Determination of dioxins (PCDDs/PCDFs) and PCBs in food and feed using the DR CALUX® bioassay: Results of an international validation study

Food Additives and Contaminants

Volumn 22, Issue 5, 2005, Pages 472-481

  Gizzi, G ^a   Hoogenboom, L A P ^b   Von Holst, C ^a   Rose, M ^c   Anklam, E ^a  

^a JOINT RESEARCH CENTRE   (Italy)

^b WAGENINGEN UNIVERSITY   (Netherlands)

^c CENTRAL SCIENCE LABORATORY   (United Kingdom)

Author keywords

Bioassay; Dioxins; DR CALUX ; PCBs; Validation

Indexed keywords

DIOXIN; FISH OIL; LUCIFERASE; POLYCHLORINATED BIPHENYL DERIVATIVE; POLYCHLORINATED DIBENZODIOXIN; POLYCHLORINATED DIBENZOFURAN;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BIOASSAY; CHEMICAL ANALYSIS; CONTROLLED STUDY; FOOD ANALYSIS; FOOD CONTAMINATION; FOOD WASHING; GENE EXPRESSION; NONHUMAN; PERFORMANCE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; RAT; SAMPLING; STANDARDIZATION; TOXICITY TESTING; VALIDATION PROCESS;

ANIMAL FEED; ANIMALS; BENZOFURANS; BIOLOGICAL ASSAY; CELL LINE, TUMOR; DIOXINS; EUROPEAN UNION; FISH OILS; FOOD CONTAMINATION; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HERBICIDES; POLYCHLORINATED BIPHENYLS; RATS; REPRODUCIBILITY OF RESULTS; SOIL POLLUTANTS; TERATOGENS; TETRACHLORODIBENZODIOXIN;

EID: 21044453192     PISSN: 0265203X     EISSN: None     Source Type: Journal    
DOI: 10.1080/02652030500129196     Document Type: Article

References (23)

1. BDS. 2005. BioDetection System website http://www.bds.nl/
2. Behnisch PA, Hosoe K, Sakai S. 2003. Brominated dioxin-like compounds: In vitro assessment in comparison to classical dioxin-like compounds and other polyaromatic compounds. Environment International 29:861-877.
3. Berg van den M, Birnbaum LS, Bosveld ATC, Brunström B, Cook PH, Feeley M, Giesy JP, Hanberg A, Hasegawa R, Kennedy SW, Kubiak T, Larsen JC, Leeuwen FXR van, Liem AKD, Nolt C, Peterson RE, Poellinger L, Safe S, Schrenk D, Tillitt D, Tysklind M, Younes M, Wärn F, Zacharewski T. 1998. Toxic Equivalency Factors (TEFs) for PCBs, PCDDs, PCDFs for humans and wildlife. Environmental Health Perspectives 106:775-792.
4. Bovee TFH, Hoogenboom LAP, Hamers ARM, Aarts JMMJG, Brouwer A, Kuiper HA. 1998. Validation and use of the CALUX-bioassay for the detection of dioxins and coplanar PCBs in bovine milk. Food Additives and Contaminants 15:863-875.
5. Carbonnelle S, Loco J van, Overmeire I van, Windal I, Wouwe N van, Leeuwen S van, Goeyens L. 2004. Importance of REP values when comparing the CALUX bioassay results with chemoanalyses results; example with spiked vegetable oils. Talanta 63:1255-1259.
6. Denison MS, Zhao B, Baston DS, Clark GC, Murata H, Han D. 2004. Recombinant cell bioassay systems for the detection and relative quantitation of halogenated dioxins and related chemicals, Talanta 63:1123-1133.
7. EC. 2001. Council regulation (EC) No. 2375/2001 of 29 November 2001 amending Commission Regulation (EC) No 466/2001 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Communities L321:1-5.
8. EC. 2002a. Council directive 2001/102/EC of 27 November 2001 amending Directive a999/29/EC on the undesirable substances and products in animal nutrition. Official Journal of the European Communities L6:45-49.
9. EC. 2002b. Commission Recommendation 2002/201/EC of 4 March 2002 on the reduction of the presence of dioxins, furans and PCBs in feeding stuffs and foodstuffs. Official Journal of the European Communities L67:69-74.
10. EC. 2002c. Commission Directive 2002/69/EC of 26 July 2002 laying down the sampling methods and the methods of analysis for the official control of dioxins and the determination of dioxin-like PCBs in foodstuffs. Official Journal of the European Communities L209:5-14.
11. EC. 2002d. Commission Directive 2002/70/EC of 26 July 2002 establishing requirements for the determination of levels of dioxins and dioxin-like PCBs in feeding stuffs. Official Journal of the European Communities L209:15-21.
12. FAPAS. 2002. Environmental contaminants, FAPAS report No. 0612.
13. Hoogenboom LAP. 2001. Bioassays in contaminant analysis. In: Watson DH, editor. Food chemical safety, Vol. 1. Cambridge, UK: Woodhead Publishing Ltd. pp 91-106.
14. Hoogenboom LAP. 2002. The Combined use of the CALUX bioassay and the HRGC/HRMS method for the detection of novel dioxin sources and new dioxin-like compounds. Environmental Science and Pollution Research 9:9-11.
15. Hoogenboom LAP, Traag WA, Hoogerbrugge R, Baumann B, Pottier L, Weg G van der, Vries de J. 2004a. The use of the DR CALUX-bioassay and indicator-PCBs for screening of elevated levels of dioxins and dioxin-like PCBs in eel. Talanta 63:1249-1253.
16. Hoogenboom LAP, Traag WA, Hoogerbrugge R, Baumann B, Portier L, Weg G van der, Vries de J. 2004b. The use of the DR CALUX-bioassay and indicator-PCBs for screening of elevated levels of dioxins and dioxin-like PCBs in eel. Food Additives and Contaminants (in preparation).
17. Horwitz W. 1995. Protocol for the design, conduct and interpretation of method-performance studies. Pure and Applied Chemistry 67:331-343.
18. ISO. 2000. ISO 5725, Accuracy (trueness and precision) of measurement methods and results, parts 1 to 6. In: Statistical methods for quality control. Geneva: ISO Standards Handbook, International Standardization Organisation. (ISBN 92-67-10321-0).
19. Laier P, Cederberg T, Larsen JC, Vinggaard AM. 2003. Applicability of the CALUX bioassay for screening of dioxin levels in human milk samples. Food Additives and Contaminants 20:583-595.
20. Loco van J, Leeuwen SPJ van, Roos P, Carbonnelle S, Boer J de, Goeyens L, Beernaert H. 2004. The International validation of bio- and chemical-analytical screening methods for dioxins and dioxin-like PCBs: The DIFFERENCE project rounds 1 and 2. Talanta 63:1169-1182.
21. Overmeire I, van Struelens P, Windal I, Wouwe N, van Carbonelle S, Baeyens W, Goeyens L. 2004. Validation efforts for the analysis of animal fat using the CALUX method. Organohalogen Compounds 66:618-622.
22. Scippo M-L, Eppe G, de Pauw E, Maghuin-Rogister G. 2004. DR-CALUX® screening of food samples: Evaluation of the quantitative approach to measure dioxin, furans and dioxin-like PCBs. Talanta 63:1193-1202.
23. Schoeters G, Goyvaerts MP, Ooms DL, Cleuvenbergen R van. 2004. The evaluation of dioxin and dioxin-like contaminants in selected food samples obtained from the Belgian market: Comparison of TEQ measurements obtained through the CALUX bioassay with congener specific chemical analyses. Chemosphere 54:1289-1297.