The Study of Optimal Conditions of Electroporation in Pseudomonas aeruginosa

Acta Genetica Sinica

Volumn 31, Issue 3, 2004, Pages 311-316

  Shan, Zhi Ying ^a   Xu, Hai Jin ^a   Shi, Xing Qi ^a   Nie, Zhou ^a   Yu, Yan ^a   Zhang, Xiu Ming ^a   Bai, Yan Ling ^a   Qiao, Ming Qiang ^a   Gao, Cai Chang ^a  

^a NANKAI UNIVERSITY   (China)

Author keywords

Electroporation; Mu trsnsposon complexes; Pseudomonas aeruginosa

Indexed keywords

DNA;

ARTICLE; BACTERIAL STRAIN; CONCENTRATION RESPONSE; ELECTRIC POTENTIAL; ELECTROPORATION; NONHUMAN; PLASMID; PSEUDOMONAS AERUGINOSA; TRANSPOSON;

DNA TRANSPOSABLE ELEMENTS; ELECTROPORATION; HUMANS; PSEUDOMONAS AERUGINOSA; TRANSFORMATION, BACTERIAL; TRANSPOSASES;

BACTERIA (MICROORGANISMS); INSERTION SEQUENCES; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; TRANSPOSONS;

EID: 1942537096     PISSN: 03794172     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Thien-Fah C M, O'Toole G A. Mechanisms of biofilm resistance to antimicrobial agents. Trends in Microbiology, 2001, 9 (1): 34-38.
2. Lamberg A, Nieminen S, Qiao M Q, Savilahti H. Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage Mu. Appl Environ Microbiol, 2002, 68: 705-712.
3. Mattick J S, Bills M M, Anderson B J. Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa. J Bacteriol, 1987, 169: 33-41.
4. Anthony W S, Barbara H I. Transformation of Pseudomonas aeruginosa by electroporation. Nucleic Acids Research, 1989, 17 (24): 10509.
5. Diver J M, Bryan L E, Sokol P A. Transformation of Pseudomonas aeruginosa by electroporation. Anal Biochem, 1990, 189(1): 75-79.
6. Farinha M A, Kropinski A M. High efficiency electroporation of Pseudomonas aeruginosa using frozen cell suspensions. FEMS Microbiol Lett, 1990, 58(2): 221-225.
7. Ausubel F M, Brent R, Kingston R E, Moore D D, Seidman J G, Smith J A, Struhl K. Short Protocols in Molecular Biology. 3rd ed. John Wiley & Sons, Inc, Beijing: 1995.
8. Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold spring Harbor Laboratory Press. Beijing: 1989.
9. Croft L, Beatson S A, Whitchurch C B, Huang B, Blakelaey R L, Mattick J S. An interactive web-based Pseudomonas aeruginosa genome database: discovery of new genes, pathways and structures. Microbiology, 2000, 46: 2351-2364.
10. Stover C K, Pham X Q, Erwin A L, Mizoguchi S D, Warrener P, Hickey M J, Brinkmann F S, Hufnagle W O, Kowalik D J, Lagrou M, Garber R L, Goltry L, Tolentino E, Westbrock M S, Yuan Y, Brody L L, Coulter S N, Folger K R, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong G K, Wu Z, Paulsen I T, Reizer J, Saier M H, Hancock R E, Lory S, Olson M V. Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature, 2000, 406: 959-964.
11. Savilahti H, Rice P A, Mizuuchi K. The phage Mu transpososome core: DNA requirments for assembly and function. The EMBO journal, 1995, 14: 4893-4903.
12. Whiteley M, Bangera M G, Bumgarner R E, Parsek M R, Teizel G M, Lory S, Greenberg E P. Gene expression in Pseudomonas aeruginosa biofilms. Nature, 2001, 143: 860-864.