A novel assay for protein phosphatase 2A (PP2A) complexes in vivo reveals differential effects of covalent modifications on different Saccharomyces cerevisiae PP2A heterotrimers

Eukaryotic Cell

Volumn 4, Issue 6, 2005, Pages 1029-1040

  Gentry, Matthew S ^a,c   Li, Yikun ^b   Wei, Huijun ^b,d   Syed, Farhana F ^a   Patel, Sameer H ^b   Hallberg, Richard L ^a   Pallas, David C ^b  

^a SYRACUSE UNIVERSITY   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN SUBUNIT; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; BIOLOGICAL MODEL; CENTROMERE; CHEMISTRY; CYTOLOGY; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; METHYLATION; MUTATION; PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PROCESSING; SACCHAROMYCES CEREVISIAE;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, FUNGAL; GREEN FLUORESCENT PROTEINS; KINETOCHORES; METHYLATION; MODELS, BIOLOGICAL; MUTATION; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHORYLATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN SUBUNITS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 20444485337     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.4.6.1029-1040.2005     Document Type: Article

References (38)

1. Bryant, J. C., R. S. Westphal, and B. E. Wadzinski. 1999. Methylated C-terminal leucine residue of PP2A catalytic subunit is important for binding of regulatory Balpha subunit. Biochem. J. 339:241-246.
2. Chen, J., B. L. Martin, and D. L. Brautigan. 1992. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation. Science 257:1261-1264.
3. Chung, H., A. C. Nairn, K. Murata, and D. L. Brautigan. 1999. Mutation of Tyr307 and Leu309 in the protein phosphatase 2A catalytic subunit favors association with the alpha 4 subunit which promotes dephosphorylation of elongation factor-2. Biochemistry 38:10371-10376.
4. Di Como, C. J., and K. T. Arndt. 1996. Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases. Genes Dev. 10:1904-1916.
5. Evans, D. R., and B. A. Hemmings. 2000. Mutation of the C-terminal leucine residue of PP2Ac inhibits PR55/B subunit binding and confers supersensitivity to microtubule destabilization in Saccharomyces cerevisiae. Mol. Gen. Genet. 264:425-432.
6. Gentry, M. S., and R. L. Hallberg. 2002. Localization of Saccharomyces cerevisiae protein phosphatase 2A subunits throughout mitotic cell cycle. Mol. Biol. Cell 13:3477-3492.
7. Guo, H., and Z. Damuni. 1993. Autophosphorylation-activated protein kinase phosphorylates and inactivates protein phosphatase 2A. Proc. Natl. Acad. Sci. USA 90:2500-2504.
8. Healy, A. M., S. Zolnierowicz, A. E. Stapleton, M. Goebl, A. A. DePaoli-Roach, and J. R. Pringle. 1991. CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase. Mol. Cell. Biol. 11:5767-5780.
9. Janssens, V., and J. Goris. 2001. Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem. J. 353:417-439.
10. Janssens, V., J. Goris, and C. Van Hoof. 2005. PP2A: the expected tumor suppressor. Curr. Opin. Genet Dev. 15:34-41.
11. Kaiser, S., S. Michaelis, and A. Mitchell. 1994. Methods in yeast genetics, p. 234. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
12. Kalhor, H. R., K. Luk, A. Ramos, P. Zobel-Thropp, and S. Clarke. 2001. Protein phosphatase methyltransferase 1 (Ppm1p) is the sole activity responsible for modification of the major forms of protein phosphatase 2A in yeast. Arch. Biochem. Biophys. 395:239-245.
13. Kloeker, S., J. C. Bryant, S. Strack, R. J. Colbran, and B. E. Wadzinski. 1997. Carboxymethylation of nuclear protein serine/threonine phosphatase X. Biochem. J. 327:481-486.
14. Kremmer, E., K. Ohst, J. Kiefer, N. Brewis, and G. Walter. 1997. Separation of PP2A core enzyme and holoenzyme with monoclonal antibodies against the regulatory A subunit: abundant expression of both forms in cells. Mol. Cell. Biol. 7:1692-1701.
15. Li, X., A. Scuderi, A. Letsou, and D. M. Virshup. 2002. B56-associated protein phosphatase 2A is required for survival and protects from apoptosis in Drosophila melanogaster. Mol. Cell. Biol. 22:367-3684.
16. Lin, F. C., and K. T. Arndt. 1995. The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis. EMBO J. 14:2745-2759.
