Expression and purification of human endostatin from Hansenula polymorpha A16

Protein Expression and Purification

Volumn 42, Issue 1, 2005, Pages 12-19

  Wu, Jiangxue ^a,b   Fu, Wuzhao ^a   Luo, Jinxian ^a   Zhang, Tianyuan ^a  

^a SUN YAT SEN UNIVERSITY   (China)

^b SUN YAT SEN UNIVERSITY CANCER CENTER   (China)

Author keywords

Angiogenesis; Endothelial cell proliferation; Gene expression; Hansenula polymorpha; Human endostatin; Purification

Indexed keywords

ENDOSTATIN; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ANGIOGENESIS; ANIMAL; ARTICLE; BIOSYNTHESIS; C57BL MOUSE; CELL LINE; CELL PROLIFERATION; CHICK EMBRYO; CHORIOALLANTOIS; CYTOLOGY; DRUG EFFECT; ENDOTHELIUM CELL; GENE EXPRESSION; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOUSE; PICHIA; TUMOR CELL LINE; UMBILICAL VEIN; VASCULARIZATION;

ANIMALS; CELL LINE; CELL LINE, TUMOR; CELL PROLIFERATION; CHICK EMBRYO; CHORIOALLANTOIC MEMBRANE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; ENDOSTATINS; ENDOTHELIAL CELLS; GENE EXPRESSION; GENETIC VECTORS; HUMANS; MICE; MICE, INBRED C57BL; NEOVASCULARIZATION, PHYSIOLOGIC; PICHIA; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC; UMBILICAL VEINS;

GALLUS GALLUS; MURINAE; PICHIA ANGUSTA;

EID: 20444402318     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.09.022     Document Type: Article

References (14)

1. M.S. O'Reilly, T. Boehm, Y. Shing, N. Fukai, G. Vasios, W.S. Lane, E. Flynn, J.R. Birkhead, B.R. Olsen, and J. Folkman Endostatin: an endogenous inhibitor of angiogenesis and tumor growth Cell 88 2 1997 277 285
2. M. Dhanabal, R. Ramchandran, R. Volk, I.E. Stillman, M. Lombardo, M.L. Iruela-Arispe, M. Simons, and V.P. Sukhatme Endostatin: yeast production, mutants, and antitumor effect in renal cell carcinoma Cancer Res. 59 1 1999 189 197
3. B.K. Sim Angiostatin and endostatin: endothelial cell-specific endogenous inhibitors of angiogenesis and tumor growth Angiogenesis 2 1 1998 37 48
4. T. Boehm, J. Folkman, T. Browder, and M.S. O'Reilly Antiangiogenic therapy of experimental cancer does not induce acquired drug resistance Nature 390 6658 1997 404 407
5. K.N. Faber, W. Harder, G. Ab, and M. Veenhuis Review: methylotrophic yeasts as factories for the production of foreign proteins Yeast 11 14 1995 1331 1344
6. K.N. Faber, S. Westra, H.R. Waterham, I. Keizer-Gunnink, W. Harder, and G.A. Veenhuis Foreign gene expression in Hansenula polymorpha. A system for the synthesis of small functional peptides Appl. Microbiol. Biotechnol. 45 1-2 1996 72 79
7. G. Gellissen Heterologous protein production in methylotrophic yeasts Appl. Microbiol. Biotechnol. 54 6 2000 741 750
8. T. Boehm, S. Pirie-Shepherd, L.B. Trinh, J. Shiloach, and J. Folkman Disruption of the KEX1 gene in Pichia pastoris allows expression of full-length murine and human endostatin Yeast 15 7 1999 563 572
9. Y.Y. Xu, T.Y. Zhang, and J.X. Luo Construction of integrative expression vector and expression of human angiostatin in Hansenlula polymorpha Acta Microbiol. Sin. 42 7 2002 582 586
10. J.X. Wu, W.Z. Fu, T.Y. Zhang, J.X. Luo, and Q.Y. Wu Expression of human endostatin in P. pastoris and the bioactivity of its product Acta Sci. Nat. Universitatis SunYatSeni 42 6 2003 83 86
11. J.X. Wu, W.Z. Fu, T.Y. Zhang, J.X. Luo, and Q.Y. Wu High cell density culture of the P. pastoris engineered strain habouring endostatin gene and the purification and bioactivity analysis of the expressed product Chin. J. Biochem. Mol. Biol. 20 2 2004 224 228
12. M.P. Verbeet, H. van Heerikhuizen, J. Klootwijk, R.D. Fontijn, and R.J. Planta Evolution of yeast ribosomal DNA: molecular cloning of the rDNA units of Kluyveromyces lactis and Hansenula wingei and their comparison with the rDNA units of other Saccharomycetoideae Mol. Gen. Genet. 195 1-2 1984 116 125
13. J. Sambrook, and D.W. Russell Molecular cloning. A laboratory manual third ed. 2001 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
14. K. Sreekrishna, R.G. Brankamp, K.E. Kropp, D.T. Blankenship, J.T. Tsay, P.L. Smith, J.D. Wierschke, A. Subramaniam, and L.A. Birkenberger Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris Gene 190 1 1997 55 62