Expression and purification of a functionally active recombinant GDP-mannosyltransferase (PimA) from Mycobacterium tuberculosis H37Rv

Protein Expression and Purification

Volumn 42, Issue 1, 2005, Pages 47-53

  Gu, Xiaoling ^a   Chen, Mao ^b   Wang, Qingzhong ^a   Zhang, Min ^a   Wang, Baolin ^a   Wang, Honghai ^a  

^a FUDAN UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY   (China)

Author keywords

Fusion expression; GDP mannosyltransferase; Mycobacterium tuberculosis; PimA

Indexed keywords

BACTERIAL PROTEIN; DIVALENT CATION; GDP MANNOSYLTRANSFERASE; GDP-MANNOSYLTRANSFERASE; GUANOSINE DIPHOSPHATE MANNOSE; MANNOSYLTRANSFERASE; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; CIRCULAR DICHROISM; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MYCOBACTERIUM TUBERCULOSIS;

BACTERIAL PROTEINS; CATIONS, DIVALENT; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENE EXPRESSION; GUANOSINE DIPHOSPHATE MANNOSE; KINETICS; MANNOSYLTRANSFERASES; MYCOBACTERIUM TUBERCULOSIS; RECOMBINANT PROTEINS;

CORYNEBACTERINEAE; ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 20444363161     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.03.015     Document Type: Article

References (23)

1. World Health Organization, Tuberculosis, Fact Sheet no 104, World Health Organization, Geneva. Available from: 〈http://www.who.int/mediacentre/ factsheets/who104/en/〉
2. J.D. Mckinney In vivo veritas: the search for TB drug targets goes live Nat. Med. 6 2000 1330 1333
3. Y. van Kooyk, and T.B.H. Geijtenbeek DC-SIGN: escape mechanism for pathogenesis Nat. Immunol. 3 2003 697 709
4. A. Vercellone, J. Nigou, and G. Puzo Relationships between the structure and the roles of lipoarabinomannans and related glycoconjugates in tuberculosis pathogenesis Front. Biosci. 3 1998 e149 e163
5. A. Venisse, J.M. Berjeaud, P. Chaurand, M. Gilleron, and G. Puzo Structural features of lipoarabinomannan from Mycobacterium bovis BCG. Determination of molecular mass by laser desorption mass spectrometry J. Biol. Chem. 268 1993 12401 12411
6. D. Chatterjee, and K.H. Khoo Mycobacterial lipoarabinomannan: an extraordinary lipoheteroglygan with profound physiological effects Glycobiology 8 1998 113 120
7. G.R. Strohmeier, and M.J. Fenton Roles of lipoarabinomannan in the pathogenesis Microb. Infect. 1 1999 709 717
8. J. Nigou, M. Gilleron, M. Rojas, L.F. Garcia, M. Thurnher, and G. Puzo Mycobacterial lipoarabinomannans: modulators of dendritic cell function and the apoptotic response Microb. Infect. 4 2002 945 953
9. J. Nigou, M. Gilleron, and G. Puzo Lipoarabinomannans: from structure to biosynthesis Biochimie 85 2003 153 166
10. G.S. Besra, C.B. Morehouse, and C.M. Rittner Biosynthesis of mycobacterial lipoarabinomannan Biochem. J. 272 1997 18460 18466
11. J. Kordulakova, M. Gilleron, K. Mikusova, G. Puzo, P.J. Brennan, B. Gicquel, and M. Jackson Definition of the first mannosylation step in phosphatidylinositol mannoside synthesis Biochem. J. 277 2002 31335 31344
12. M.L. Schaeffer, K.H. Khoo, G.S. Besra, D. Chatterjee, P.J. Brennan, J.T. Belisle, and J.M. Inammine The pimB gene of M. tuberculosis encodes a mannosyltransferase involved in lipoarabinomannan biosynthesis Biochem. J. 274 1999 31625 31631
13. P. Brennan, and C.E. Ballou Biosynthesis of mannophosphoinositides by Mycobacterium phlei. The family of dimannophosphoinositides Biochem. J. 242 1967 3046 3056
14. P. Brennan, and C.E. Ballou Biosynthesis of mannophosphoinositides by Mycobacterium phlei. Enzymatic acylation of the dimannophosphoinositides Biochem. J. 243 1968 2975 2984
15. S.T. cole, R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S.V. Gordon, K. Eiglmeier, S. Gas, C.E. Barry III, F. Tekaia, K. Badcock, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroys, T. Hornsby, K. Jagels, A. Krogh, J. Mclean, S. Moule, L. Murphy, K. Oliver, J. Osborne, M.A. Quail, M.A. Rajandream, J. Rogers, S. Rutter, K. Seeger, J. Skelton, R. Squares, J.E. Sulston, K. Taylor, S. Whitehead, and B.G. Barrel Deciphering the biology of M. tuberculosis from the complete genome sequence Nature 393 1998 319 326
16. P.L. Abdian, A.C. Lellouch, C. Gautier, L. Ielpi, and R.A. Geremia Identification of essential amino acids in the bacterial mannosyltransferase AceA Biochem. J. 275 2000 40568 40575
17. J.R.C. Muniz, C.A. Alaves, C. de Pieri, L.M. Beltramini, H.S. Selistre-de-Araujo, A.L. Vettore, F.R. da Silva, P. Arruda, R.C. Garratt, G. Oliva, and D.H.F. Souza Overexpression, purification, biochemical characterization and molecular modeling of recombinant GDP-mannosyltransferase (GumH) from Xylella fastidiosa Biochem. Biophys. Res. Commun. 315 2004 485 492
18. D. Higgins, J. Thompson, T. Gibson, J.D. Thompson, D.G. Higgins, and T.J. Gibson CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting position-specific gap penalties and weight matrix choice Nucleic Acids Res. 22 1994 4673 4680
19. M.M. Braford, R.A. McRorie, and W.L. Williams A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
20. J.T. Yang, C.S. Wu, and H.M. Martinez Calculation of protein conformation from circular dichroism Methods Enzymol. 130 1986 208 269
21. S. Gosselin, M. Alhussaini, M.B. Streiff, K. Takabayashi, and M.M. Palcic A continuous spectrophotometric assay for glycosyltransferase Anal. Biochem. 220 1994 92 97
22. A. Villaverde, and M.M. Carrio Protein aggregation in recombinant bacteria: biological role of inclusion bodies Biotechnol. Lett. 25 2003 1358 1395
23. P.H. Hirel, M.J. Schmitter, P. Dessen, G. Fayat, and S. Blanquet Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid Proc. Natl. Acad. Sci. USA 86 1989 8247 8251