Large scale expansion of rat's osteoblasts in rotating wall vessel (RWV) bioreactor

Chinese Journal of Biomedical Engineering

Volumn 24, Issue 2, 2005, Pages 134-139

  Song, Ke Dong ^a   Liu, Tian Qing ^a   Cui, Zhan Feng ^b   Li, Xiang Qin ^a   Ge, Dan ^a   Sun, Xiang Yu ^a   Ma, Xue Hu ^a  

^a DALIAN UNIVERSITY OF TECHNOLOGY   (China)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

In vitro; Microcarrier; Osteoblasts; Rotating wall vessel (RWV) bioreactor

Indexed keywords

CELL CULTURE; CELLS; HISTOLOGY; LIVING SYSTEMS STUDIES; SCANNING ELECTRON MICROSCOPY;

ALKALINE PHOSPHATASE (AP); BIOLOGICAL FUNCTIONS; MICROCARRIERS; ROTATING WALL VESSELS;

BIOREACTORS;

ALKALINE PHOSPHATASE;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL FUNCTION; IN VITRO STUDY; MICROCARRIER CULTURE; MORPHOMETRICS; NONHUMAN; OSTEOBLAST; PHASE CONTRAST MICROSCOPE; RAT; SCANNING ELECTRON MICROSCOPE; SUSPENSION CELL CULTURE;

ANIMALIA;

EID: 20444507206     PISSN: 02588021     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Lei Renl, Kanji Tsuru, Satoshi Hayakawa, Akiyoshi Osaka. Novel approach to fabricate porous gelatin-siloxane hybrids for bone tissue engineering. Biomaterials, 2002, 23(24):4765-4773.
2. Susan L, Ishaug-Riley, Genevieve M. Crane-Kruger, Michael. J Yaszemski, Antonios G, Mikos. Three-dimensional culture of rat calvarial osteoblasts in porous biodegradable polymers. Biomaterials, 2003, 19(15):1405-1412.
3. Langer R. Tissue engineering. Science, 1993, 260(5110):529.
4. Vacanti CA, Vacanti JP. Tissue-engineered morphojenesis of cartilage and bone by means of cells transplantation using synthetic biodegradable polymer matrices. J Clin Plast Surg, 1994, 21(3):445-451.
5. Van wezel AL. Growth of cell-strains and primary cells on microcarriers in homogeneous culture. Nature, 1967, 216:64.
6. Levien DW, et al. Cell Culture and its application. Academic Press, 1977, 191-216.
7. Freed LE, Vunjak NG. Microgravity tissue engineering. J In Vitro Cell Dev Biol-Animal, 1997;33:381-385.
8. Tan Wen-song, Liu Jian, Zhang Yuan-Xing. Application of the airlift bioreactor for hybridom a cell cultures. J Chinese Journal of Biotechnology, 1996, 12(4):477-481.
9. Kong D, Cardak S, Chen M, et al. High cell density and productivity culture of Chinese hamster ovary cells in a fluidized bed bioreactor. J Cytotechnology, 1999, 29:215-220.
10. Meissner P, Schroder B, Herfurth C, et al. Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells. J Cytotechnology, 1999, 30:227-234.
11. Schwarz RP, Goodwin TJ, Wolf DA. Cell culture for three-dimensional moldeling in rotating-wall vessels: an application of simulated microgravity. J Tissue Cult Method, 1992, 14:51-58.
12. Jessup JM, Goodwin TJ, Spaulding G. Prospects for use of microgravity-based bioreactors to study three-dimensional host-tumor interactions in human neoplasia. J Cell Biochem, 1993, 51:290-300.
13. Mosmann T. Rapid colorimetric assay for cellar growth and surival. Application to proliferation and cytotoxicity assays. J Immunol Methods., 1983, 65:55.
14. Hu WS. Large-scale mammalian cell culture. J Biotech and Bioeng, 1982, 24(2):365-369.