgenes in alfalfa Medicago sativa. Transgenic Res. (in press).
22. P Saruul F Srienc DA. Somers DA Samac 2002 Production of a biodegradable plastic polymer, poly-ß-hydroxybutyrate, in transgenic alfalfa Crop Sci. 42 919 927
23. E Stoger M Sack Y Perrin C Vaquero E Torres RM Twyman P. Christou R. Fischer 2002 Practical consideration for pharmaceutical antibody production in different crop systems Mol. Breeding 9 149 158
24. J. Trudel A Asselin 1989 Detection of chitinase activity after polyacrylamide gel electrophoresis Anal. Biochem. 178 362 366
25. Uren NC 2001 Types, amounts and possible functions of compounds released into the rhizosphere by soil-grown plants. In The Rhizosphere. Eds. R Pinton, Z Varanini and P Nannipieri. pp. 19-40. Marcel Dekker, New York.
26. B Verdaguer A de Kochko RN. Beachy C. Fauquet 1996 Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter Plant Mol. Biol. 31 1129 1139
27. TS Walker HP Bais E. Grotewold JM. Vivanco 2003 Root exudation and rhizosphere biology Plant Physiol. 132 44 51
28. Whipps JM.(1990) Carbon economy. In The Rhizosphere. Ed. J M Lynch. pp. 59-99. John Wiley and Sons, Chichester.