Secretory production of Rahnella aquatilis ATCC 33071 levansucrase expressed in Escherichia coli

Journal of Microbiology and Biotechnology

Volumn 14, Issue 6, 2004, Pages 1232-1238

  Kang, Soon Ah ^a   Lee, Jae Cheol ^b   Park, Young Min ^c   Lee, Chan ^d   Kim, Seung Hwan ^e   Chang, Byung Il ^f   Kim, Chul Ho ^g   Seo, Jeong Woo ^g   Rhee, Sang Ki ^g   Jung, Sung Je ^h   Kim, Sang Moo ⁱ   Park, Seong Kyu ^j   Jang, Ki Hyo ^b  

^a Konkuk University   (South Korea)

^b Samcheok National University   (South Korea)

^c Yeojoo Institute of Technology   (South Korea)

^d Hanseo University   (South Korea)

^e Korea Food and Drug Administration   (South Korea)

^f RealBiotech Co Ltd   (South Korea)

^g Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^h Kyung Hee University   (South Korea)

ⁱ Gangneung Wonju National University   (South Korea)

^j Kyung Hee University   (South Korea)

more..

Author keywords

Functional food; Levan; Levansucrase; Rahnella aquatilis; Secretion

Indexed keywords

GLYCINE; LEVAN; LEVANSUCRASE; RECOMBINANT ENZYME; TOLUENE;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM; ENZYME ACTIVITY; ENZYME RELEASE; ESCHERICHIA COLI; GENE EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INCUBATION TIME; MOLECULAR CLONING; MOLECULAR SIZE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERIZATION; PROTEIN EXPRESSION; RAHNELLA AQUATILIS; TEMPERATURE; THERMOSTABILITY;

ESCHERICHIA COLI; RAHNELLA AQUATILIS;

