Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli

Journal of Biotechnology

Volumn 81, Issue 1, 2000, Pages 63-72

  Seo, Jeong Woo ^a   Song, Ki Bang ^a   Jang, Ki Hyo ^a   Kim, Chul Ho ^a   Jung, Bong Hyun ^a   Rhee, Sang Ki ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Growth phase dependent expression; Levan; Levansucrase gene (lsrA); Rahnella aquatilis

Indexed keywords

ESCHERICHIA COLI; GROWTH KINETICS; PHASE TRANSITIONS;

MOLECULAR CLONING; THERMOACTIVE LEVANSUCRASE;

CLONING;

LEVANSUCRASE;

ARTICLE; BACTERIAL GROWTH; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE DELETION; GENE ISOLATION; GENE LIBRARY; GENE LOCATION; GENETIC CONSERVATION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RAHNELLA AQUATILIS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE; STRUCTURAL GENE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL DIVISION; CLONING, MOLECULAR; ESCHERICHIA COLI; HEXOSYLTRANSFERASES; MOLECULAR SEQUENCE DATA; RAHNELLA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA; PROKARYOTA; RAHNELLA AQUATILIS;

EID: 0034725942     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(00)00281-9     Document Type: Article

References (30)

1. Arrieta J., Hernandez L., Coego A., Suarez V., Balmori E., Menendez C. et al. Molecular characterization of the levansucrase gene from the endophytic sugarcane bacterium Acetobacter diazotrophicus SRT4. Microbiology. 142:1996;1077-1085.
2. Belghith H., Song K.-B., Kim C.-H., Rhee S.-K. Optimal conditions for levan formation by an overexpressed recombinant levansucrase. Biotechnol. Lett. 18:1996;467-472.
3. Chambert R., Petit-Glatron M.-F. Polymerase and hydrolase activities of Bacillus subtilis levansucrase can be separately modulated by site-directed mutagenesis. Biochem. J. 279:1991;35-41.
4. Chambert R., Treboul G., Dedonder R. Kinetic studies of levansucrase of Bacillus subtilis. Eur. J. Biochem. 41:1974;285-300.
5. Ferrer S., Viejo M.B., Guasch J.F., Enfedaque J., Regue M. Genetic evidence for an activator required for induction of colicin-like bacteriocin 28b production in Serratia marcescens by DNA damaging agents. J. Bacteriol. 178:1996;951-960.
6. Fouet A., Arnaud M., Mer A., Rapoport G. Characterization of the precursor form of the exocellular levansucrase from Bacillus subtilis. Biochem. Biophys. Res. Commun. 119:1984;795-800.
7. Fuqua W.C., Winans S.C., Greenberg E.P. Quorum sensing in bacteria: the LurR-LuxI family of cell density-responsive transcriptional regulators. J. Bacteriol. 176:1994;269-275.
8. Gay P., Le Coq D., Steinmetz M., Ferrari E., Hoch L.A. Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli. J. Bacteriol. 153:1983;1424-1431.
9. Geier G., Geider K. Characterization and influence on virulence of levansucrase gene from the fireblight pathogen Erwinia amylovora. Physiol. Mol. Plant Pathol. 42:1993;387-404.
10. Gross M., Geier G., Rudolph K., Geider K. Levan and levansucrase synthesized by the fireblight pathogen Erwinia amylovora. Physiol. Mol. Plant Pathol. 40:1992;371-381.
11. Hernandez L., Arrieta J., Betancourt L., Falcon V., Madrazo L., Coego A., Menendez C. Levansucrase from Acetobacter diazotrophicus SRT4 is secreted via periplasm by a signal-peptide dependent pathway. Curr. Microbiol. 39:1999;146-152.
12. Hettwer U., Jaeckel F.R., Boch J., Meyer M., Rudolph K., Ullrich M.S. Cloning, nucleotide sequence, and expression in Escherichia coli of levansucrase genes from the plant pathogens Pseudomonas syringae pv. Glycinea and P. syringae pv. phaseolicola. Appl. Environ. Microbiol. 64:1998;3180-3187.
13. Ishihama A. Protein-protein communication within the transcription apparatus. J. Bacteriol. 175:1993;2483-2489.
14. Klier A.F., Rapoport G. Genetics and regulation of carbohydrate catabolism in Bacillus. Ann. Rev. Microbiol. 42:1988;65-95.
15. Kunst E., Rapoport G. Salt stress is an environmental signal affecting degradative enzyme synthesis in Bacillus subtilis. J. Bacteriol. 177:1995;2403-2407.
16. Leigh L.A., Coplin D.L. Exopolysaccharides in plant-bacterial interactions. Annu. Rev. Microbiol. 46:1992;307-346.
17. Li Y., Triccas J.A., Ferenci T. A novel levansucrase-levanase gene cluster in Bacillus stearothermophilus ATCC12980. Biochim. Biophys. Acta. 1353:1997;203-208.
18. O'Mullan P.J., Szakucs-Dobozi M., Eveleigh D.E. Identification of saccharolytic enzymes of Zymomonas mobilis CP4. Biotechnol. Lett. 13:1991;137-142.
19. Ohtsuka K., Hino S., Fukushima T., Ozawa O., Kanematsu T., Uchida T. Characterization of levansucrase from Rahnella aquatilis JCM-1683. Biosci. Biotech. Biochem. 56:1992;1373-1377.
20. Petit-Glatron M.-E., Benyahia E., Chambert R. Secretion of Bacillus subtilis levansucrase: a possible two-step mechanism. Eur. J. Biochem. 163:1987;379-387.
21. Salmond G.P.C., Reeves P.J. Membrane traffic wardens and protein secretion in Gram-negative bacteria. TIBS. 18:1993;7-12.
22. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning. A Laboratory Manual. Second ed:1989;Cold Spring Harbor Press, New York.
23. Sanger R., Nichlen S., Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc. Nad. Acad. Sci. USA. 74:1977;5463-5467.
24. Shimotsu R., Henner D.J. Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes. J. Bacteriol. 168:1986;380-388.
25. Song K.-B., Rhee S.-K. Enzymatic synthesis of levan by Zymomonas mobilis levansucrase overexpressed in Escherichia coli. Biotechnol. Lett. 16:1994;1305-1310.
26. Song K.-B., Joo H.-K., Rhee S.-K. Nucleotide sequence of levansucrase gene (levU) of Zymomonas mobilis ZM1 (ATCC10988). Biochim. Biophys. Acta. 1173:1993;320-324.
27. Song K.-B., Seo J.-W., Kim M.-G., Rhee S.-K. Levansucrase of Rahnella aquatilis ATCC33071: gene cloning, expression, and levan formation. Ann. NY Acad. Sci. 864:1998;506-511.
28. Steinmetz M., Le Coq D., Aymerich S., Gonzy-Treboul G., Gay R. The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control site. Mol. Gen. Genet. 200:1985;220-228.
29. Tang L.B., Lenstra R., Borchert T.V., Nagarajan V. Isolation and characterization of levansucrase-encoding gene from Bacillus amyloliquefaciens. Gene. 96:1990;89-93.
30. Yanase H., Iwata M., Nakahigashi R., Kita K., Kato R., Tonomura K. Purification, crystallization, and properties of the extracellular levansucrase from Zymomonas mobilis. Biosci. Biotech. Biochem. 56:1992;1335-1337.