Cloning and functional characterization of the SUR2/SYR2 gene encoding sphinganine hydroxylase in Pichia ciferrii

Yeast

Volumn 21, Issue 5, 2004, Pages 437-443

  Bae, Jung Hoon ^a,c   Sohn, Jung Hoon ^a   Park, Chang Seo ^b   Rhee, Joon Shick ^c   Choi, Eui Sung ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Doosan Corporation   (South Korea)

^c Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Hydroxylation; Pichia ciferrii; SUr2; Syringomycin E

Indexed keywords

OXYGENASE; PHYTOSPHINGOSINE; SPHINGANINE; SYRINGOMYCIN E;

ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; FUNGAL STRAIN; FUNGUS MUTANT; GENBANK; GENE EXPRESSION; GENE FUNCTION; GENE ISOLATION; GENETIC CODE; HYDROXYLATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; NULL ALLELE; PHENOTYPE; PICHIA; PICHIA CIFERRII; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, FUNGAL; DRUG RESISTANCE, FUNGAL; GENE DELETION; GENES, FUNGAL; GENETIC COMPLEMENTATION TEST; GENETIC VECTORS; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; PICHIA; RESTRICTION MAPPING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPHINGOSINE;

PICHIA; PICHIA CIFERRII; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; SPHINGIODEA;

EID: 1942508133     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.1082     Document Type: Article

References (22)

1. Adetuyi FC, Isogai A, Di Giorgio D, Ballio A, Takemoto JY. 1995. Saprophytic Pseudomonas syringae strain M1 of wheat produces cyclic lipodepsipeptides. FEMS Microbiol Lett 131: 63-67.
2. Alani E, Cao L, Kleckner N. 1987. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545.
3. Bae JH, Sohn JH, Park CS, Rhee JS, Choi ES. 2003. Integrative transformation system for the metabolic engineering of the sphingoid base producing yeast Pichia ciferrii. Appl Environ Microbiol 69: 812-819.
4. Barenholz Y, Gadot N, Valk E, Gatt S. 1973. Identification of the enzymatic lesions responsible for the accumulation of acetylated sphingosine bases in the yeast Hansenula ciferrii. Biochim Biophys Acta 306: 341-345.
5. Cliften P, Wang Y, Mochizuki D. et al. 1996. SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae. Microbiology 142: 477-484.
6. Gietz RD, Schiestl RH, Willems AR, Woods RA. 1995. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11: 355-360.
7. Grilley MM, Stock SD, Dickson RC, Lester RL, Takemoto JY. 1998. Syringomycin action gene SYR2 is essential for sphingolipid 4-hydroxylation in Saccharomyces cerevisiae. J Biol Chem 273: 11 062-11 068.
8. Haak D, Gable K, Beeler T, Dunn T. 1997. Hydroxylation of Saccharomyces cerevisiae ceramides requires Sur2p and Scs7p. J Biol Chem 272: 29 704-29 710.
9. Hannun YA. 1996. Functions of ceramide in coordinating cellular responses to stress. Science 274: 1855-1859.
10. Holm C, Meeks-Wagner DW, Fangman WL, Botstein D. 1986. A rapid, efficient method for isolating DNA from yeast. Gene 42: 169-173.
11. Inoue H, Nojima H, Okayama H. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96: 23-28.
12. Karlsson KA. 1970. On the chemistry and occurrence of sphingolipid long-chain bases. Chem Phys Lipids 5: 6-43.
13. Kok JW, Nikolova-Karakashian M, Klappe K, Alexander C, Merrill AH Jr. 1997. Dihydroceramide biology. Structure-specific metabolism and intracellular localization. J Biol Chem 272: 21 128-21 136.
14. Lester RL, Dickson RC. 1993. Sphingolipids with inositolphosphate-containing head groups. Adv Lipid Res 26: 253-274.
15. Letourneur F, Gaynor EC, Hennecke S, et al. 1994. Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum. Cell 79: 1199-1207.
16. Lu P, Davis BP, Hendrick J, Jeffries TW. 1998. Cloning and disruption of the β-isopropylmalate dehydrogenase gene (LEU2) of Pichia stipitis with URA3 and recovery of the double auxotroph. Appl Microbiol Biotechnol 49: 141-146.
17. Mathias S, Pena LA, Kolesnick RN. 1998. Signal transduction of stress via ceramide. Biochem J 335: 465-480.
18. Michel C, van Echten-Deckert G, Rother J, Sandhoff K, Wang E, Merrill AH Jr. 1997. Characterization of ceramide synthesis. A dihydroceramide desaturase introduces the 4,5-trans-double bond of sphingosine at the level of dihydroceramide. J Biol Chem 272: 22 432-22 437.
19. Min JK, Yoo HS, Lee EY, Lee WJ, Lee YM. 2002. Simultaneous quantitative analysis of sphingoid base 1-phosphates in biological samples by o-phthalaldehyde precolumn derivatization after dephosphorylation with alkaline phosphatase. Anal Biochem 303: 167-175.
20. Rothstein RJ. 1983. One-step gene disruption in yeast. Methods Enzymol 101: 202-211.
21. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press: New York.
22. Spiegel S, Merrill AH Jr. 1996. Sphingolipid metabolism and cell growth regulation. FASEB J 10: 1388-1397.