Pilot-scale adenovirus seed production through concurrent virus release and concentration by hollow fiber filtration

Biotechnology Progress

Volumn 21, Issue 3, 2005, Pages 851-859

  Subramanian, Shyamsundar ^a   Altaras, Gina M ^a   Chen, Jennifer ^a   Hughes, Benjamin S ^a   Zhou, Weichang ^a,b   Altaras, Nedim Emil ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b PROTEIN DESIGN LABS INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CLARIFICATION; CONCENTRATION (PROCESS); GAIN CONTROL; MICROFILTRATION; ULTRAFILTRATION;

CELL LYSIS; FIBER FILTERS; PILOT-SCALE ADENOVIRUS; VIRUS SEEDS;

VIRUSES;

ADENOVIRUS; ARTICLE; ARTIFICIAL MEMBRANE; BIOREACTOR; CELL LINE; EQUIPMENT; EQUIPMENT DESIGN; EVALUATION; GROWTH, DEVELOPMENT AND AGING; HUMAN; INSTRUMENTATION; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; PILOT STUDY; POROSITY; PRENATAL DEVELOPMENT; RETINA; ULTRAFILTRATION; VIROLOGY; VIRUS CULTURE;

ADENOVIRIDAE; BIOREACTORS; CELL LINE; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; HUMANS; MEMBRANES, ARTIFICIAL; PILOT PROJECTS; POROSITY; RETINA; ULTRAFILTRATION; VIRUS CULTIVATION;

ADENOVIRIDAE; MAMMALIA;

EID: 20144365369     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp049561a     Document Type: Article

References (18)

1. Brose, D.; Dosmar, M.; Cates, S.; Hutchison F. Studies on the scale-up of cross-flow filtration devices. PDA J. Pharm. Sci. Technol. 1995, 50, 252-260.
2. Condon, R. G. G.; Connelly, N. V.; Frei, A.; Glowacki, E.; Yabannavar, V.; Batandolo, S. Methods for cultivating cells and propagating viruses. U.S. Patent 6,146,891, Nov. 14, 2000.
3. Connelly, N.; Condon, R.; Frei, A.; Batandolo, S.; Glowacki, E.; Yabannavar, V. A cell culture process for large-scale production of an adenoviral vector. Viral Vectors and Vaccines 5th Annual Meeting. Williamsburg, VA, Nov 16-19, 1998.
4. Dornonin, K.; Toth, K.; Kuppuswamy, M.; Krajcsi, P.; Tollefson, A. E.; Wold, W. S. M. Overexpression of the ADP (E3-11.6K) protein increases cell lysis and spread of adenovirus. Virology 305, 378-387.
5. Emini, E. 2002. A potential HIV-1 vaccine using a replication-defective adenoviral vaccine vector. 9th Conference on Retroviruses and Opportunistic Infections, Seattle, WA www.retroconference.org.
6. Fallaux, F. J.; Bout, A.; Van Der Velde, I.; Van Den Wollenberg, D. J. M.; Hehir, K. M.; Keegan, J. et al. New helper cells and matched early reagion 1-deleted adenovirus vectros prevent generation of replication-competent adenoviruses. Hum. Gene Therapy 1998, 9, 1909-1917.
7. Nyberg-Hoffman, C.; Shabram, P.; Li, W.; Giroux, D.; Aguilar-Cordova, E. Sensitivity and reproducibility in adenoviral infectious titer determination. Nat. Med. 1997, 3, 808-811.
8. Maiorella, B.; Dorin, D.; Carion, A.; Harano, D. Cross-flow microfiltration of animal cells. Biotechnol. Bioeng. 1991, 37, 121-126.
9. Mittereder, N.; March, K. L.; Trapnell, B. Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy. J. Virol. 1996, 70, 7498-7509.
10. Russotti, G.;, Goklen, K. Cross-flow membrane filtration of fermentation broth. In Membrane Separations in Biotechnology, 2nd ed.; Wang, William, Ed.; Marcel Dekker: New York, 2000.
11. Schoofs, G.; Monica, T. J. et al. A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy. Cytotecknology 1998, 28 , 81-89.
12. Shabram, P. W.; Giroux, D. D.; Goudreau, A. M.; Gregory, R. J.; Horn, M. T.; Huyghe, B. G.; Liu, X.; Hunnally, M. H.; Sugarmna, B. J.; Sutjipto, S. Analytical anion-exchange HPLC of recombinant type-5 adenoviral particles. Hum. Gene Ther. 1997, 8, 453-465.
13. Shiver, J. W.; Fu, T. M.; Chen, L.; Casimiro, D. R.; Davies, M. E. et al. Replication-incompetent adenoviral vaccine elicits effective anti-immunodeficiency-virus immunity. Nature 2002, 415, 331-335.
14. Shiver, J. W.; Emini, E. A. Recent advances in the development of HIV-1 vaccines using replication-incompetent adenovirus vectors. Annu. Rev. Med. 2004, 55, 355-372.
15. van Reis, R.; Goodrich, E. M.; Yson, C. L.; Frautschy, L. N.; Dzengelski, S.; Lutz, H. Linear scale ultrafiltration. Biotechnol. Bioeng. 1997, 55, 737-746.
16. van Reis, R.; Leonard, L. C.; Hsu, C. C.; Builder, S. E. Industrial scale harvest of proteins from mammalian cell culture by tangential flow filtration. Biotechnol. Bioeng. 1991, 38, 413-422.
17. Wang, F.; Puddy, A. C.; Mathis, W. C.; Hager, M. A. G.; Louis, A. A.; McMackin, J. L.; Xu, J.; Zhang, Y.; Tan, C. Y.; Schofield, T. L.; Wolf, J. J.; Lewis, J. A. Using QPCR to assign infectious potencies to adenovirus based vaccines and vectors for gene therapy. Toward a universal method for the facile quantitation of virus and vector potency. Vaccine, submitted for publication.
18. Xie, L.; Metallo, C.; Warren, J.; Pilbrough, W.; Peltier, J.; Zhong, T.; Pikus, L.; Yancy, A.; Leung, J.; Aunins, J. G.; Zhou, W. Large-scale propagation of a replication-defective adenovirus vector in stirred-tank bioreactor PER. C6™ cell culture under sparging conditions. Biotechnol. Bioeng. 2003, 83, 45-52.