Cellular manipulation of human embryonic stem cells by TAT-PDX1 protein transduction

Molecular Therapy

Volumn 12, Issue 1, 2005, Pages 28-32

  Kwon, Young Do ^a   Oh, Sun Kyung ^a   Kim, Hee Sun ^a   Ku, Seung Yup ^a   Kim, Seok Hyun ^a   Choi, Young Min ^a   Moon, Shin Yong ^a  

^a Seoul National University   (South Korea)

Author keywords

Human embryonic stem cells; PDX1; Protein transduction

Indexed keywords

FLUORESCEIN ISOTHIOCYANATE; HYBRID PROTEIN; INSULIN; TRANSACTIVATOR PROTEIN; TRANSCRIPTION FACTOR PDX 1;

ARTICLE; CELL BASED GENE THERAPY; CELL DIFFERENTIATION; CELL MANIPULATION; CELL TYPE; DNA INTEGRATION; EMBRYO; EMBRYO DEVELOPMENT; EMBRYONIC STEM CELL; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; LIPID BILAYER; MEDICAL RESEARCH; PANCREAS; PROTEIN DOMAIN; PROTEIN ENGINEERING;

ANIMALS; CELL LINE; FLUORESCEIN-5-ISOTHIOCYANATE; GENE PRODUCTS, TAT; HOMEODOMAIN PROTEINS; HUMANS; PEPTIDE FRAGMENTS; RABBITS; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEM CELLS; TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC; TRANSDUCTION, GENETIC;

EID: 20844449780     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymthe.2005.03.010     Document Type: Article

References (28)

1. J.A. Thomson et al. Embryonic stem cell lines derived from human blastocysts Science 282 1998 1145-1147
2. B.E. Reubinoff M.F. Pera C.Y. Fong A. Trounson and A. Bongso Embryonic stem cell lines from human blastocysts: Somatic differentiation in vitro Nat. Biotechnol. 18 2000 399-404
3. S.K. Oh et al. Derivation and characterization of new human embryonic stem cell lines, SNUhES1, SNUhES2 and SNUhES3 Stem Cells 25 2005 211-219
4. M. Amit et al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture Dev. Biol. 227 2000 271-278
5. J. Itskovitz-Eldor et al. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers Mol. Med. 6 2000 88-95
6. R. Eiges et al. Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells Curr. Biol. 11 2001 514-518
7. M. Gropp et al. Stable genetic modification of human embryonic stem cells by lentiviral vectors Mol. Ther. 7 2003 281-287
8. Y. Ma A. Ramezani R. Lewis R.G. Hawley and J.A. Thomson High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors Stem Cells 21 2003 111-117
9. A. Frankel and C. Pabo Cellular uptake of the Tat protein from human immunodeficiency virus Cell 55 1988 1189-1193
10. M. Green and P. Loewenstein Autonomous functional domains of chemically synthesized human immunodeficiency virus Tat trans-activator protein Cell 55 1988 1179-1188
11. Kip1 induces cell migration Nat. Med. 4 1998 1449-1452
12. S.R. Schwarze A. Ho A. Vocero-Akbani and S.F. Dowdy In vivo protein transduction: Delivery of a biologically active protein into the mouse Science 285 1999 1569-1572
13. J.S. Wadia R.V. Stan and S.F. Dowdy Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis Nat. Med. 10 2004 310-315
14. N. Shibagaki and M.C. Udey Dendritic cells transduced with protein antigens induce cytotoxic lymphocytes and elicit antitumor immunity J. Immunol. 168 2002 2393-2401
15. A. Astriab-Fisher D. Sergueev M. Fisher B.R. Shaw and R.L. Juliano Conjugates of antisense oligonucleotides with the Tat and antennapedia cell-penetrating peptides: Effects on cellular uptake, binding to target sequences, and biologic actions Pharm. Res. 19 2002 744-754
16. J. Krosl et al. In vitro expansion of hematopoietic stem cells by recombinant TAT-HOXB4 protein Nat. Med. 9 2003 1428-1432
17. S. Asoh et al. Protection against ischemic brain injury by protein therapeutics Proc. Natl. Acad. Sci. USA 99 2002 17107-17112
18. G. Cao et al. In vivo delivery of a Bcl-xL fusion protein containing the TAT protein transduction domain protects against ischemic brain injury and neuronal apoptosis J. Neurosci. 22 2002 5423-5431
19. P.S. Kabouridis M. Hasan J. Newson D.W. Gilroy and T. Lawrence Inhibition of NF-kappa B activity by a membrane-transducing mutant of I kappa B alpha J. Immunol. 169 2002 2587-2593
20. L.C. Murtaugh and D.A. Melton Genes, signals, and lineages in pancreas development Annu. Rev. Cell Dev. Biol. 19 2003 71-89
21. A. Grapin-Botton A.R. Majithia and D.A. Melton Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes Genes Dev. 15 2001 444-454
22. J.P. Richard et al. Cell-penetrating peptides: A reevaluation of the mechanism of cellular uptake J. Biol. Chem. 278 2003 585-590
23. S. Fawell et al. Tat-mediated delivery of heterologous proteins into cells Proc. Natl. Acad. Sci. USA 91 1994 664-668
24. H. Noguchi H. Kaneto G.C. Weir and S. Bonner-Weir PDX-1 protein containing its own antennapedia-like protein transduction domain can transduce pancreatic duct and islet cells Diabetes 52 2003 1732-1737
25. D. Derossi et al. Cell internalization of the third helix of the Antennapedia homeodomain is receptor-independent J. Biol. Chem. 271 1996 18188-18193
26. S.J. Kim et al. Contribution of the PI3K/Akt/PKB signal pathway to maintenance of self-renewal in human embryonic stem cells FEBS Lett. 579 2005 534-540
27. H. Hui and R. Perfetti Pancreas duodenum homeobox-1 regulates pancreas development during embryogenesis and islet cell function in adulthood Eur. J. Endocrinol. 146 2002 129-141
28. P. Blyszczuk et al. Expression of Pax4 in embryonic stem cells promotes differentiation of nestin-positive progenitor and insulin-producing cells Proc. Natl. Acad. Sci. USA 100 2003 998-1003