Functional evaluation of bioartificial liver using RT-PCR

Bio-Medical Materials and Engineering

Volumn 15, Issue 3, 2005, Pages 211-218

  Fujita, Satoshi ^a   Ueda, Yuichiro ^a   Ko, In Kap ^a   Paek, Hyun Joon ^a   Sajiki, Toshinobu ^a   Ikai, Iwao ^b   Yamaoka, Yoshio ^b   Ikada, Yoshito ^c   Iwata, Hiroo ^a  

^a KYOTO UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c SUZUKA UNIVERSITY OF MEDICAL SCIENCE   (Japan)

Author keywords

Bioartificial liver; Chemical loading test; Function; Hepatocyte; RT PCR

Indexed keywords

BLOOD; DNA; ENZYMES; METABOLISM; PROTEINS; RNA;

BIOARTIFICIAL LIVER (BAL); CHEMICAL LOADING; PERFUSION CULTURE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR);

ARTIFICIAL ORGANS;

ALBUMIN; CYTOCHROME P450; LIDOCAINE; MESSENGER RNA;

ARTICLE; ARTIFICIAL LIVER; BIOARTIFICIAL ORGAN; BLOOD; CELL CULTURE; FUNCTIONAL ASSESSMENT; GENE AMPLIFICATION; GENE DELETION; GENE EXPRESSION; GENE TARGETING; HOLLOW FIBER; LIVER CELL; METABOLISM; NONHUMAN; PERFUSION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ALBUMINS; ANIMALS; ARYL HYDROCARBON HYDROXYLASES; BINDING, COMPETITIVE; BIOMEDICAL ENGINEERING; CYTOCHROME P-450 CYP3A; DNA; DNA PRIMERS; DNA, COMPLEMENTARY; GENE LIBRARY; HEPATOCYTES; HUMANS; LIVER; LIVER FAILURE, ACUTE; LIVER, ARTIFICIAL; OXIDOREDUCTASES, N-DEMETHYLATING; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SWINE; TIME FACTORS; TISSUE ENGINEERING;

SUIDAE;

EID: 21044439072     PISSN: 09592989     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. J.W. Allen, T. Hassanein and S.N. Bhatia, Advances in bioartificial liver devices, Hepatology 34 (2001), 447-455.
2. J. Chelly, J.C. Kaplan, P. Maire, S. Gautron and A. Kahn, Transcription of the dystrophin gene in human muscle and non-muscle tissue, Nature 333 (1988), 858-860.
3. W.M. Freeman, S.J. Walker and K.E. Vrana, Quantitative RT-PCR: pitfalls and potential, Biotechniques 26 (1999), 112-122, 124-125.
4. G. Gilliland, S. Perrin, K. Blanchard and H.F. Bunn, Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction, Proc. Natl. Acad. Sci. USA 87 (1990), 2725-2729.
5. H. Iwata, T. Sajiki, H. Maeda, Y.G. Park, B. Zhu, S. Satoh, T. Uesugi, I. Ikai, Y. Yamaoka and Y. Ikada, In vitro evaluation of metabolic functions of a bioartificial liver, ASAIO J. 45 (1999), 299-306.
6. G.V. Mazariegos, J.F. Patzer 2nd, R.C. Lopez, M. Giraldo, M.E. Devera, T.A. Grogan, Y. Zhu, M.L. Fulmer, B.P. Amiot and D.J. Kramer, First clinical use of a novel bioartificial liver support system (BLSS), Am. J. Transplant. 2 (2002), 260-266.
7. C. Morrison and F. Gannon, The impact of the PCR plateau phase on quantitative PCR, Biochim. Biophys. Acta 1219 (1994), 493-498.
8. L. Raeymaekers, Basic principles of quantitative PCR, Mol. Biotechnol. 15 (2000), 115-122.
9. T. Sajiki, H. Iwata, H.J. Paek, T. Tosha, S. Fujita, Y. Ueda, Y.G. Park, B. Zhu, S. Satoh, I. Ikai, Y. Yamaoka and Y. Ikada, Morphologic studies of hepatocytes entrapped in hollow fibers of a bioartificial liver, ASAIO J. 46 (2000), 49-55.
10. T. Sajiki, H. Iwata, H.J. Paek, T. Tosha, S. Fujita, Y. Ueda, Y.G. Park, B. Zhu, S. Satoh, I. Ikai, Y. Yamaoka and Y. Ikada, Transmission electron microscopic study of hepatocytes in bioartificial liver, Tissue Eng. 6 (2000), 627-640.
11. I.M. Sauer, K. Zeilinger, N. Obermayer, G. Pless, A. Grunwald, A. Pascher, T. Mieder, S. Roth, M. Goetz, D. Kardassis, A. Mas, P. Neuhaus and J.C. Gerlach, Primary human liver cells as source for modular extracorporeal liver support - a preliminary report, Int. J. Artif. Organs 25 (2002), 1001-1005.
12. R.A. Shatford, S.L. Nyberg, S.J. Meier, J.G. White, W.D. Payne, W.S. Hu and F.B. Cerra, Hepatocyte function in a hollow fiber bioreactor: a potential bioartificial liver, J. Surg. Res. 53 (1992), 549-557.
13. P. Soucek, R. Zuber, E. Anzenbacherova, P. Anzenbacher and F.P. Guengerich, Minipig cytochrome P450 3A, 2A and 2C enzymes have similar properties to human analogs, BMC Pharmacol. 1 (2001), 11.
14. A.J. Strain and J.M. Neuberger, A bioartificial liver - state of the art, Science 295 (2002), 1005-1009.
15. T. Suzuki, P.J. Higgins and D.R. Crawford, Control selection for RNA quantitation, Biotechniques 29 (2000), 332-337.
16. K. Uberla, C. Platzer, T. Diamantstein and T. Blankenstein, Generation of competitor DNA fragments for quantitative PCR, PCR Methods Appl. 1(1991), 136-139.
17. M.P. van de Kerkhove, E. Di Florio, V. Scuderi, A. Mancini, A. Belli, A. Bracco, M. Dauri, G. Tisone, G. Di Nicuolo, P. Amoroso, A. Spadari, G. Lombardi, R. Hoekstra, F. Calise and R.A. Chamuleau, Phase I clinical trial with the AMC-bioartificial liver, Int. J. Artif. Organs 25 (2002), 950-959.
18. A.M. Wang, M.V. Doyle and D.F. Mark, Quantitation of mRNA by the polymerase chain reaction, Proc. Natl. Acad. Sci. USA 86 (1989), 9717-9721.
19. F.D. Watanabe, C.J. Mullon, W.R. Hewitt, N. Arkadopoulos, E. Kahaku, S. Eguchi, T. Khalili, W Arnaout, C.R. Shackleton, J. Rozga, B. Solomon and A.A. Demetriou, Clinical experience with a bioartificial liver in the treatment of severe liver failure. A phase I clinical trial, Ann. Surg. 225 (1997), 484-491.
20. D.Y Wu, L. Ugozzoli, B.K. Pal, J. Qian and R.B. Wallace, The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction, DNA Cell Biol. 10 (1991), 233-238.