17. Millward, T. A., S. Zolnierowicz, and B. A. Hemmings. 1999. Regulation of protein kinase cascades by protein phosphatase 2A. Trends Biochem. Sci. 24:186-191.
18. Nickels, J. T., and J. R. Broach. 1996. A ceramide-activated protein phosphatase mediates ceramide-induced G1 arrest of Saccharomyces cerevisiae. Genes Dev. 10:382-394.
19. Ogris, E., D. M. Gibson, and D. C. Pallas. 1997. Protein phosphatase 2A subunit assembly: the catalytic subunit carboxy terminus is important for binding cellular B subunit but not polyomavirus middle tumor antigen. Oncogene 15:911-917.
20. Ogris, E., I. Mudrak, E. Mak, D. Gibson, and D. C. Pallas. 1999. Catalytically inactive protein phosphatase 2A can bind to polyomavirus middle tumor antigen and support complex formation with pp60(c-src). J. Virol. 73:7390-7398.
21. Pallas, D. C., L. K. Shahrik, B. L. Martin, S. Jaspers, T. B. Miller, D. L. Brautigan, and T. M. Roberts. 1990. Polyoma small and middle T antigens and SV40 small t antigen form stable complexes with protein phosphatase 2A. Cell 60:167-176.
22. Ronne, H., M. Carlberg, G. Z. Hu, and J. O. Nehlin. 1991. Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis. Mol. Cell. Biol. 11:4876-4884.
23. Roopchand, D. E., J. M. Lee, S. Shahinian, D. Paquette, H. Bussey, and P. E. Branton. 2001. Toxicity of human adenovirus E4orf4 protein in Saccharomyces cerevisiae results from interactions with the Cdc55 regulatory B subunit of PP2A. Oncogene 20:5279-5290.
24. Rose, M. D., R. Winston, and P. Heiter. 1990. Methods of yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. Shu, Y., and R. L. Hallberg. 1995. SCS1, a multicopy suppressor of hsp60-ts mutant alleles, does not encode a mitochondrially targeted protein. Mol. Cell. Biol. 15:5618-5626.
27. Shu, Y., H. Yang, E. Hallberg, and R. Hallberg. 1997. Molecular genetic analysis of Rts1p, a B′ regulatory subunit of Saccharomyces cerevisiae protein phosphatase 2A. Mol. Cell. Biol. 17:3242-3253.
28. Silverstein, A. M., C. A. Barrow, A. J. Davis, and M. C. Mumby. 2002. Actions of PP2A on the MAP kinase pathway and apoptosis are mediated by distinct regulatory subunits. Proc. Natl. Acad. Sci. USA 99:4221-4226.
29. Sneddon, A. A., P. T. Cohen, and M. J. Stark. 1990. Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes. EMBO J. 9:4339-4346.
30. Sontag, E., V. Nunbhakdi-Craig, G. Lee, R. Brandt, C. Kamibayashi, J. Kuret, C. L. White III, M. C. Mumby, and G. S. Bloom. 1999. Molecular interactions among protein phosphatase 2A, tau, and microtubules. Implications for the regulation of tau phosphorylation and the development of tauopathies. J. Biol. Chem. 274:25490-25498.
31. Tian, Q., and J. Wang. 2002. Role of serine/threonine protein phosphatase in Alzheimer's disease. Neurosignals 11:262-269.
32. Tolstykh, T., J. Lee, S. Vafai, and J. B. Stock. 2000. Carboxyl methylation regulates phosphoprotein phosphatase 2A by controlling the association of regulatory B subunits. EMBO J. 19:5682-5691.
33. van Zyl, W., W. Huang, A. A. Sneddon, M. Stark, S. Camier, M. Werner, C. Marck, A. Sentenac, and J. R. Broach. 1992. Inactivation of the protein phosphatase 2A regulatory subunit A results in morphological and transcriptional defects in Saccharomyces cerevisiae. Mol. Cell. Biol. 12:4946-4959.
34. van Zyl, W. H., N. Wills, and J. R. Broach. 1989. A general screen for mutant of Saccharomyces cerevisiae deficient in tRNA biosynthesis. Genetics 123:55-68.
35. Virshup, D. M. 2000. Protein phosphatase 2A: a panoply of enzymes. Curr. Opin. Cell Biol. 12:180-185.
36. Wei, H., D. G. Ashby, C. S. Moreno, E. Ogris, F. M. Yeong, A. H. Corbett, and D. C. Pallas. 2001. Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p. J. Biol. Chem. 276:1570-1577.
37. Wu, J., T. Tolstykh, J. Lee, K. Boyd, J. B. Stock, and J. R. Broach. 2000. Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo. EMBO J. 19:5672-5681.
38. Yu, X. X., X. Du, C. S. Moreno, R. E. Green, E. Ogris, Q. Feng, L. Chou, M. J. McQuoid, and D. C. Pallas. 2001. Methylation of the protein phosphatase 2A catalytic subunit is essential for association of Balpha regulatory subunit but not SG2NA, striatin, or polyomavirus middle tumor antigen. Mol. Biol. Cell 12:185-199.