EID: 19944427275     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Blight, M. A., C. Chervaux, and I. B. Holland. 1994. Protein secretion pathways in Escherichia coli. Curr. Opin. Biotech. 5: 468-474
2. Bradford, M. M. 1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein of protein-dye binding. Anal. Biochem. 72: 248-254.
3. Calazans, G. M. T., R. C. Lima, F. P. Francisca, and C. E. Lopes. 2000. Molecular weight and antitumor activity of Zymomonas mobilis. Int. J. Biol. Macromol. 27: 245-247.
4. Chalmers, J. J., E. Kim, J. N. Telford, E. Y. Wong, W. C. Tacon, M. L. Shuler, and D. B. Wilson. 1990. Effects of temperature on Escherichia coli overproducing β-lactamase or human epidermal growth factor. Appl. Environ. Microbiol. 56: 104-111.
5. Chun U. H. and P. L. Rogers. 1988. The simultaneous production of sorbitol from fructose and gluconic acid from glucose using an oxidoreductase of Zymomonas mobilis. App. Microbiol. Biotechnol. 29: 19-24.
6. Goebel, W. and J. Hedgpeth. 1982. Cloning and functional characterization of the plasmid-encoded hemolysin determinant of Escherichia coli. J. Bacteriol. 151: 290-298.
7. Hammes, W., K. H. Scheifer, and O. Kandler. 1973. Mode of action of glycine on the biosynthesis of peptidoglycan. J. Bacteriol. 116: 1029-1053.
8. Han Y. W. 1990. Microbial levan. Adv. Appl. Microbiol. 35: 171-194.
9. Hendry, G. A. F. and R. K. Wallace. 1993. The origin, distribution, and evolutionary significance of fructans. In M. Suzuki and N. J. Chatterton (eds.), Science and Technology of Fructans. CRC Press.
10. Ikura, Y. 1986. Effect of glycine and its derivatives on production and release of beta-galactosidase by Escherichia coli. Agric. Biol. Chem. 50: 2747-2753.
11. Jang, E. K., K. H. Jang, I. Koh, I. H. Kim, S. H. Kim, S. A. Kang, C. H. Kim, S. D. Ha, and S. K. Rhee. 2002. Molecular characterization of the levansucrase gene from Pseudomonas aurantiaca S-4380 and its expression in Escherchia coli. J. Microbiol. Biotechnol. 12: 603-609.
12. Jang, K. H., J. W. Seo, K. B. Song, C. H. Kim, and S. K. Rhee. 1999. Extracellular secretion of levansucrase from Zymomonas mobilis in Escherichia coli. Bioprocess Eng. 21: 453-458.
13. Jang, K. H., K. B. Song, C. H. Kim, B. H. Chung, S. A. Kang, U. H. Chun, R. W. Choue, and S. K. Rhee. 2001. Comparison of characteristics of levan produced by different preparations of levansucrase from Zymomonas mobilis. Biotechnol. Lett. 23: 339-344.
14. John E. Van W. and A. D. Johannis. 1990. How big is the periplasmic space? Trends Biochem. Sci. 15: 136-137.
15. Kim, H. J., H. E. Park, M. J. Kim, H. G. Lee, J. Y. Yang, and J. H. Cha. 2003. Enzymatic characterization of a recombinant levansucrase from Rahnella aquatilis ATCC 15552. J. Microbiol. Biotechnol. 13: 230-235.
16. Kim, S. H., E. K. Jang, I. H. Kim, K. H. Jang, S. A. Kang, and B. I. Chang. 2003. Effect of glycine supplement on extracellular secretion of levansucrase from Pseudomonas aurantiaca S-4380 in recombinant Escherichia coli. Korean J. Biotechnol. Bioeng. 18: 312-317.
17. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
18. Leibovici J., S. Kopel, A. Siegel, and O. Gal-Mor. 1986. Effect of tumor inhibitory and stimulatory doses of levan, alone and in combination with cyclophosphamide, on spleen and lymph nodes. Int. J. Immunopharmacol. 8: 391-403.
19. Loo, J. V., J. Cummings, N. Delzenne, H. Englyst, A. Frank, M. Hopkins, N. Kok, G. Macfalane, D. Newton, M. Quigley, M. Roberfroid, T. van Vliet, and E. van den Heuvel. 1999. Functional food properties of non-digestible oligosaccharides: A consensus report from the ENDO project (DGXII AIRII-CT94-1095). Br. J. Nutr. 81: 121-132.
20. Lyness, E. W. and H. W. Doelle. 1983. Levansucrase from Zymomonas mobilis. Biotechnol. Lett. 5: 345-350.
21. Ohtsuka, K., S. Hino, T. Fukushima, O. Ozawa, T. Kanematsu, and T. Uchida. 1992. Characterization of levansucrase from Rahnella aquatilis JCM-1683. Biosci. Biotech. Biochem. 56: 1373-1377.
22. Osborn, M. J., J. E. Gander, E. Parisi, and J. Carson. 1972. Mechanism of assembly of the outer membrane of Salmonella typhimurium isolation and characterization of cytoplasmic and outer membranes. J. Biol. Chem. 247: 3962-3972.
23. Patil, N. K., U. Sharanagouda, J. H. Niazi, C. K. Kim, and T. B. Karegoudar. 2003. Degradation of salicylic acid by free and immobilized cells of Pseudomonas sp. strain NGK1. J. Microbiol. Biotechnol. 13: 29-34.
24. Pelicic, V., J. M. Reyrat, and B. Gicquel. 1996. Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria. J. Bacteriol. 178: 1197-1199.
25. Rhee, S. K., K. B. Song, C. H. Kim, B. S. Park, E. K. Jang, and K. H. Jang. 2002. Levan, pp 351-377. In S. Alexander, E. Vandamme, and S. De. Baets (eds.), Biopolymers, vol. 5. Wiley-VCH, Weinheim, Germany.
26. Salmond, G. P. C. and P. J. Reeves. 1993. Membrane traffic wardens and protein secretion in Gram-negative bacteria. Trends Biochem. Sci. 18: 7-12.
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A.
28. Seo, J. W., K. B. Song, K. H. Jang, C. H. Kim, B. H. Jung, and S. K. Rhee. 2000. Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli. J. Biotechnol. 81: 63-72.
29. Seo, J. W., K. H. Jang, S. A. Kang, K. B. Song, E. K. Jang, B. S. Park, C. H. Kim, and S. K. Rhee. 2002. Molecular cloning of the growth phase-dependent expression of the lsrA gene, encoding levansucrase of Rahnella aquatilis. J. Bacteriol. 184: 5862-5870.
30. Singh, Y. 2003. Photosynthetic activity, and lipid and hydrocarbon production by alginate immobilized cells of Botrycoccus in relation to growth phase. J. Microbiol. Biotechnol. 13: 687-691.
31. Song, K. B., H. Belghith, and S. K. Rhee. 1996. Production of levan, a fructose polymer, using an overexpressed recombinant levansucrase. Ann. N. Y. Acad. Sci. 799: 601-607.
32. Sunita, K., B. H. Chung, K. H. Jang, K. B. Song, C. H. Kim, and S. K. Rhee. 2000. Refolding and purification of Zymomonas mobilis levansucrase produced as inclusion bodies in fed-batch culture of recombinant Escherichia coli. Protein Expres. Purif. 18: 388-393.
33. Tanaka, T., S. Oi, and T. Yamamoto. 1979. Synthesis of levan by levansucrase. J. Biochem. 85: 287-293.
34. Yanase, H., M. Iwata, R. Nakahigashi, K. Kita, N. Kato, and K. Tonomura. 1992. Purification, crystallization and properties of the extracellular levansucrase from Zymomonas mobilis. Biosci. Biotech. Biochem. 56: 1335-1337.