Sugar radicals in DNA: Isolation of neutral radicals in gamma-irradiated DNA by hole and electron scavenging

Radiation Research

Volumn 163, Issue 5, 2005, Pages 591-602

  Shukla, Lata I ^a,b   Pazdro, Robert ^a   Becker, David ^a   Sevilla, Michael D ^a  

^a OAKLAND UNIVERSITY   (United States)

^b National Centre for Plant Genome Research   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ANION; ARGON; CATION; DEOXYRIBOSE; DNA; GUANINE; IRON; PHOSPHATE; RADICAL; SCAVENGER; SUGAR; CARBOHYDRATE; FERRICYANIDE; FREE RADICAL; POTASSIUM FERRICYANIDE;

ANIMAL CELL; ARTICLE; CHEMICAL BOND; CONTROLLED STUDY; DNA STRAND BREAKAGE; ELECTRON; ELECTRON SPIN RESONANCE; GAMMA IRRADIATION; NONHUMAN; PRIORITY JOURNAL; CARBOHYDRATE METABOLISM; GAMMA RADIATION; METABOLISM; RADIATION EXPOSURE; RADIATION RESPONSE;

ANIMALIA;

CARBOHYDRATE METABOLISM; CARBOHYDRATES; DNA; DOSE-RESPONSE RELATIONSHIP, RADIATION; ELECTRONS; FERRICYANIDES; FREE RADICALS; GAMMA RAYS;

EID: 19444383778     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR3347     Document Type: Article

References (27)

1. D. J. Brenner and C. R. Geard, Links between radiation track structure, radiochemical species and cell survival. In The Early Effects of Radiation on DNA, pp. 33-48. NATO ASI Series, Vol. H54, Springer Verlag, Heidelberg, 1991.
2. J. F. Ward, DNA damage produced by ionizing radiation in mammalian cells: Identities, mechanisms of formation and repairability. Prog. Nucleic Acid Res. Mol. Biol. 35, 95-125 (1988).
3. C. Tronche, B. K. Goodman and M. M. Greenberg, DNA damage induced via independent generation of the radical resulting from for-mal hydrogen abstraction from the C1′-position of a nucleotide. Chem. Biol. 5, 263-271 (1998).
4. W. K. Pogozelski and T. D. Tullius, Oxidative strand scission of nucleic acids: Routes initiated by hydrogen abstraction from the sugar moiety. Chem. Rev. 98, 1089-1107 (1998).
5. W. Wang, M. Yan, D. Becker and M. D. Sevilla, The influence of hydration on the absolute yields of primary free radical in gamma-irradiated DNA at 77 K. II. Individual radical yields. Radiat. Res. 137, 2-10 (1994).
6. B. Weiland and J. Hüttermann, Free radical from X-irradiated dry and hydrated lyophilized DNA as studied by electron spin resonance spectroscopy: Analysis of spectral components between 77K and room temperature. Int. J. Radiat. Biol. 74, 341-358 (1998).
7. J. Hüttermann, M. Roehrig and W. Köhnlein, Free radicals from irradiated lyophilized DNA: Influence of water of hydration. Int. J. Radiat. Biol. 61, 299-313 (1992).
8. S. Gregoli, M. Olast and A. Bertinchamps, Radiolytic pathways in γ-irradiated DNA: Influence of chemical and conformational factors. Radiat. Res. 89, 238-254 (1982).
9. M. D. Sevilla, D. Becker, M. Yan and S. R. Summerfield, Relative abundance of primary radical ions in γ-irradiated DNA: Cytosine vs thymine anions and guanine vs adenine cations. J. Phys. Chem. 95, 3409-3415 (1991).
10. Y. Razskazovskii, M. Roginskaya and M. D. Sevilla, Modification of the reductive pathway in gamma-irradiated DNA by electron scavengers: Targeting the sugar-phosphate backbone. Radiat. Res. 149, 422-432 (1998).
11. L. I. Shukla, R. Pazdro, J. Huang, C. Devreugd, D. Becker and M. D. Sevilla, The formation of DNA sugar radicals from photoexcitation of guanine cation radicals. Radiat. Res. 161, 582-590 (2004).
12. 8+): Evidence for damage to the deoxyribose phosphate backbone. Radiat. Res. 146, 361-368 (1996).
13. B. Weiland and J. Hüttermann, Free radicals from lyophilized 'dry' DNA bombarded with heavy ions as studied by electron spin resonance spectroscopy. Int. J. Radiat. Biol. 75, 1169-1175 (1999).
14. M. G. Debije and W. A. Bernhard, Electron paramagnetic resonance evidence for C3′ sugar radical in crystalline d(CTCTCGAGAG) X-irradiated at 4 K. Radiat. Res. 155, 687-692 (2001).
15. D. Becker, A. Bryant-Friedrich, C. Trzasko and M. D. Sevilla, Electron spin resonance study of DNA irradiated with an argon-ion beam: Evidence for formation of sugar phosphate backbone radicals. Radiat. Res. 160, 174-185 (2003).
16. Y. Razskazovskiy, M. G. Debije and W. A. Bernhard, Strand breaks produced in X-irradiated crystalline DNA: Influence of base sequence. Radiat. Res. 159, 663-669 (2003).
17. S. G. Swarts, D. Becker, M. D. Sevilla and K. T. Wheeler, Radiation-induced damage as a function of hydration. II. Base damage from the electron loss centers. Radiat. Res. 145, 304-314 (1996).
18. .-induced free radicals from purines in acidic glasses. Int. J. Radiat. Biol. 55, 331-345 (1989).
19. Y. Razskazovskiy, M. G. Debije and W. A. Bernhard, Direct radiation damage to crystalline DNA: What is the source of unaltered base release? Radiat. Res. 153, 436-441 (2000).
20. C. von Sonntag, The Chemical Basis of Radiation Biology. Taylor & Francis, Philadelphia, 1987.
21. D. Becker and M. D. Sevilla, The chemical consequences of radiation damage in DNA. Adv. Radiat. Biol. 17, 121-180 (1993).
22. W. Wang, D. Becker and M. D. Sevilla, The influence of hydration on absolute yields of primary free radicals in gamma-irradiated DNA at 77 K. I. Total radical yields. Radiat. Res. 135, 146-154 (1993).
23. D. M. Close, E. O. Hole, E. Sagstuen and W. H. Nelson, EPR and ENDOR studies of x-irradiated single crystals of deoxycytidine 5′-phosphate monohydrate at 10 K and 77 K. J. Phys. Chem. A 102, 6737-6744 (1998).
24. X. Li, M. D. Sevilla and L. Sanche, Density functional theory studies of electron interaction with DNA. Can zero eV electrons induce strand breaks? J. Am. Chem. Soc. 125, 13668-13669 (2003).
25. .OH addition to the DNA bases: Electron affinities and ionization potentials. J. Phys. Chem. 99, 13033-13037 (1995).
26. F. A. Cotton, G. Wilkinson, C. A. Murillo and M. Bochmann, Advanced Organic Chemistry, 6th ed., p. 781. Wiley, New York, 1999.
27. D. M. Close, Where are the sugar radicals in irradiated DNA? Radiat. Res. 147, 663-673 (1997).