Cloning and disruption of the β-isopropylmalate dehydrogenase gene (LEU2) of Pichia stipitis with URA3 and recovery of the double auxotroph

Applied Microbiology and Biotechnology

Volumn 49, Issue 2, 1998, Pages 141-146

  Lu, P ^a,c   Davis, B P ^b,d   Hendrick, J ^b,e   Jeffries, T W ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b FOREST PRODUCTS LABORATORY   (United States)

^c Scriptgen Pharmaceuticals Inc   (United States)

^d UNIVERSITY OF COLORADO   (United States)

^e Ophidian Pharmaceuticals   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; AUXOTROPHIC MUTANT; CARBOHYDRATE METABOLISM; CELL TRANSFORMATION; ENDOMYCETALES; FERMENTATION; GENE CONSTRUCT; GENE LIBRARY; GENE TRANSFER; MOLECULAR CLONING; SEQUENCE ANALYSIS;

3-ISOPROPYLMALATE DEHYDROGENASE; ALCOHOL OXIDOREDUCTASES; BLOTTING, SOUTHERN; CLONING, MOLECULAR; DNA, FUNGAL; DRUG RESISTANCE, MICROBIAL; FUNGAL PROTEINS; GENES, FUNGAL; GENETIC VECTORS; MUTAGENESIS, INSERTIONAL; OROTIC ACID; PICHIA; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, GENETIC;

PICHIA STIPITIS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 0031885474     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051150     Document Type: Article

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